Clinical Accuracy of a PLEX-ID Flu Device for Simultaneous Detection and Identification of Influenza Viruses A and B

Abstract

Respiratory tract infections caused by influenza A and B viruses often present nonspecifically, and a rapid, high-throughput laboratory technique that can identify influenza viruses is

Influenza in the US Military: An Overview

“…Among adult patients with ARI in one study using this type of testing in the United States in 2012-2013, 5% to 8% were found to sustain viral coinfections, to include influenza, HCoVs, RSV and HRV [35]. One influenza typing kit based on the RT-PCR electrospray ionization mass spectrometry (PCR-ESI-MS) platforms allows detection of all 16 hemagglutinin and 9 neuraminidase subtypes, [36] as well as detection of drift of specific genes over time [36][37][38][39]. Because of its ability to detect newly emerging recombinant, drifted, or shifted influenza viruses, the PCR-ESI-MS typing analysis can be useful in detecting newly emerging influenza strains [40].…”

Present and Future Applications of High Resolution Mass Spectrometry in the Clinic

“…With carefully designed sequence primers, subtyping has become possible. Tang et al reported on the accuracy of the influenza A and B assay, PLEX-ID flu 29 . They found that the overall accuracy when evaluating the presence of either influenza A or B and if found to be influenza A subtyped as H1N1-p, H1N1-s, or H3N2 compared to a RT-PCR based assay Prodesse ProFLU+ for Influenza A or B typing, and Prodesse ProFAST+ for A subtyping to be 97.1-100%.…”

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

“…These procedures are troublesome, however, fully automated analytical systems have been developed commercially involving polymerase chain reaction (PCR), sample preparation and ESI-MS for the detection of pathogens [15][16][17][18][19][20] . Another approach is introducing liquid chromatography (LC) for separation.…”

“…Alternatively, Muddiman et al developed a software application to calculate the base composition from the accurate mass 43 . A similar program was integrated into the automated ESI-MS system [16][17][18] . The use of these algorithms may improve the robustness of the present method because a measured monoisotopic mass does not always correspond to a unique base composition owing to the mass tolerance of 3 ppm resulting from the inevitable measurement error.…”

“…As for the detection of DNA polymorphism, the dedicated ESI-MS system is fully automated and easy to operate, which may be suitable for diagnostic use such as detection of pathogens 15,17,18,20,30 . Conversely, the present method is feasible with common research instruments and apparatus and, therefore, suitable for research uses.…”