Clientism and Loyalty

Cu loading alters expression of non-IRE regulated, but not IRE regulated, Fe dependent proteins in HepG2 cells

“…Experiments were performed on 90% confluent cells of passages between 10 and 20. Desferrioxamine (DFO; final concentration 100 lM) or CuHis 2 (final concentration 20 lM) were used to induce Fe deficiency and Cu loading, respectively by incubating cells for 18 h. DNA content was quantified as previously described [20].…”

“…These experiments were carried out as previously described [20,21]. Cells were washed 3Â with BSS ice-cold.…”