Climbing the Branches of the Strigolactones Pathway One Discovery at a Time

Goulet, Charles; Klee, Harry J.

doi:10.1104/pp.110.161026

Cited by 15 publications

(12 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mutants in SL biosynthesis and signaling increase branching in diverse plant species, including Arabidopsis, pea, and petunia (for review, see Goulet and Klee, 2010), as well as tomato (Solanum lycopersicum; Vogel et al, 2010), kiwifruit (Actinidia deliciosa; Ledger et al, 2010), and rice (Wang and Li, 2011). In contrast with the prevailing model, which places SLs upstream of Tb1 orthologs in a linear pathway of branching control, our results indicate that Tb1 mediates a branchingspecific subnetwork that has become independent of SL signaling in maize.…”

Section: Discussioncontrasting

confidence: 52%

“…Key constituents of the SL biosynthetic pathway and signaling network have emerged through analysis of branching mutants in multiple plant species (Goulet and Klee, 2010). These include the more axillary growth (max) mutants of Arabidopsis (Arabidopsis thaliana; Sorefan et al, 2003;Booker et al, 2004), ramosus (rms) mutants of pea (Pisum sativum; Foo et al, 2005), decreased apical dominance (dad) mutants of petunia (Petunia hybrida; Snowden et al, 2005;Drummond et al, 2009), and the dwarf (d) and high tillering dwarf (htd) mutants of rice (Oryza sativa; Zou et al, 2006;Arite et al, 2007).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Diverse Roles of Strigolactone Signaling in Maize Architecture and the Uncoupling of a Branching-Specific Subnetwork

et al. 2012

Self Cite

View full text Add to dashboard Cite

Strigolactones (SLs) control lateral branching in diverse species by regulating transcription factors orthologous to Teosinte branched1 (Tb1). In maize (Zea mays), however, selection for a strong central stalk during domestication is attributed primarily to the Tb1 locus, leaving the architectural roles of SLs unclear. To determine how this signaling network is altered in maize, we first examined effects of a knockout mutation in an essential SL biosynthetic gene that encodes CAROTENOID CLEAVAGE DIOXYGENASE8 (CCD8), then tested interactions between SL signaling and Tb1. Comparative genome analysis revealed that maize depends on a single CCD8 gene (ZmCCD8), unlike other panicoid grasses that have multiple CCD8 paralogs. Function of ZmCCD8 was confirmed by transgenic complementation of Arabidopsis (Arabidopsis thaliana) max4 (ccd8) and by phenotypic rescue of the maize mutant (zmccd8::Ds) using a synthetic SL (GR24). Analysis of the zmccd8 mutant revealed a modest increase in branching that contrasted with prominent pleiotropic changes that include (1) marked reduction in stem diameter, (2) reduced elongation of internodes (independent of carbon supply), and (3) a pronounced delay in development of the centrally important, nodal system of adventitious roots. Analysis of the tb1 zmccd8 double mutant revealed that Tb1 functions in an SL-independent subnetwork that is not required for the other diverse roles of SL in development. Our findings indicate that in maize, uncoupling of the Tb1 subnetwork from SL signaling has profoundly altered the balance between conserved roles of SLs in branching and diverse aspects of plant architecture.

show abstract

Section: Discussioncontrasting

confidence: 52%

mentioning

confidence: 99%

Diverse Roles of Strigolactone Signaling in Maize Architecture and the Uncoupling of a Branching-Specific Subnetwork

et al. 2012

Self Cite

View full text Add to dashboard Cite

show abstract

“…Also is to consider the newly identified plant hormone strigolactones derived from carotenoids [45,47]. Several genes involved in their biosynthesis have been identified but knowledge of both the biosynthesis pathway and the transport of strigolactones and their intermediates is still incomplete [53]. How some highly hydrophobic intermediates such as carotenoid-derived compound can be transported within the cytoplasm of the cell or on more long distance?…”

Section: Discussionmentioning

confidence: 99%

Integrating genome annotation and QTL position to identify candidate genes for productivity, architecture and water-use efficiency in Populus spp

