CLIC1 knockout inhibits invasion and migration of gastric cancer by upregulating AMOT-p130 expression

Qiu, Yuling; Mao, Ye-Bo; Zhu, Jinhui; Zhao, Kun; Wang, J.-f.; Huang, Jiegang; Chang, Geyun; Guan, Ying; Huang, Fuling; Hu, Yanling; Chen, Jinglian; Liu, J.-l.

doi:10.1007/s12094-020-02445-0

Cited by 10 publications

(9 citation statements)

References 24 publications

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“…In our study, CLIC1 was expressed with the highest score of 3 in all the LM cases, except one. In gastric cancer, the involvement of CLIC1 in metastatic progression was observed by reducing AMOT-p130 expression [34]. Scores of 2 and 3 in LM were found in the present study.…”

Section: Discussionsupporting

confidence: 61%

Chloride Intracellular Channel Protein 1 Expression and Angiogenic Profile of Liver Metastasis of Digestive Origin

Ceauşu

Ciolofan

Blidișel

et al. 2023

CIMB

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Chloride intracellular channel 1 (CLIC1) is involved in cell migration and metastasis. The histological growth patterns of liver metastasis are as follows: desmoplastic (d-HGP), replacement (r-HGP), pushing (p-HGP), and mixed. The aim of this study was to evaluate the relation between HGP, angiogenesis, and CLIC1 expression. Materials and Methods: A total of 40 cases of primary tumors and their LM: d-HGP (12 cases), r-HGP (13 cases), and p-HGP (15 cases), were evaluated through simple and double immunostaining. CLIC1 assessment was conducted as follows: scores of 0 (less than 10% of positive cells), 1 (10–30%), 2 (30–50%), or 3 (more than 50%) were assigned. Heterogeneous CLIC1 expression was found. CLIC1 in primary tumors correlated with grade G for all cases of LM with a p-HGP (p = 0.004). The CLIC1 score for LMs with an r-HGP correlated with grade G of the corresponding primary tumor (p = 0.027). CLIC1 and CD34+/Ki67+ vessels (p = 0.006) correlated in primary tumors. CLIC1 in primary tumors correlated with CD34+/Ki67+ vessels of LMs with a d HGP (p = 0.024). Conclusions: The CLIC1 score may have prognostic value, mainly for LMs with a p-HGP and r-HGP, and therapeutic value for LMs with a d-HGP.

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Section: Discussionsupporting

confidence: 61%

Chloride Intracellular Channel Protein 1 Expression and Angiogenic Profile of Liver Metastasis of Digestive Origin

Ceauşu

Ciolofan

Blidișel

et al. 2023

CIMB

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“…Qiu, Y. et al. found that CLIC1 knockout inhibits the invasion and migration of gastric cancer by upregulating AMOT-p130 expression ( 54 ). In cell proliferation, CLIC1 can auto-transmit from the cytoplasm to the plasma membrane without transport vesicles ( 55 ).…”

Section: Discussionmentioning

confidence: 99%

Label-free LC-MS/MS proteomics analyses reveal CLIC1 as a predictive biomarker for bladder cancer staging and prognosis

et al. 2023

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IntroductionBladder cancer (BC) is a significant carcinoma of the urinary system that has a high incidence of morbidity and death owing to the challenges in accurately identifying people with early-stage BC and the lack of effective treatment options for those with advanced BC. Thus, there is a need to define new markers of prognosis and prediction.MethodsIn this study, we have performed a comprehensive proteomics experiment by label-free quantitative proteomics to compare the proteome changes in the serum of normal people and bladder cancer patients—the successful quantification of 2064 Quantifiable proteins in total. A quantitative analysis was conducted to determine the extent of changes in protein species' relative intensity and reproducibility. There were 43 upregulated proteins and 36 downregulated proteins discovered in non-muscle invasive bladder cancer and normal individuals. Sixty-four of these proteins were elevated, and 51 were downregulated in muscle-invasive and non-muscle-invasive bladder cancer, respectively. Functional roles of differentially expressed proteins were annotated using Gene Ontology (GO) and Clusters of Orthologous Groups of Proteins (COG). To analyze the functions and pathways enriched by differentially expressed proteins, GO enrichment analysis, protein domain analysis, and KEGG pathway analysis were performed. The proteome differences were examined and visualized using radar plots, heat maps, bubble plots, and Venn diagrams.ResultsAs a result of combining the Venn diagram with protein-protein interactions (PPIs), Chloride intracellular channel 1 (CLIC1) was identified as the primary protein. Using the Gene Set Cancer Analysis (GSCA) website, the influence of CLIC1 on immune infiltration was analyzed. A negative correlation between CD8 naive and CLIC1 levels was found. For validation, immunohistochemical (IHC), qPCR, and western blotting (WB) were performed.Further, we found that CLIC1 was associated with a poor prognosis of bladder cancer in survival analysis.DiscussionOur research screened CLIC1 as a tumor-promoting protein in bladder cancer for the first time using serum mass spectrometry. And CLIC1 associated with tumor stage, and immune infiltrate. The prognostic biomarker and therapeutic target CLIC1 may be new for bladder cancer patients.

