Cleavage speed and implantation potential of early-cleavage embryos in IVF or ICSI cycles

Lee, Meng-Ju; Lee, Robert Kuo-Kuang; Lin, Ming-Huei; Hwu, Yuh‐Ming

doi:10.1007/s10815-012-9777-z

Cited by 43 publications

(32 citation statements)

References 18 publications

(36 reference statements)

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“…In fact, among factors known to influence the outcome of IVF/ICSI treatment, morphology and developmental speed are considered the most reasonable and common parameters as markers of embryo quality prior to uterine transfer. It has been recently shown that early onset-ofcleavage in human embryos is a predictor of normal development and high chance of live birth when compared with late onset-of-cleavage embryos in IVF/ICSI cycles [31]. Successful implantation of early cleaving embryos may be the result, among others, of a more optimal synchronization between embryonic development and the preparation of a receptive uterine environment [32].…”

Section: Discussionmentioning

confidence: 99%

Improvement of mouse embryo quality by myo-inositol supplementation of IVF media

Colazingari

Fiorenza

Carlomagno³

et al. 2014

J Assist Reprod Genet

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Objective Myo-inositol (myoIns) has a positive role in mammalian development and human reproduction. Since experiments on farming species suggest a similar role in preimplantation development, we evaluated the hypothesis that the inclusion of myoIns in human embryo culture media would produce an increase in embryo quality in IVF cycles, using the mouse embryo assay. Methods To determine the effect of myoIns on completion of preimplantation development in vitro, one-cell embryos of the inbred C57BL/6N mouse strain were produced by ICSI, cultured in human fertilization media in the presence of myoIns (myoIns+) or in its absence (myoIns-) and evaluated morphologically. Daily progression through cleavage stages, blastocyst production and expansion and blastomere number at 96 hours post fertilization were assessed. Results Compared to myoIns-embryos, myoIns+ embryos displayed a faster cleavage rate and by the end of preimplantation development, the majority of myoIns+ blastocysts was expanded and formed by a higher number of blastomeres. Conclusion The presence of myoIns resulted in both an increase in proliferation activity and developmental rate of in vitro cultured early mouse embryos, representing a substantial improvement of culture conditions. These data may identify myoIns as an important supplement for human embryo preimplantation culture.

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Section: Discussionmentioning

confidence: 99%

Improvement of mouse embryo quality by myo-inositol supplementation of IVF media

Colazingari

Fiorenza

Carlomagno³

et al. 2014

J Assist Reprod Genet

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“…For cleavage-stage embryos it has been reported that, the implantation rate is higher for embryos that cleave earlier (Lee et al, 2012). Similarly studies involving blastocyst transfer procedures have examined differences in implantation rates between blastocysts developing up until day 5 versus day 6 (Stehlik et al, 2005;Liebermann et al, 2006;Sunkara et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Women's age and embryo developmental speed accurately predict clinical pregnancy after single vitrified-warmed blastocyst transfer

Kato

Ueno²,

Yabuuchi³

et al. 2014

Reproductive BioMedicine Online

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The aim of this study is to establish a simple, objective blastocyst grading system using women's age and embryo developmental speed to predict clinical pregnancy following single vitrified-warmed blastocyst transfer (SVBT) by 6-year retrospective cohort study in a private infertility center.A total of 7,341 SVBT cycles divided into 2006-2011 (6,046 cycles) and 2012 cohort (1,295 cycles) were included. Clinical (CPR), ongoing pregnancy (OPR) and delivery rates (DR) were stratified by women's age (<35, 35-37, 38-39, 40-41, 42-45 years) and time to blastocyst expansion (<120, 120-129, 130-139, 140-149, >149 hours) as embryo developmental speed. In all the age groups, CPR, OPR, and DR decreased as the embryo developmental speed decreased (P < 0.0001). A simple 5-grade score based on women's age and embryo developmental speed was determined by actual clinical pregnancy rates observed in the 2006-2011 cohort. Subsequently the novel grading score was validated in the 2012 cohort (1295 cycles) finding an excellent association. In conclusion, in the present study we established a novel blastocyst grading system using women's age and embryo developmental speed as objective parameters.

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“…Both animal and human studies have demonstrated that timely entry into the first cleavage (so called Bearly cleavage^) is associated with developmentally more competent embryos compared with late cleavage or no cleavage [37]. Indeed, embryos that cleave between 24 and 27 h after fertilization are more developmentally competent, resulting in higher blastocyst and implantation rates compared with those that cleave relatively later [7,13,[38][39][40][41][42][43][44].…”

Section: Discussionmentioning

confidence: 99%

Is the presence of a non-cleaved embryo on day 3 associated with poorer quality of the remaining embryos in the cohort?

Machtinger

Bormann

Ginsburg

et al. 2015

J Assist Reprod Genet

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Purpose Morphological evaluation is currently considered the single most important predictive measure for assessing embryo quality. The aim of this study was to investigate whether cycles with at least one non-cleaved embryo (i.e., a 1-cell embryo on day 3) have different outcomes compared with cycles in which all embryos had cleaved by day 3. Methods All autologous IVF/ICSI cycles with a fresh day 3 transfer and without using a gestational carrier performed at our center between 1/1/2010 and 12/31/2011 were analyzed retrospectively. Those cycles with at least one non-cleaved embryo on day 3 were compared with all other autologous cycles that had l00 % cleaved embryos performed during the study period. Results Eight hundred and forty two cycles were included. Of them, 144 cycles comprised the non-cleaved group, and 698 cycles comprised the cleaved group. Cycles in the non-cleaved group had more oocytes retrieved (15.4±7.1 vs. 12.5±7.1, p<0.001), more zygotes obtained (10.0±5.3 vs. 7.9±5.2, p= <0.001), but the embryos exhibited lower cleavage rates and higher rates of fragmentation and asymmetry compared with controls (p<0.001). However, spontaneous abortion rates, ectopic pregnancies rates as well as delivery rates were similar between the two groups.Conclusions Our results show that the presence of a noncleaved embryo on day 3 is associated with a more exuberant response to controlled ovarian stimulation as reflected by more oocytes retrieved. Despite the significant decrease in quality of the whole cohort in the non-cleaved group, implantation, delivery rates and number of embryos frozen were not adversely affected by the presence of a non-cleaved embryo.

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Cleavage speed and implantation potential of early-cleavage embryos in IVF or ICSI cycles

Cited by 43 publications

References 18 publications

Improvement of mouse embryo quality by myo-inositol supplementation of IVF media

Improvement of mouse embryo quality by myo-inositol supplementation of IVF media

Women's age and embryo developmental speed accurately predict clinical pregnancy after single vitrified-warmed blastocyst transfer

Is the presence of a non-cleaved embryo on day 3 associated with poorer quality of the remaining embryos in the cohort?

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