Monclus

Leplé²,

Bastien³

et al. 2012

BMC Plant Biol

View full text Add to dashboard Cite

BackgroundHybrid poplars species are candidates for biomass production but breeding efforts are needed to combine productivity and water use efficiency in improved cultivars. The understanding of the genetic architecture of growth in poplar by a Quantitative Trait Loci (QTL) approach can help us to elucidate the molecular basis of such integrative traits but identifying candidate genes underlying these QTLs remains difficult. Nevertheless, the increase of genomic information together with the accessibility to a reference genome sequence (Populus trichocarpa Nisqually-1) allow to bridge QTL information on genetic maps and physical location of candidate genes on the genome. The objective of the study is to identify QTLs controlling productivity, architecture and leaf traits in a P. deltoides x P. trichocarpa F1 progeny and to identify candidate genes underlying QTLs based on the anchoring of genetic maps on the genome and the gene ontology information linked to genome annotation. The strategy to explore genome annotation was to use Gene Ontology enrichment tools to test if some functional categories are statistically over-represented in QTL regions.ResultsFour leaf traits and 7 growth traits were measured on 330 F1 P. deltoides x P. trichocarpa progeny. A total of 77 QTLs controlling 11 traits were identified explaining from 1.8 to 17.2% of the variation of traits. For 58 QTLs, confidence intervals could be projected on the genome. An extended functional annotation was built based on data retrieved from the plant genome database Phytozome and from an inference of function using homology between Populus and the model plant Arabidopsis. Genes located within QTL confidence intervals were retrieved and enrichments in gene ontology (GO) terms were determined using different methods. Significant enrichments were found for all traits. Particularly relevant biological processes GO terms were identified for QTLs controlling number of sylleptic branches: intervals were enriched in GO terms of biological process like ‘ripening’ and ‘adventitious roots development’.ConclusionBeyond the simple identification of QTLs, this study is the first to use a global approach of GO terms enrichment analysis to fully explore gene function under QTLs confidence intervals in plants. This global approach may lead to identification of new candidate genes for traits of interest.

show abstract

“…Next, we examined the β-carotene branch of the pathway, from which ABA and strigolactones are derived. These apocarotenoids are phytohormones with roles in environmental response and development (21)(22)(23)(24)(25)(51)(52)(53)(54)(55)(56)(57)(58)(59)(60)(61). Production of apocarotenoids, including ABA and strigolactone, requires oxidative cleavage of carotenoid precursors.…”

Section: Resultsmentioning

confidence: 99%

“…For example, carotenoid pigments and volatile apocarotenoids, such as α-and β-ionone, are key aroma and flavor elements in attracting the agents of pollination and seed dispersal (18)(19)(20). Carotenoids serve as precursors for abscisic acid (ABA) and strigolactones, phytohormones that function in plant development as well as in response to the environment (21)(22)(23)(24)(25), and have also been implicated in the production of other regulatory signaling molecules (26,27). Despite diverse roles in plant growth and development, functional analyses of carotenoids have largely been focused on aboveground organs.…”

Section: Significancementioning

confidence: 99%

Periodic root branching in Arabidopsis requires synthesis of an uncharacterized carotenoid derivative

Norman

Zhang

Cazzonelli

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

109

117

View full text Add to dashboard Cite

In plants, continuous formation of lateral roots (LRs) facilitates efficient exploration of the soil environment. Roots can maximize developmental capacity in variable environmental conditions through establishment of sites competent to form LRs. This LR prepattern is established by a periodic oscillation in gene expression near the root tip. The spatial distribution of competent (prebranch) sites results from the interplay between this periodic process and primary root growth; yet, much about this oscillatory process and the formation of prebranch sites remains unknown. We find that disruption of carotenoid biosynthesis results in seedlings with very few LRs. Carotenoids are further required for the output of the LR clock because inhibition of carotenoid synthesis also results in fewer sites competent to form LRs. Genetic analyses and a carotenoid cleavage inhibitor indicate that an apocarotenoid, distinct from abscisic acid or strigolactone, is specifically required for LR formation. Expression of a key carotenoid biosynthesis gene occurs in a spatially specific pattern along the root's axis, suggesting spatial regulation of carotenoid synthesis. These results indicate that developmental prepatterning of LRs requires an uncharacterized carotenoid-derived molecule. We propose that this molecule functions non-cell-autonomously in establishment of the LR prepattern.root development | patterning | secondary metabolite synthesis A nchorage and uptake of water and soluble nutrients are essential functions of plant root systems and key to plant productivity and survival. The capacity of a root system to carry out these functions can be maximized by iterative root branching. Root branches are formed de novo during primary root growth, which allows for the elaboration of a complex root system that effectively enables the plant to navigate and exploit the resources of diverse, locally variable subterranean environments. Understanding the developmental mechanisms underlying the pattern of root branches has broad significance in both basic and applied research.As with other dicotyledonous plants, formation of a complex root system in the model plant Arabidopsis thaliana (Arabidopsis) occurs through iterative production of branches, or lateral roots (LRs), from the primary root. The simplified root system of Arabidopsis has yielded considerable insight into the cellular events and molecular regulators required for LR formation (recently reviewed in refs.

show abstract

Climbing the Branches of the Strigolactones Pathway One Discovery at a Time

Cited by 15 publications

References 22 publications

Diverse Roles of Strigolactone Signaling in Maize Architecture and the Uncoupling of a Branching-Specific Subnetwork

Diverse Roles of Strigolactone Signaling in Maize Architecture and the Uncoupling of a Branching-Specific Subnetwork

Integrating genome annotation and QTL position to identify candidate genes for productivity, architecture and water-use efficiency in Populus spp

Periodic root branching in Arabidopsis requires synthesis of an uncharacterized carotenoid derivative

Contact Info

Product

Resources

About