Clinical and endocrinological investigations on superovulation induced in heifers by human menopausal gonadotrophin

Abstract

After an i.v. injection of human menopausal gonadotrophin (hMG), three components of the disappearance time in heifers were found for immunoreactive LH (half-life 13.8--1020 min) and immunoreactive FSH (half-life 21.3--1090 min). When heifers were treated daily with hMG from Days 9, 10 or 11 of the cycle for 3 or 5 days, a total dose of 1350 i.u. FSH induced 10.2 +/- 2.5 (mean +/- s.d.) corpora lutea (CL) in 6 animals and 13 persistent follicles in 4 animals, while a total dose of 2100 i.u. FSH induced 14.3 +/- 1.5 CL in 6 heifers and there were no persistent follicles. Nine heifers treated with a single i.m. injection of 1500 i.u. PMSG exhibited 11.4 +/- 8.6 CL with 17 persistent follicles in 7 animals. Progesterone concentrations rose significantly faster and the oestradiol drop was more rapid after oestrus in heifers treated with hMG than in those treated with PMSG. These results demonstrate that stimulation of the ovarian follicles of heifers is more homogeneous when hMG is used.

Ovarian Follicular Dynamics. A review with Emphasis on the Bovine Species. Part II: Antral Development, Exogenous Influence and Future Prospects

“…In human gynecology, hMG is often employed for infertility treatment, and it is characterized by a strong FSH and weak LH activity (Mori 1994). The first report on the use of hMG in bovine superovulation dates from 1982 (Lauria et al. 1982).…”

Reproductive performance of female goats given progestagen associated with PMSG and/or HMG in deep anestrus

“…Except for group A goats, the percentage of non-pregnant females with high plasma progesterone levels 20 to 22 days after mating gave an overall accuracy similar to that reported by Thimonier et al (1977), Chiesa et al (1981 and Corteel et al (1984). We cannot explain the high percentage of non-pregnant animals in group A that were diagnosed as pregnant and can only hypothesize that, because of its long half-life (Lauria et al, 1982), PMSG induced a prolonged stimulation of the ovaries (Tamanini et al, 1985), which possibly resulted in excessive luteinization. Plasma Irrespective of the treatment, none of the non-pregnant goats in our study showed behavioral estrus or conceived when males were introduced 20 to 22 days after induced estrus ; this suggests non-return to estrus.…”

“…On the contrary, the administration of human menopausal gonadotropin (HMG) ensured good estrus synchronization, as reflected by plasma luteinizing hormone (LH) profiles (Tamanini et al, 1985) ; this was due to the short half-life of HMG as compared with PMSG (Schams et al, 1978 ;Lauria et al, 1982 (Masaki and Masuda, 1968).…”

Optimum Dose of LH-RH Analogue Fertirelin Acetate for the Induction of Superovulation in Mice

“…Gonadotrophins such as follicle-stimulating hormone (FSH) [7,9,11], pregnant mare serum gonadotrophin (PMSG) [2], and human menopausal gonadotrophin (HMG) [6,8] have been used for the development of ovarian follicles in superovulation induction. We first reported that an analogue of luteinizing hormone-releasing hormone (LH-RH) can be used for inducing follicular development [4], and have been investigating the time of effective adminis-tration of the analogue using mice [5].…”