Clinical and Diagnostic Developments of a Gamma Interferon Release Assay for Use in Bovine Tuberculosis Control Programs

Bass, K. E.; Nonnecke, Brian J.; Palmer, Mitchell V.; Thacker, Tyler C.; Hardegger, Roland; Schroeder, Bjoern O.; Raeber, Alex J.; Waters, W. Ray

doi:10.1128/cvi.00519-13

Cited by 30 publications

(17 citation statements)

References 30 publications

(39 reference statements)

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“…bovis 10–7428 resulted in a similar distribution and severity of gross and microscopic tuberculous lesions as well as mycobacterial colonization, primarily affecting the lungs and lung-associated lymph nodes [ 43 ]. Specific cell-mediated and antibody responses, including kinetics of the response as well as antigen recognition profiles, were also comparable between the two treatment groups [ 43 , 44 ].…”

Section: Resultsmentioning

confidence: 99%

Characterization of Effector and Memory T Cell Subsets in the Immune Response to Bovine Tuberculosis in Cattle

et al. 2015

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Cultured IFN-γ ELISPOT assays are primarily a measure of central memory T cell (Tcm) responses with humans; however, this important subset of lymphocytes is poorly characterized in cattle. Vaccine-elicited cultured IFN-γ ELISPOT responses correlate with protection against bovine tuberculosis in cattle. However, whether this assay measures cattle Tcm responses or not is uncertain. The objective of the present study was to characterize the relative contribution of Tcm (CCR7+, CD62Lhi, CD45RO+), T effector memory (Tem, defined as: CCR7-, CD62Llow/int, CD45RO+), and T effector cells (CCR7-, CD62L-/low, CD45RO-), in the immune response to Mycobacterium bovis. Peripheral blood mononuclear cells (PBMC) from infected cattle were stimulated with a cocktail of M. bovis purified protein derivative, rTb10.4 and rAg85A for 13 days with periodic addition of fresh media and rIL-2. On day 13, cultured PBMC were re-stimulated with medium alone, rESAT-6:CFP10 or PPDb with fresh autologous adherent cells for antigen presentation. Cultured cells (13 days) or fresh PBMCs (ex vivo response) from the same calves were analyzed for IFN-γ production, proliferation, and CD4, CD45RO, CD62L, CD44, and CCR7 expression via flow cytometry after overnight stimulation. In response to mycobacterial antigens, ~75% of CD4+ IFN-γ+ cells in long-term cultures expressed a Tcm phenotype while less than 10% of the ex vivo response consisted of Tcm cells. Upon re-exposure to antigen, long-term cultured cells were highly proliferative, a distinctive characteristic of Tcm, and the predominant phenotype within the long-term cultures switched from Tcm to Tem. These findings suggest that proliferative responses of Tcm cells to some extent occurs simultaneously with reversion to effector phenotypes (mostly Tem). The present study characterizes Tcm cells of cattle and their participation in the response to M. bovis infection.

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Section: Resultsmentioning

confidence: 99%

Characterization of Effector and Memory T Cell Subsets in the Immune Response to Bovine Tuberculosis in Cattle

et al. 2015

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“…The response to M. bovis PPD by strain 10-7428-infected calves exceeded (P Ͻ 0.05) the response by 95-1315-infected calves at 2 weeks after challenge, whereas responses to rESAT-6:CFP10 and M. bovis PPD by 95-1315-infected calves exceeded (P Ͻ 0.05) the responses by 10-7428-infected calves at 8 weeks after challenge. As with IFN-␥ responses (40), IL-17A responses to M. bovis PPD generally exceeded the responses to M. avium PPD (Fig. 2), and the responses to PPDs from two commercial sources were similar (M. avium PPD, CSL versus Lelystad, Spearman's ϭ 0.69; M. bovis PPD, CSL versus Lelystad, ϭ 0.82).…”

Section: Il-17a Responses To M Bovis Infection and Comparisons With mentioning

confidence: 99%

Interleukin-17A as a Biomarker for Bovine Tuberculosis

Waters

Maggioli

Palmer

et al. 2016

Clin Vaccine Immunol

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“…tuberculosis , i.e. early secretory antigenic target 6 kDa (ESAT-6) and culture filtrate protein 10 kDa (CFP-10), have been incorporated into IGRAs [ 3 ]. However, the sensitivity of these highly specific tests is lower than those using PPDb and PPDa [ 2 , 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Antigen-Specific IP-10 Release Is a Sensitive Biomarker of Mycobacterium bovis Infection in Cattle

et al. 2016

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The most widely used ante-mortem diagnostic tests for tuberculosis in cattle are the tuberculin skin test and the interferon-gamma (IFN-γ) release assay, both of which measure cell-mediated immune responses to Mycobacterium bovis infection. However, limitations in the performance of these tests results in a failure to identify all infected animals. In attempting to increase the range of diagnostic tests for tuberculosis, measurement of the cytokine IP-10 in antigen-stimulated blood has previously been shown to improve the detection of M. tuberculosis and M. bovis infection, in humans and African buffaloes (Syncerus caffer), respectively. In the present study, 60 cattle were identified by the single intradermal comparative tuberculin test as tuberculosis reactors (n = 24) or non-reactors (n = 36) and the release of IFN-γ and IP-10 in antigen-stimulated whole blood from these animals was measured using bovine specific ELISAs. There was a strong correlation between IP-10 and IFN-γ production in these samples. Moreover, measurement of the differential release of IP-10 in response to stimulation with M. bovis purified protein derivative (PPD) and M. avium PPD distinguished between reactor and non-reactor cattle with a sensitivity of 100% (95% CI, 86%–100%) and a specificity of 97% (95% CI, 85%–100%). These results suggest that IP-10 might prove valuable as a diagnostic biomarker of M. bovis infection in cattle.

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Clinical and Diagnostic Developments of a Gamma Interferon Release Assay for Use in Bovine Tuberculosis Control Programs

Cited by 30 publications

References 30 publications

Characterization of Effector and Memory T Cell Subsets in the Immune Response to Bovine Tuberculosis in Cattle

Characterization of Effector and Memory T Cell Subsets in the Immune Response to Bovine Tuberculosis in Cattle

Interleukin-17A as a Biomarker for Bovine Tuberculosis

Antigen-Specific IP-10 Release Is a Sensitive Biomarker of Mycobacterium bovis Infection in Cattle

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