The cultural layer (CL) is a layer of soil at the sites of human settlements that contains traces or remnants of human activity. From the point of view of geochemistry, the CL is a complex object of study. For a long time, impurities that are not characteristic of the natural soil come to be here, such as hair, nails, ceramics, ash, leather, fabric, wool, bones, manure, straw, coal, and food. The penetration of these objects into the soil leads to an increase in the content of biogenic elements: nitrogen, carbon, phosphorus, sulfur, calcium, iron, and a decrease in the proportion of elements, which mainly consists of the mineral matrix of the soil: silicon, aluminum, and potassium. As a result, the CL acquires properties different from those of the soil. It increases the weight loss on ignition and decreases the proportion of the solid phase, which creates significant difficulties for X-ray fluorescence analysis.