Dengue fever is a virus-borne disease spread by mosquitos, and its global prevalence has risen significantly in recent years. The aim of this study was to analyze the impact and association of climatic factors on the spread of dengue incidence in Bangladesh. From January 2011 to December 2021, the study used secondary data on monthly dengue cases and the monthly average of climatic factors. In addition to the descriptive statistics, bivariate analyses of Kendall’s tau b and Spearman’s rho have been performed for measuring the association of climatic factors on dengue infection. The multivariate generalized linear negative binomial regression model was applied to evaluate the impacts of climatic factors on dengue transmission. The model revealed that temperature (OR: 152.745,p<0.01), humidity (OR: 1.15, p<0.01), precipitation (OR: 1.131, p<0.01), wind speed (OR: 1.148, p<0.10), and air pressure (OR: 152.745, p<0.01) were significantly influenced the spread of dengue incidence in Bangladesh. The climatic season also significantly influenced dengue transmission, and a higher infection rate (OR: 140.704, p<0.01) was found in the monsoon season at August month. The findings of this study can assist policymakers and public health authorities in taking the essential steps to control the dengue infection in Bangladesh.