Cleaved Slit directs embryonic muscles

Ordan, Elly; Volk, Talila

doi:10.1080/19336934.2015.1102808

Cited by 4 publications

(5 citation statements)

References 20 publications

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“…In embryos expressing amon RNAi in the ectoderm the LT muscles were closer to the segmental border (Fig. 1C,D), similar to the phenotype observed in slit mutants (Ordan et al, 2015), robo,robo3 mutants (Ordan and Volk, 2015a,b), and homozygous amon mutant embryos (Fig. 1B).…”

Section: Resultssupporting

confidence: 78%

“…The ratio of D LT3 /D S was 57% smaller than in wild type, implying that the LT muscles were significantly closer to the segmental border compared with wild-type embryos ( P =0.001). Importantly, a 60% reduction in the ratio of D LT3 /D S was previously reported in slit mutants (Ordan et al, 2015), and a 61% reduction in that ratio was reported in robo;robo3 mutant muscles (Ordan and Volk, 2015a,b). These results are consistent with the involvement of Amon protease in Slit processing.…”

Section: Resultsmentioning

confidence: 51%

“…Based on a growing amount of data, it has been suggested that different muscle types exhibit an individual intrinsic polarity, independent of tendon signals, that governs their basic directionality (Krzemien et al, 2012; Ordan et al, 2015; Schnorrer and Dickson, 2004a). The external signals provided by tendons are of a short-range nature, and are necessary for fine-tuning the muscle path, either by repulsion or by providing a stop signal at the presumptive attachment site (Ordan and Volk, 2015a). An essential signal for directing muscle elongation is mediated by the secreted protein Slit.…”

Section: Introductionmentioning

confidence: 99%

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Amontillado is required forDrosophilaSlit processing and for tendon-mediated muscle patterning

Ordan

Volk

2016

Biology Open

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Slit cleavage into N-terminal and C-terminal polypeptides is essential for restricting the range of Slit activity. Although the Slit cleavage site has been characterized previously and is evolutionally conserved, the identity of the protease that cleaves Slit remains elusive. Our previous analysis indicated that Slit cleavage is essential to immobilize the active Slit-N at the tendon cell surfaces, mediating the arrest of muscle elongation. In an attempt to identify the protease required for Slit cleavage we performed an RNAi-based assay in the ectoderm and followed the process of elongation of the lateral transverse muscles toward tendon cells. The screen led to the identification of the Drosophila homolog of pheromone convertase 2 (PC2), Amontillado (Amon), as an essential protease for Slit cleavage. Further analysis indicated that Slit mobility on SDS polyacrylamide gel electrophoresis (SDS-PAGE) is slightly up-shifted in amon mutants, and its conventional cleavage into the Slit-N and Slit-C polypeptides is attenuated. Consistent with the requirement for amon to promote Slit cleavage and membrane immobilization of Slit-N, the muscle phenotype of amon mutant embryos was rescued by co-expressing a membrane-bound form of full-length Slit lacking the cleavage site and knocked into the slit locus. The identification of a novel protease component essential for Slit processing may represent an additional regulatory step in the Slit signaling pathway.

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Section: Resultssupporting

confidence: 78%

Section: Resultsmentioning

confidence: 51%

Section: Introductionmentioning

confidence: 99%

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Amontillado is required forDrosophilaSlit processing and for tendon-mediated muscle patterning

Ordan

Volk

2016

Biology Open

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“…20 In Drosophila, Slit acts as a long range chemorepellent to drive the migration of muscle precursor cells away from the midline, 5 and expression of Slit at epidermal muscle attachment sites arrests migration of muscle cells to facilitate correct development of muscle-tendon attachment sites. [21][22][23][24] Reflecting their roles during development of multiple tissues, Slits and Robos are expressed in regions of the developing vertebrate embryo outside the nervous system such as branchial arches, reproductive organs, developing heart and kidney and the limb bud of mouse and chicken embryos, particularly in and around the forming joints and muscle regions. 2,6,9,11,18,[25][26][27] Here we focus on the nature and role of Slit/Robo signaling during limb development.…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, Slit/Robo signaling can promote and inhibit endothelial cell migration and angiogenesis, 16‐19 and is required during heart development with Robo1/2 mutant mice presenting with partial absence of pericardium, reduced sinus horn myocardium and alignment defects of caval veins 20 . In Drosophila , Slit acts as a long range chemorepellent to drive the migration of muscle precursor cells away from the midline, 5 and expression of Slit at epidermal muscle attachment sites arrests migration of muscle cells to facilitate correct development of muscle‐tendon attachment sites 21‐24 …”

Section: Introductionmentioning

confidence: 99%

Knockdown of slit signaling during limb development leads to a reduction in humerus length

Rafipay

Dun

Parkinson

et al. 2021

Developmental Dynamics

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Background: Slits (1-3) and their Robo (1-3) receptors play multiple nonneuronal roles in development, including in development of muscle, heart and mammary gland. Previous work has demonstrated expression of Slit and Robo family members during limb development, where their functions are unclear. Results: In situ hybridisation confirmed strong expression of Slit2, Slit3, Robo1, and Robo2 throughout mouse limb and joint development. No expression of Slit1 or Robo3 was detected. Analysis of Slit1/2 or Slit3 knockout mice revealed normal limb development. In contrast, locally blocking Slit signaling though grafting of cells expressing a dominant-negative Robo2 construct in the proximo-central region of developing chicken limb buds caused significant shortening of the humerus. Conclusions: These findings demonstrate an essential role for Slit/Robo signaling in regulating bone length during chicken limb development.

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Robo signaling regulates the production of cranial neural crest cells

Zhang

Wang

et al. 2017

Experimental Cell Research

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Slit/Robo signaling plays an important role in the guidance of developing neurons in developing embryos. However, it remains obscure whether and how Slit/Robo signaling is involved in the production of cranial neural crest cells. In this study, we examined Robo1 deficient mice to reveal developmental defects of mouse cranial frontal and parietal bones, which are derivatives of cranial neural crest cells. Therefore, we determined the production of HNK1 cranial neural crest cells in early chick embryo development after knock-down (KD) of Robo1 expression. Detection of markers for pre-migratory and migratory neural crest cells, PAX7 and AP-2α, showed that production of both was affected by Robo1 KD. In addition, we found that the transcription factor slug is responsible for the aberrant delamination/EMT of cranial neural crest cells induced by Robo1 KD, which also led to elevated expression of E- and N-Cadherin. N-Cadherin expression was enhanced when blocking FGF signaling with dominant-negative FGFR1 in half of the neural tube. Taken together, we show that Slit/Robo signaling influences the delamination/EMT of cranial neural crest cells, which is required for cranial bone development.

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Cleaved Slit directs embryonic muscles

Cited by 4 publications

References 20 publications

Amontillado is required forDrosophilaSlit processing and for tendon-mediated muscle patterning

Amontillado is required forDrosophilaSlit processing and for tendon-mediated muscle patterning

Knockdown of slit signaling during limb development leads to a reduction in humerus length

Robo signaling regulates the production of cranial neural crest cells

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