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“…In particular, the Ubiquitin C-terminal Hydrolase L1 (UCH-L1), a member of the deubiquitinase family involved in processing ubiquitin precursors and in regulating angiogenesis ( 63 ), and whose up-regulation has previously been demonstrated to enhance cell invasion/metastasis ( 64 , 65 ), and increase multidrug resistance in breast cancer ( 66 , 67 ), resulted highly over-expressed in PC, suggesting an oncogenic role of this protein in the malignant carcinogenesis of parathyroid tissue and, at the same time, indicating UCH-L1 as a possible distinctive immunohistochemical signature of PC with respect to benign parathyroid tumors. Other promising protein biomarkers, all over-expressed in PCs, were: Malate Dehydrogenase 1 (MDH1), an enzyme that catalyzes the NAD/NADH-dependent reversible oxidation of malate to oxaloacetate in many metabolic pathways and was showed to be up-regulated in cancer ( 68 ); Chloride Intracellular Channel Protein 1 (CLIC1), a protein involved in regulating cell proliferation and differentiation, and metastasis ( 69 , 70 ), and whose up-regulation has previously been associated with human malignancies; and Superoxide Dismutase 2 (SOD2), which is a key player of the antioxidant system of the cell and whose increased expression appears correlated to the stage of tumor progression ( 71 ).…”

Section: Gene Expression Profile and Proteomic Analysis In Parathyroi...mentioning

confidence: 99%

Genetics and Epigenetics of Parathyroid Carcinoma

et al. 2022

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Parathyroid carcinoma (PC) is an extremely rare malignancy, accounting less than 1% of all parathyroid neoplasms, and an uncommon cause of primary hyperparathyroidism (PHPT), characterized by an excessive secretion of parathyroid hormone (PTH) and severe hypercalcemia. As opposed to parathyroid hyperplasia and adenomas, PC is associated with a poor prognosis, due to a commonly unmanageable hypercalcemia, which accounts for death in the majority of cases, and an overall survival rate of 78-85% and 49-70% at 5 and 10 years after diagnosis, respectively. No definitively effective therapies for PC are currently available. The mainly employed treatment for PC is the surgical removal of tumoral gland(s). Post-surgical persistent or recurrent disease manifest in about 50% of patients. The comprehension of genetic and epigenetic bases and molecular pathways that characterize parathyroid carcinogenesis is important to distinguish malignant PCs from benign adenomas, and to identify specific targets for novel therapies. Germline heterozygote inactivating mutations of the CDC73 tumor suppressor gene, with somatic loss of heterozygosity at 1q31.2 locus, account for about 50-75% of familial cases; over 75% of sporadic PCs harbor biallelic somatic inactivation/loss of CDC73. Recurrent mutations of the PRUNE2 gene, a recurrent mutation in the ADCK1 gene, genetic amplification of the CCND1 gene, alterations of the PI3K/AKT/mTOR signaling pathway, and modifications of microRNA expression profile and gene promoter methylation pattern have all been detected in PC. Here, we review the current knowledge on gene mutations and epigenetic changes that have been associated with the development of PC, in both familial and sporadic forms of this malignancy.

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CLIC1 knockout inhibits invasion and migration of gastric cancer by upregulating AMOT-p130 expression

Cited by 10 publications

References 24 publications

Chloride Intracellular Channel Protein 1 Expression and Angiogenic Profile of Liver Metastasis of Digestive Origin

Chloride Intracellular Channel Protein 1 Expression and Angiogenic Profile of Liver Metastasis of Digestive Origin

Label-free LC-MS/MS proteomics analyses reveal CLIC1 as a predictive biomarker for bladder cancer staging and prognosis

Genetics and Epigenetics of Parathyroid Carcinoma

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