Clinical and epidemiological features of Chryseobacterium indologenes infections: Analysis of 215 cases

Chen, Fu Lun; Wang, Giueng Chueng; Teng, Sing On; Ou, Tsong Yih; Yu, Fenglei; Lee, Wen Sen

doi:10.1016/j.jmii.2012.08.007

Cited by 119 publications

(154 citation statements)

References 23 publications

Supporting

Mentioning

127

Contrasting

Unclassified

Order By: Relevance

“…C. oranimense has been reported to be isolated from raw milk (30), yet this is the first report of C. oranimense in humans, i.e., from a CF patient. Chryseobacterium species are multidrug resistant, with most intrinsically resistant to penicillin, first-and second-generation cephalosporin, aztreonam (14,33), and colistin (16), and have been reported to be acquired nosocomially via medical devices and contaminated water supplies in hospitals (18). Using a polyphasic approach, some studies have reported the presence of unusual bacteria, such as Acinetobacter spp., Bordetella spp., Comamonas spp., Rhizobium spp., Herbaspirillum spp., Moraxella spp., I. limosus, and Chryseobacterium spp., in the sputum samples from CF patients (34).…”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

“…There are about 283 reported cases of infections associated with C. indologenes (15,16). In a report by Chen et al, 215 clinical isolates of multidrug-resistant C. indologenes were identified after the increasing clinical use of colistin and tigecycline (16), a risk for patients who have undergone extensive administration of antibiotics for a long period (17). Although the source of infection of this microbe is not clear, it has been reported to be acquired nosocomially via medical devices and contaminated water supplies in hospitals (18).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Whole-Genome Sequence of Chryseobacterium oranimense, a Colistin-Resistant Bacterium Isolated from a Cystic Fibrosis Patient in France

Sharma

Gupta

Diène

et al. 2015

Antimicrob Agents Chemother

View full text Add to dashboard Cite

For the first time, we report the whole-genome sequence analysis of Chryseobacterium oranimense G311, a multidrug-resistant bacterium, from a cystic fibrosis patient in France, including resistance to colistin. Whole-genome sequencing of C. oranimense G311 was performed using Ion Torrent PGM, and RAST, the EMBL-EBI server, and the Antibiotic Resistance Gene-ANNOTation (ARG-ANNOT) database were used for annotation of all genes, including antibiotic resistance (AR) genes. General features of the C. oranimense G311 draft genome were compared to the other available genomes of Chryseobacterium gleum and Chryseobacterium sp. strain CF314. C. oranimense G311 was found to be resistant to all ␤-lactams, including imipenem, and to colistin. The genome size of C. oranimense G311 is 4,457,049 bp in length, with 37.70% GC content. We found 27 AR genes in the genome, including ␤-lactamase genes which showed little similarity to the known ␤-lactamase genes and could likely be novel. We found the type I polyketide synthase operon followed by a zeaxanthin glycosyltransferase gene in the genome, which could impart the yellow pigmentation of the isolate. We located the O-antigen biosynthesis cluster, and we also discovered a novel capsular polysaccharide biosynthesis cluster. We also found known mutations in the orthologs of the pmrA (E8D), pmrB (L208F and P360Q), and lpxA (G68D) genes. We speculate that the presence of the capsular cluster and mutations in these genes could explain the resistance of this bacterium to colistin. We demonstrate that whole-genome sequencing was successfully applied to decipher the resistome of a multidrug resistance bacterium associated with cystic fibrosis patients. Cystic fibrosis (CF) is the most common autosomal recessive genetic disease and results from mutations within the gene coding for the cystic fibrosis transmembrane regulator (CFTR) protein. This life-threatening disease affects all racial and ethnic groups, though it is more common among Caucasians (1, 2). CF is characterized by hyperproduction of viscous mucus by the affected glands, resulting mainly in impaired respiratory and pancreatic functions. The most common complication of CF involves the chronic respiratory infections caused by bacterial pathogens (3), which are the main reason for the high morbidity and mortality of the disease (4). Traditionally, only a few bacteria were involved in CF lung infections, including Staphylococcus aureus, Pseudomonas aeruginosa, Haemophilus influenzae, and Streptococcus pneumoniae. However, many new or emerging opportunistic bacteria have been described in CF patients over the past decade, for instance, Burkholderia cepacia complex, Stenotrophomonas maltophilia, Achromobacter xylosoxidans, Pandoraea spp., Ralstonia spp., Inquilinus limosus, and nontuberculosis mycobacteria, as well as fungi (5). Chronic microbial infection, along with P. aeruginosa infections, leads to excessive airway inflammation and the eventual loss of pulmonary function. Colistin is an extremely important antibiotic used in ...

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Whole-Genome Sequence of Chryseobacterium oranimense, a Colistin-Resistant Bacterium Isolated from a Cystic Fibrosis Patient in France

Sharma

Gupta

Diène

et al. 2015

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…The isolated colonies produced a yellow pigment although this property is characteristic for different species of Chryseobacterium (Bernardet et al 2005). It is known that Chryseobacterium is most commonly found in environments including water sources (Chen et al 2012). More recently, a number of Chryseobacterium spp.…”

Section: Discussionmentioning

confidence: 99%

Gut microbiota isolated from the European pond turtle (Emys orbicularis) and its antimicrobial resistance

