Clinical and epidemiological analysis of Campylobacter fetus subsp. fetus infections in humans and comparative genetic analysis with strains isolated from cattle

Escher, Robert; Brunner, Colette; Steiger, Niklaus von; Brodard, Isabelle; Droz, Sara; Abril, Carlos; Kuhnert, Peter

doi:10.1186/s12879-016-1538-7

Cited by 16 publications

(14 citation statements)

References 46 publications

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“…No antimicrobial resistance to streptomycin, penicillin, tetracycline and enrofloxacin was found in the 13 Cfv isolates, and streptomycin and tetracycline resistance genes were not detected in the 31 Cfv analysed genomes. These latter genes were identified in Cff strains harbouring the Cfv-associated genomic island with T4SS encoding genes [11,19]. The antimicrobial susceptibility results of this study are in accordance with a previous study, in which all isolates were susceptible to penicillin, streptomycin and tetracycline and only 5% of the isolates were susceptible to enrofloxacin [43].…”

Section: Discussionsupporting

confidence: 91%

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Genomic and Phenotypic Characterization of Campylobacter fetus subsp. venerealis Strains

et al. 2021

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The pathogenesis mechanisms of Campylobacter fetus subsp. venerealis (Cfv), the etiologic agent of Bovine Genital Campylobacteriosis remain elusive. This study evaluated the virulence potential and biovar characteristics of Cfv isolates (n = 13) by PCR screening of putative virulence-factor (VF) genes, Multilocus Sequence Typing (MLST) analysis, antimicrobial susceptibility to tetracycline, penicillin, enrofloxacin and streptomycin testing and whole-genome sequencing (WGS; n = 5), also comparing the latter with 26 other whole-genome sequences of Cfv strains. The putative VF genes encoding type IV secretion system of Cfv (virB2-virB11/virD4) were absent in 92% of isolates, including isolates from aborted foetuses, evidencing that these VF genes are not essential for Cfv pathogenicity. The parA gene, used as a Cfv diagnostic molecular target, was detected in only 3 of 13 isolates, invalidating its use for diagnosis purposes. Three novel sequence types were identified by MLST. Although no in vitro antimicrobial resistance was detected, WGS identified antimicrobial resistance-related genes, including those encoding the multidrug efflux pumps CmeABC and YkkCD, indicating that their presence is not enough to provide antimicrobial resistance. The SNP and accessory protein families analysis segregated the Cfv and Cfv biovar intermedius (Cfvi) strains into different clusters. In conclusion, this study evidenced virulence potential and biovar characteristics of Cfv and Cfvi, which are of relevance for the control of Bovine Genital Campylobacteriosis.

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Section: Discussionsupporting

confidence: 91%

“…However, the prevalence of antimicrobial resistance among Cfv isolates has been poorly investigated. Indeed, a genomic island with two genes involved in tetracycline and streptomycin resistance was identified in Cff isolates [11,19], but its occurrence in Cfv is unknown.…”

Section: Introductionmentioning

confidence: 99%

Genomic and Phenotypic Characterization of Campylobacter fetus subsp. venerealis Strains

et al. 2021

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“…The clonal nature of C. fetus might be attributed to higher genetic stability of this pathogen as compared to other Campylobacter species [20,21]. Thus, there is a significant possibility for the continuous circulation of few genotypes of C. fetus in this endemic region of Argentina.…”

