Click-Functionalized Compact Quantum Dots Protected by Multidentate-Imidazole Ligands: Conjugation-Ready Nanotags for Living-Virus Labeling and Imaging

Zhang, Pengfei; Liu, Shuhui; Gao, Duyang; Hu, Dehong; Gong, Ping; Sheng, Zonghai; Deng, Jizhe; Ma, Yifan; Cai, Lintao

doi:10.1021/ja302367s

Cited by 130 publications

(134 citation statements)

References 41 publications

Supporting

Mentioning

131

Contrasting

Order By: Relevance

“…Liu and co-workers used a polyhistidine-and azide-modified block copolymer, starting from polymaleic anhydride, to cap luminescent QDs. 145 They showed that following ligand exchange the azide groups were accessible for further conjugation to the Baculovirus pre-modified with cyclooctyne through metal-free ''Click'' reaction. These conjugates were tested in the intracellular uptake by the A549 cell line.…”

Section: Bioconjugation To Target Moleculesmentioning

confidence: 99%

Strategies for interfacing inorganic nanocrystals with biological systems based on polymer-coating

et al. 2015

View full text Add to dashboard Cite

Interfacing inorganic nanoparticles and biological systems with the aim of developing novel imaging and sensing platforms has generated great interest and much activity. However, the effectiveness of this approach hinges on the ability of the surface ligands to promote water-dispersion of the nanoparticles with long term colloidal stability in buffer media. These surface ligands protect the nanostructures from the harsh biological environment, while allowing coupling to target molecules, which can be biological in nature (e.g., proteins and peptides) or exhibit specific photo-physical characteristics (e.g., a dye or a redox-active molecule). Amphiphilic block polymers have provided researchers with versatile molecular platforms with tunable size, composition and chemical properties. Hence, several groups have developed a wide range of polymers as ligands or micelle capsules to promote the transfer of a variety of inorganic nanomaterials to buffer media (including magnetic nanoparticles and semiconductor nanocrystals) and render them biocompatible. In this review, we first summarize the established synthetic routes to grow high quality nanocrystals of semiconductors, metals and metal oxides. We then provide a critical evaluation of the recent developments in the design, optimization and use of various amphiphilic copolymers to surface functionalize the above nanocrystals, along with the strategies used to conjugate them to target biomolecules. We finally conclude by providing a summary of the most promising applications of these polymer-coated inorganic platforms in sensor design, and imaging of cells and tissues.

show abstract

Section: Bioconjugation To Target Moleculesmentioning

confidence: 99%

Strategies for interfacing inorganic nanocrystals with biological systems based on polymer-coating

et al. 2015

View full text Add to dashboard Cite

show abstract

“…However, in these experiments, no CLEM was performed. Zhang et al [38] recently used a similar approach to image the intracellular presence of viruses. CdSe/ZnS Qdots were modified with an azide-containing outer coating.…”

Section: Bioorthogonal Labelling For Clem Imagingmentioning

confidence: 99%

The potential of bioorthogonal chemistry for correlative light and electron microscopy: a call to arms

et al. 2015

View full text Add to dashboard Cite

With correlative light and electron microscopy (CLEM), the ultrastructural cellular location of a biomolecule of interest can be determined using a combination of light microscopy (LM) and electron microscopy (EM). In many cases, the application of CLEM requires the use of markers that need to be attached to a biomolecule of interest to allow its identification and localization. Here, we review the potential of bioorthogonal chemistry to introduce such markers for CLEM.

show abstract

“…The QDs are usually embedded in the matrixes to avoid their agglomeration, and in turn, the diverse structures of matrixes could widen the applications of QDs [4][5][6]. Polymers are most employed as matrixes for embedding the QDs due to their diversity, easy handing, and mechanical and chemical stability [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Simple synthesis of high-quality CdTe QDs in spherical polyelectrolyte brushes with stable and reversible photoluminescence

et al. 2015

View full text Add to dashboard Cite

A facile method of high-quality CdTe quantum dots (QDs) synthesis in spherical polyelectrolyte brushes (SPBs) was reported in this work. The size of CdTe QDs, with emission color from green to red, could be tuned by adjusting the duration of nucleation, feed ratio of reductants (NaBH 4 / N 2 H 4 ), and pH of precursor (SPB solution). Short duration of nucleation and low ratio of NaBH 4 /N 2 H 4 could promote the growth rate of QDs to form big-sized CdTe QDs. The pH of SPB solution, in the range of 7-9, was optimal condition for formation of high-quality QDs. The SPB@CdTe exhibited high photochemical stability as the pH of mixture was above 10, which could be stored in the visible light for several months without aggregation and optical deterioration. The photoluminescence emission of SPB@CdTe could be reversibly quenched and restored with shrinkage and swollen shell if employed pH as a regulator.

show abstract

Click-Functionalized Compact Quantum Dots Protected by Multidentate-Imidazole Ligands: Conjugation-Ready Nanotags for Living-Virus Labeling and Imaging

Cited by 130 publications

References 41 publications

Strategies for interfacing inorganic nanocrystals with biological systems based on polymer-coating

Strategies for interfacing inorganic nanocrystals with biological systems based on polymer-coating

The potential of bioorthogonal chemistry for correlative light and electron microscopy: a call to arms

Simple synthesis of high-quality CdTe QDs in spherical polyelectrolyte brushes with stable and reversible photoluminescence

Contact Info

Product

Resources

About