Ružauskas¹,

Misyte²,

Vaškevičiūtė³

et al. 2016

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

The aim of the study was to isolate cultivable gut microbiota from European pond turtles kept at the Lithuanian Zoo and to determine antimicrobial resistance of the isolates. The study subjects included 8 elderly turtles living at the Lithuanian Zoo for about 50 years as well as their offspring -24 young individuals (1-2 years old) that were hatched at the same zoo. Animals were not exposed by treatment with antimicrobials during the last 3 years.Gut samples were taken from the cloaca and inoculated onto universal media. Isolates then were identified using sequence analysis of 16S rRNA.The antimicrobial susceptibility testing was performed using the agar diffusion method according to Kirby-Bauer. Clinical breakpoints according to CLSI whenever possible, were used for interpretation of susceptibility. Bacterial isolates resistant to at least three antimicrobials of different classes were treated as multi-resistant.Fifty-two bacterial isolates were obtained and identified from turtle gut samples. The most prevalent genera included Aeromonas, Chryseobacterium and Citrobacter. Fifty percent of the isolates obtained from elderly turtles (CI 95% -19.01-80.99) and 54.8% (CI 95% -39.75-69.85) of the isolates from young animals were identified as multi-resistant. The most common resistance rates of the isolates from both groups of the turtles were observed toward ampicillin (86.6%), ciprofloxacin (61.5%) and gentamicin (40.4%). The lowest number of resistant isolates were detected toward combination of sulfamethoxazole-trimethoprim (26.9%). The study revealed that European pond turtles kept in captivity are carriers of multi-resistant bacteria however, further studies need to be performed to investigate whether the resistant microorganisms are natural microbiota for this species or they were acquired in the zoo.

show abstract

“…által fertőzött CF-betegek nagyobb valószínűséggel fertőzöttek P. aeruginosával vagy a B. cepacia complex valamely tagjával is [13]. Karbapenemekkel és aminoglikozidokkal szemben természetes rezisztenciát mutatnak [13,14]. A CIP, LEV, SXT és FEP eseteink ≥90%-ában hatékonynak bizonyult.…”

Section: Fontosabb Ritkábban Előforduló Gram-negatív Pálcák Jellemzéseunclassified

Ritkábban előforduló, alsó légúti fertőzést okozó Gram-negatív nem fermentáló pálcák

et al. 2018

View full text Add to dashboard Cite

Bevezetés: A nem fermentáló Gram-negatív baktériumok a környezetben széles körben elterjedtek. Többségükkel opportunista, nosocomialis patogénként találkozunk a klinikai gyakorlatban. A csoport jól ismert tagjai (például a Pseudomonas vagy az Acinetobacter fajok) mellett ritkább fajok is identifikálásra kerülnek, különösen, mióta a klinikai mikrobiológiai gyakorlatba bevezetésre került a "mátrix-asszisztált lézer deszorpciós-ionizációs, repülési idő mérésén alapuló tömegspektrometria" (MALDI-TOF MS) technika. A ritkábban előforduló Gram-negatív baktériumok dön-tő része légúti mintákból kerül izolálásra. Klinikai jelentőségük az alsó légúti fertőzésekben, akárcsak klinikai mikrobiológiai vizsgálatuk szabályai, egyelőre tisztázatlanok. Célkitűzés: Néhány fontosabb, ritkábban előforduló, alsó légúti fertőzést okozó Gram-negatív pálca klinikai mikrobiológiai jellemzése. Módszer: Négy év adatainak retrospektív feldolgozásával áttekintettük a nem fermentáló Gram-negatív baktériumok alsó légúti fertőzésekben potenciálisan betöltött szerepét és antibiotikum-érzékenységét a Semmelweis Egyetemen. Összesen 3589 beteg alsó légúti mintáit elemeztük a 2013-2016 négyéves periódusban. A baktériumok azonosítása minden esetben MALDI-TOF MS módszerrel, az antibiotikum-érzékenységi vizsgálat pedig döntően korongdiffú-ziós módszerrel történt. Eredmények: A Pseudomonas aeruginosát követően a Stenotrophomonas maltophilia volt a második, míg az Acinetobacter baumannii a harmadik leggyakrabban azonosított nem fermentáló Gram-negatív baktérium az alsó légúti minták-ban. Összesen 742 olyan izolátumot azonosítottunk, melyek a ritka nem fermentáló pálcák csoportjába tartoztak. Az izolátumok 23%-a Achromobacter xylosoxidans volt. A Chryseobacterium, Rhizobium, Delftia és Elizabethkingia fajok mellett néhány Ralstonia és Ochrobactrum, valamint egy-egy egyéb baktériumfajt azonosítottunk. A pontos fajazonosítás kiemelt jelentőségű, mivel e baktériumok nagy része természetes aminoglikozid-rezisztenciával bír. Gyakran rezisztensek ceftazidimre, cefepimre, piperacillin/tazobactamra és karbapenemekre is. Következtetések: Összességében a ciprofloxacin, a levofloxacin és a trimethoprim/sulfamethoxazol bizonyult a leghatékonyabbnak a csoport tagjaival szemben. Orv Hetil. 2018; 159(1): 23-29.

show abstract

Clinical and epidemiological features of Chryseobacterium indologenes infections: Analysis of 215 cases

Cited by 119 publications

References 23 publications

Whole-Genome Sequence of Chryseobacterium oranimense, a Colistin-Resistant Bacterium Isolated from a Cystic Fibrosis Patient in France

Whole-Genome Sequence of Chryseobacterium oranimense, a Colistin-Resistant Bacterium Isolated from a Cystic Fibrosis Patient in France

Gut microbiota isolated from the European pond turtle (Emys orbicularis) and its antimicrobial resistance

Ritkábban előforduló, alsó légúti fertőzést okozó Gram-negatív nem fermentáló pálcák

Contact Info

Product

Resources

About