Erosion is a worldwide threat to biodiversity conservation and agricultural yield, and it is linked to deforestation. In this study, we aim to assess soil loss in landscapes of Cachoeira River watershed, in southern Bahia, northeastern Brazil. We estimate the role of forests in diminishing soil erosion using the Revised Universal Soil Loss Equation (RUSLE). We compare real and simulated scenarios in which the forest was replaced by agricultural use, also comparing estimates of erosivity factor (R factor) derived from remote sensing and climatological station data. Real and simulated annual soil losses varied from 0 to 15.95 t/year and from 0 to 33.53 t/year along the watershed, respectively. However, only 0.04 and 1.67% of this area is highly and severely exposed to erosion, using data from climatological stations and remote sensing, respectively. Soil loss in the simulated deforested scenario was approximately two times higher than the real annual soil loss, indicating the importance of forest cover to mitigate soil erosion. Moreover, soil loss was 10.5 times greater when using precipitation data from remote sensing compared to climatological stations. Conclusively, the practice of agroforestry can be used as an alternative to avoid erosion.