Click Reaction-Mediated Fluorescent Immunosensor Based on Cu-MOF Nanoparticles for Ultrasensitive and High-Throughput Detection of Aflatoxin B1 in Food Samples
Feng Hong,
Yongkun Zhao,
Shixing Pan
et al.
Abstract:Due to the high toxicity of aflatoxin B 1 and its risks to human health, we developed a click reaction-mediated automated fluorescent immunosensor (CAFI) for sensitive detection of aflatoxin B 1 based on the Cu(I)-catalyzed click reaction. With its large specific surface area, a copper-based metal−organic framework (Cu-MOF) was synthesized to adsorb and enrich the copper ion (Cu(II)) and then load the complete antigen (BSA-AFB 1 ). After the immunoreaction, Cu(II) inside the Cu-MOF-Antigen conjugate would be r… Show more
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