Clinical and diagnostic value of ribosomal P autoantibodies in systemic lupus erythematosus

Haddouk, Samy; Marzouk, S.; Jallouli, M.; Fourati, H.; Frigui, M.; Hmida, Y. Ben Hadj; Koubaa, Faten; Sellami, W.; Baklouti, S.; Hachicha, J.; Bahloul, Z.; Masmoudi, Hatem

doi:10.1093/rheumatology/kep142

Cited by 44 publications

(34 citation statements)

References 38 publications

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“…We found a weak association between anti-Rib-P aabs and arthritis, which is consistent with the outcome of a recent study [33] demonstrating a significant association between anti-Rib-P antibodies and arthritis but no correlation with other specific clinical manifestations of SLE. This original association points out the necessity of further clinical studies and encourages the design of studies that address the issue of whether anti-Rib-P aabs participate in the development of arthritis.…”

Section: Association With Age and Clinical Parameterssupporting

confidence: 92%

Multi-center evaluation of autoantibodies to the major ribosomal P C22 epitope

et al. 2010

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Anti-ribosomal P (Rib-P) autoantibodies have been demonstrated to be a specific diagnostic marker for systemic lupus erythematosus (SLE). The aim of this study was to evaluate the prevalence of anti-Rib-P (C22) antibodies in patients with SLE drawn from international, multi-center clinics. Sera collected from patients with SLE (n = 333) and various controls (n = 397) in four centers were tested for anti-C22 autoantibodies by ELISA (Dr. Fooke Laboratorien). SLE activity index 2000 (SLEDAI-2K) was assessed for each patient in two centers. Autoantibody profiles were generated for the SLE samples from Canada using two profile assays. Using the manufacturer`s cut-off value, the prevalence of anti-C22 autoantibodies in patients with SLE between the participating centers varied from 18.2 to 29.0%. In the control sera, the prevalence of anti-C22 autoantibodies was low and the titer in the individual control groups varied significantly. In patients with connective tissue disease other than SLE and in patients with infections disease, the anti-C22 reactivity was significantly higher than in healthy controls (P < 0.0001). Overall sensitivity/specificity was 23.1/99.0%, respectively. Anti-Rib-P reactivity was significantly higher in young (mean age 33.9 vs. 45.3 years) SLE patients (P < 0.0001) and was associated with decreased C3 (P = 0.0335) and C4 levels (P = 0.0129). Moderate association between anti-C22 reactivity and SLEDAI-2K was observed in one cohort (P = 0.02). Anti-C22 autoantibodies are frequently and specifically found in patients with SLE. Although an association between anti-C22 reactivity and SLEDAI score was observed in one center, measurement of anti-C22 autoantibodies is likely not appropriate for measuring global disease activity.

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Section: Association With Age and Clinical Parameterssupporting

confidence: 92%

Multi-center evaluation of autoantibodies to the major ribosomal P C22 epitope

et al. 2010

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“…We have also recently studied a patient with lupus nephritis of membraneous type [International Society of Nephrology/Renal Pathology Society (ISN/RPS) 2004 classification class V], in whom anti-P, but neither anti-Sm nor antidsDNA, was positive (data not shown). This observation underscores the role of anti-P in the pathogenesis of lupus nephritis as well as the diagnostic value of anti-P in SLE, especially in patients without anti-Sm and anti-dsDNA [41].…”

Section: Role Of Anti-p In Lupus Nephritissupporting

confidence: 58%

“…Of note, anti-P have been detected in SLE patients without anti-DNA, anti-Sm [41], or anti-nuclear antibodies [42]. In the latter patient, the intensity of proteinuria was correlated with anti-P titers, suggesting the role of anti-P in nephritis.…”

