“Click” Immobilization of a VEGF-Mimetic Peptide on Decellularized Endothelial Extracellular Matrix to Enhance Angiogenesis

Wang, Lin; Zhao, Meirong; Li, Siheng; Erasquin, Uriel J.; Wang, Hao; Li, Ren; Chen, Changyi; Wang, Yingjun; Cai, Chengzhi

doi:10.1021/am501309d

Cited by 39 publications

(37 citation statements)

References 40 publications

(95 reference statements)

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“…For this reason, QK has high affinity to bind to VEGF receptors on the surface of endothelial cells [38,116]. Many studies have revealed that peptide QK promotes endothelial cell migration, viability, and proliferation in vitro [129][130][131][132][133] as well as supports angiogenesis in vivo [130,133,134].…”

Section: Peptide Qkmentioning

confidence: 99%

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

2020

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Polymer scaffolds constitute a very interesting strategy for tissue engineering. Even though they are generally non-toxic, in some cases, they may not provide suitable support for cell adhesion, proliferation, and differentiation, which decelerates tissue regeneration. To improve biological properties, scaffolds are frequently enriched with bioactive molecules, inter alia extracellular matrix proteins, adhesive peptides, growth factors, hormones, and cytokines. Although there are many papers describing synthesis and properties of polymer scaffolds enriched with proteins or peptides, few reviews comprehensively summarize these bioactive molecules. Thus, this review presents the current knowledge about the most important proteins and peptides used for modification of polymer scaffolds for tissue engineering. This paper also describes the influence of addition of proteins and peptides on physicochemical, mechanical, and biological properties of polymer scaffolds. Moreover, this article sums up the major applications of some biodegradable natural and synthetic polymer scaffolds modified with proteins and peptides, which have been developed within the past five years.

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Section: Peptide Qkmentioning

confidence: 99%

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

2020

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“…16 The role of other ECM- and GF-derived peptides for directing cell behavior for vascularization are still being defined, including the use of a peptide that mimics vascular endothelial growth factor to direct angiogenesis. 9, 58, 60, 119 Also, peptide-driven self-assembly/gelation has been proposed as an improved bioink for organotypic culture and shown to be effective for in vivo angiogenesis. 17, 61-64, 79 Based on current trends, peptide modified polymers can be envisioned as a future direction for programming cell-specific bioink in 3D constructs to direct cell behavior for vascular patterning.…”

Section: Perspective and Conclusionmentioning

confidence: 99%

3D Bioprinting for Vascularized Tissue Fabrication

et al. 2016

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3D bioprinting holds remarkable promise for rapid fabrication of 3D tissue engineering constructs. Given its scalability, reproducibility, and precise multi-dimensional control that traditional fabrication methods do not provide, 3D bioprinting provides a powerful means to address one of the major challenges in tissue engineering: vascularization. Moderate success of current tissue engineering strategies have been attributed to the current inability to fabricate thick tissue engineering constructs that contain endogenous, engineered vasculature or nutrient channels that can integrate with the host tissue. Successful fabrication of a vascularized tissue construct requires synergy between high throughput, high-resolution bioprinting of larger perfusable channels and instructive bioink that promotes angiogenic sprouting and neovascularization. This review aims to cover the recent progress in the field of 3D bioprinting of vascularized tissues. It will cover the methods of bioprinting vascularized constructs, bioink for vascularization, and perspectives on recent innovations in 3D printing and biomaterials for the next generation of 3D bioprinting for vascularized tissue fabrication.

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“…The tube formation assay is a widely-applied tool to detect cell angiogenesis [6,47]. Matrigel (BD Biosciences, USA) was added in the 96-well plates and allowed to polymerize for 30 min at 37℃.…”

Section: Tube Formation Assaymentioning

confidence: 99%

Improvement of cardiac dysfunction by L-arginine combined with vitamin C through restoring angiogenesis associated with NADPH/NOS/NO signal pathway in diabetic rats

Cai

et al. 2020

Preprint

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Background In diabetic patients, the occurrence of ischemic events such as myocardial infarction is significantly higher than that of normal patients. L-arginine and vitamin C are commonly used adjuvant drugs in clinical practice. But whether L-arginine and vitamin C can be used together, the role of promoting angiogenesis to improve vascular dysfunction and its mechanism of action are not clear. To this end, we investigated whether L-arginine in combination with vitamin C promotes revascularization after myocardial infarction in patients with type 2 diabetes by inhibiting angiogenesis disorders associated with NADPH oxidase. Methods Experimental myocardial infarction (MI) was induced by left anterior descending coronary artery ligation in diabetic Sprague-Dawley rats and non-diabetic controls. Cardiac function was studied by echocardiography and LDH activity. Western blotting was used to study VEGF, Nox4, Nox1, Nox2, eNOS and phospho-eNOS (Ser1177), CAT-1. Angiogenesis, cell proliferation and migration were detected by micro-vessel density assay, cell viability assay, scratch wound-healing assay and tube formation assay. Results First, we cultured rat aortic endothelial cells in a high glucose environment for 48 hours, then treated L-arginine and vitamin C alone or together. It was shown that high glucose significantly damaged cell proliferation, migration and tube formation, which was normalized by L-arginine with vitamin C combined application. Furthermore, we found that co-treatment of L-arginine and vitamin C activated NOS/NO signaling pathway via inhibited NADPH oxidase activity, which regulated angiogenesis in RAECs. In vivo, L-arginine and vitamin C co-intervention improved cardiac function by implementing echocardiographic examination and LDH activity. This therapy also relieved the vasodilatory response to acetylcholine and restored the expressions of p-eNOS and VEGF, together with reducing Nox2 and iNOS production in the ischemic myocardium of high-fat diet (HFD)-fed rats following streptozocin (STZ) injection. Conclusion Overall, for the first time, this study reveals that the combination of L-arginine and vitamin C restores the blood supply to myocardial infarction in rats with type 2 diabetes by promoting angiogenesis, which was attributed to activating NOS/NO signaling pathway via blocking NADPH-dependent O2− generation.

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“Click” Immobilization of a VEGF-Mimetic Peptide on Decellularized Endothelial Extracellular Matrix to Enhance Angiogenesis

Cited by 39 publications

References 40 publications

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

3D Bioprinting for Vascularized Tissue Fabrication

Improvement of cardiac dysfunction by L-arginine combined with vitamin C through restoring angiogenesis associated with NADPH/NOS/NO signal pathway in diabetic rats

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