Section: Discussionmentioning

confidence: 99%

Phylogenomic analysis for Campylobacter fetus ocurring in Argentina

et al. 2021

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Background and Aim: Campylobacter fetus is one of the most important pathogens that severely affects livestock industry worldwide. C. fetus mediated bovine genital campylobacteriosis infection in cattle has been associated with significant economic losses in livestock production in the Pampas region, the most productive area of Argentina. The present study aimed to establish the genomic relationships between C. fetus strains, isolated from the Pampas region, at local and global levels. The study also explored the utility of multi-locus sequence typing (MLST) as a typing technique for C. fetus. Materials and Methods: For pangenome and phylogenetic analysis, whole genome sequences for 34 C. fetus strains, isolated from cattle in Argentina were downloaded from GenBank. A local maximum likelihood (ML) tree was constructed and linked to a Microreact project. In silico analysis based on MLST was used to obtain information regarding sequence type (ST) for each strain. For global phylogenetic analysis, a core genome ML-tree was constructed using genomic dataset for 265 C. fetus strains, isolated from various sources obtained from 20 countries. Results: The local core genome phylogenetic tree analysis described the presence of two major clusters (A and B) and one minor cluster (C). The occurrence of 82% of the strains in these three clusters suggested a clonal population structure for C. fetus. The MLST analysis for the local strains revealed that 31 strains were ST4 type and one strain was ST5 type. In addition, a new variant was identified that was assigned a novel ST, ST70. In the present case, ST4 was homogenously distributed across all the regions and clusters. The global analysis showed that most of the local strains clustered in the phylogenetic groups that comprised exclusively of the strains isolated from Argentina. Interestingly, three strains showed a close genetic relationship with bovine strains obtained from Uruguay and Brazil. The ST5 strain grouped in a distant cluster, with strains obtained from different sources from various geographic locations worldwide. Two local strains clustered in a phylogenetic group comprising intercontinental Campylobacter fetus venerealis strains. Conclusion: The results of the study suggested active movement of animals, probably due to economic trade between different regions of the country as well as with neighboring countries. MLST results were partially concordant with phylogenetic analysis. Thus, this method did not qualify as a reliable subtyping method to assess C. fetus diversity in Argentina. The present study provided a basic platform to conduct future research on C. fetus, both at local and international levels.

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“…Further characterization of the isolates based on gene encoding for the bacterial outer membrane protein content is known to improve Campylobacter epidemiological identification through genetic discrimination [ 97 ]. Specifically, for C. jejuni and C. coli , the presence of the the porA gene encoding for the MOMP porin A has been demonstrated, while for C. fetus the genes cmp1 and cmp2 encode for two porin-like activity MOMPs other than porin A [ 97 , 98 ] . Regional outbreak investigations regarding foodborne human campylobacteriosis underline the usefulness of MOMP typing to triage environmental Campylobacter isolates before conducting more laborious molecular typing analysis [ 99 ].…”

Section: Molecular Typing Tools: Getting To Know Each Othermentioning

confidence: 99%

Inquiring into the Gaps of Campylobacter Surveillance Methods

Magana

Chatzipanagiotou

Burriel

et al. 2017

Veterinary Sciences

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Campylobacter is one of the most common pathogen-related causes of diarrheal illnesses globally and has been recognized as a significant factor of human disease for more than three decades. Molecular typing techniques and their combinations have allowed for species identification among members of the Campylobacter genus with good resolution, but the same tools usually fail to proceed to subtyping of closely related species due to high sequence similarity. This problem is exacerbated by the demanding conditions for isolation and detection from the human, animal or water samples as well as due to the difficulties during laboratory maintenance and long-term storage of the isolates. In an effort to define the ideal typing tool, we underline the strengths and limitations of the typing methodologies currently used to map the broad epidemiologic profile of campylobacteriosis in public health and outbreak investigations. The application of both the old and the new molecular typing tools is discussed and an indirect comparison is presented among the preferred techniques used in current research methodology.

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Clinical and epidemiological analysis of Campylobacter fetus subsp. fetus infections in humans and comparative genetic analysis with strains isolated from cattle

Cited by 16 publications

References 46 publications

Genomic and Phenotypic Characterization of Campylobacter fetus subsp. venerealis Strains

Genomic and Phenotypic Characterization of Campylobacter fetus subsp. venerealis Strains

Phylogenomic analysis for Campylobacter fetus ocurring in Argentina

Inquiring into the Gaps of Campylobacter Surveillance Methods

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