Clinical and Experimental Reasoning of the Use of Collapan-L and Thymogen, and Their Combinations in the Treatment of Fractures of Tubular Bones in White Rats

Abstract

This paper presents the results of the use of collapan-L and thymogen, as well as their combinations, on the processes of reparative osteogenesis in experimental femur fractures in rats. It was found that their use significantly reduced the negative impact of the fracture on the main markers of bone metabolism - calcium, phosphorus, alkaline phosphatase. The most significant changes were recorded in the fourth group, where we used the combined treatment of drugs, an immunostimulator immediately after surgery for a course of 5 days. The results of X-ray and histological studies also indicate more active processes of osteogenesis and reparative regeneration of bone tissue in the fourth experimental group. Thus, on the 45th day after surgery, the callus was homogeneous, the fracture line was barely visible. In addition, a higher proportion of bone and a low content of connective tissue at the fracture site were noted compared to other groups of animals. Thus, in comparison to the control group of animals, the content of bone tissue in the fourth experimental group is higher by 8.86%, and the connective tissue is lower by 46.06%. The results obtained in determining the strength of bone to mechanical stress also indicate that the most effective method for stimulating reparative osteogenesis in fractures is the combined use of collapan-L and thymogen, starting immediately after surgery for five days. Thus, on the 45th day after surgery, the strength of the femur in the fourth group reached 53.02% of the intact level, which was 9.39% higher than the control values.