Section: Role Of Anti-p In Lupus Nephritismentioning

confidence: 88%

Anti-ribosomal P antibodies and lupus nephritis

Hirohata

2011

Clin Exp Nephrol

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Increasing attention has been paid to the relationship of autoantibodies to ribosomal P proteins (anti-P) with lupus nephritis. Several mechanisms of involvement of anti-P in lupus nephritis have been proposed, including cross-reactivity with anti-dsDNA and anti-endothelial cell antibodies (AECA). In addition, it is also suggested that anti-P might play a role in the development of lupus nephritis through induction of T helper 1 responses. Of note, recent studies have disclosed that the presence of isolated anti-P antibodies may discriminate patients with pure class V lupus nephritis, whereas the simultaneous presence of anti-dsDNA antibodies suggests class V disease with concomitant proliferative lesions. These observations lead to the hypothesis that anti-P and anti-dsDNA might result in membraneous changes and proliferative changes, respectively, although further investigation is required for confirmation.

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“…The observed pattern may provide preliminary information on the nature of the autoantibody in a given sample and even indicate which additional and specific tests should be conducted. Some examples are the following: nuclear speckled pattern, which suggests the presence of anti-Sm and/or anti-U1RNP (14) ; nucleolar pattern with perichromosome plate, which indicates anti fibrillarin antibodies (12) ; cytoplasmic dense fine speckled pattern, often associated with the presence of ribosomal P protein antibodies (9) ; centromeric nuclear pattern, which results from the identification of centromere proteins (CENPs) (6) ; nuclear type and fine speckled nucleolar with cytoplasmic staining and presence of fluorescent spots on the chromosome plate (mixed pattern), which is strongly associated with the presence of deoxyribonucleic acid (DNA) topoisomerase 1 antibodies (4) . Successive methodological improvements, namely the quality of secondary antibody and overall microscope resolution, have resulted in a significant increase in assay sensitivity, hence unexpected positive results have commonly been observed (11,17) .…”

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confidence: 99%

Variability in the recognition of distinctive immunofluorescence patterns in different brands of HEp-2 cell slides

Dellavance

Cruvinel²,

Francescantônio³

et al. 2013

J. Bras. Patol. Med. Lab.

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Introduction: Indirect immunofluorescence on HEp-2 cells is considered the gold standard for the detection of autoantibodies against cellular antigens. However, the culture conditions, cell fixation and permeabilization processes interfere directly in the preservation and spatial distribution of antigens. Therefore, one can assume that certain peculiarities in the processing of cellular substrate may affect the recognition of indirect immunofluorescence patterns associated with several autoantibodies. Objective: To evaluate a panel of serum samples representing nuclear, nucleolar, cytoplasmic, mitotic apparatus, and chromosome plate patterns on HEp-2 cell substrates from different suppliers. Materials and methods: Seven blinded observers, independent from the three selected reference centers, evaluated 17 samples yielding different nuclear, nucleolar, cytoplasmic and mitotic apparatus patterns on HEp-2 cell slides from eight different brands. The slides were coded to maintain confidentiality of both brands and participating centers. Results: The 17 HEp-2 cell patterns were identified on most substrates. Nonetheless, some slides showed deficit in the expression of several patterns: nuclear coarse speckled/U1-ribonucleoprotein associated with antibodies against RNP (U1RNP), centromeric protein F (CENP-F), proliferating cell nuclear antigen (PCNA), cytoplasmic fine speckled associated with anti-Jo-1 antibodies (histidyl synthetase), nuclear mitotic apparatus protein 1 (NuMA-1) and nuclear mitotic apparatus protein 2 (NuMA-2). Conclusion: Despite the overall good quality of the assessed HEp-2 substrates, there was considerable inconsistency in results among different commercial substrates. The variations may be due to the evaluated batches, hence generalizations cannot be made as to the respective brands. It is recommended that each new batch or new brand be tested with a panel of reference sera representing the various patterns.

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Clinical and diagnostic value of ribosomal P autoantibodies in systemic lupus erythematosus

Cited by 44 publications

References 38 publications

Multi-center evaluation of autoantibodies to the major ribosomal P C22 epitope

Multi-center evaluation of autoantibodies to the major ribosomal P C22 epitope

Anti-ribosomal P antibodies and lupus nephritis

Variability in the recognition of distinctive immunofluorescence patterns in different brands of HEp-2 cell slides

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