Cleavage anomalies in early human embryos and survival after prolonged culture in-vitro

Alikani, Mina; Calderón, Gloria; Tomkin, Giles; Garrisi, John; Kokot, M; Cohen, Jacques

doi:10.1093/humrep/15.12.2634

Cited by 267 publications

(156 citation statements)

References 52 publications

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“…This is usually achieved either by the extended culture of human embryos to the blastocyst stage [5,6] or by the morphological analysis and selection of the highest quality embryos for transfer [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22]. More recently, the assessment of zygote quality has been applied [23][24][25][26][27][28][29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

An oocyte score for use in assisted reproduction

Wilding¹,

Matteo

D’Andretti³

et al. 2007

J Assist Reprod Genet

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Purpose In this work, we describe a system for the morphological scoring of human oocytes prior to fertilisation and use this system to test whether oocyte morphology is an indicator of fertilisation, embryo development and implantation potential. Methods The study is a prospective trial of the use of oocyte morphological scores in 822 patients undergoing their first cycle of ICSI. Analyses of oocytes were performed prior to ICSI procedures and the scores compared with fertilisation rates, embryo quality and clinical results.Results 'Top quality' oocytes had a significantly higher level of fertilisation (96%) as compared to low scoring oocytes (25.6%). Where top quality oocytes formed top quality embryos, we noted a clinical success rate of 63.4%. Conclusions Clinical success rates were increased in cases where top quality oocytes formed top quality embryos after ICSI. The analysis of oocyte morphology may represent a positive selection feature during ICSI.

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Section: Introductionmentioning

confidence: 99%

An oocyte score for use in assisted reproduction

Wilding¹,

Matteo

D’Andretti³

et al. 2007

J Assist Reprod Genet

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“…Multinucleation has also been associated with higher blastomere diameter [1,6], decrease in blastocyst formation rate [5,7,15,19], reduced capacity of development on siblings of MN embryos [2,5], high amount of oocytes retrieved and elevated E 2 levels [2,3,20], follicles hypoxia [21], culture media [13,22], the use of GnRH antagonist [23] and apoptosis [6].…”

Section: Introductionmentioning

confidence: 99%

Insights on blastomere nuclearity

Gil

Gustavo

Póo

et al. 2006

J Assist Reprod Genet

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Purpose: To analyze the results of our transferred embryos, especially those that "changed" their blastomere nuclearity from Multinucleated (MN) to Mono-nucleated during development.Methods: Pregnancies where at least one MN embryo was transferred were retrospectively evaluated and categorized in order to record and follow-up on the ones that were implanted. Embryos were classified as normal (when all blastomeres were mono-nucleated on day one and two of development), corrected (multinucleated embryos on day one that became mono-nucleated on day two) and non-corrected (multinucleated either on day one, on day two or both days).Results: There were 633 transfer cycles analyzed. Thirtythree percent (206) had at least one embryo with a MN blastomere at a given stage of development. Pregnancy and implantation rates were 29.0% and 19.0% for the group of exclusively mono-nucleated embryo transfers, and 28.6% and 15.8% for the group with at least one MN embryo transferred. The pregnancy outcome for "corrected" and "noncorrected" embryos could be corroborated unequivocally in only 9 cases, with an outcome of 8 and 4 normal babies, respectively.Conclusions: Because the amount of data analyzed is not satisfactorily large, differences were not significantly different; however, a trend may exist showing that normal at term pregnancies obtained from corrected embryos are more likely to occur than those from non-corrected embryos. Nuclear observation on a daily basis should be one of the strate-Clínica de Fertilidad, Centro Médico Docente La Trinidad, Caracas, Venezuela e-mail: mgilco@cantv.net gies used to select the best embryos for transferring, to improve implantation rates and avoid multiple pregnancies.

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“…Furthermore, the key adhesion protein, E-cadherin, is first expressed during compaction, enabling even tighter cell-to-cell attachment [47][48][49][50][51]. The obvious morphological changes are accompanied by functional changes due to a shift in gene transcription profile from the maternal to the zygotic genome [52,53]. The transcriptional changes induce cell differentiation processes that result in the inner cell mass and trophectoderm, both essential for blastocyst formation.…”

Section: Initiation Of Early Compactionmentioning

confidence: 99%

Observation of human embryonic behavior in vitro by high‐resolution time‐lapse cinematography

Iwata

Mio²

2016

Reprod Medicine & Biology

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Assisted reproductive technology (ART) has yielded vast amounts of information and knowledge on human embryonic development in vitro; however, still images provide limited data on dynamic changes in the developing embryos. Using our high-resolution time-lapse cinematography (hR-TLC) system, we were able to describe normal human embryonic development continuously from the fertilization process to the hatched blastocyst stage in detail. Our hR-TLC observation also showed the embryonic abnormality of a third polar body (PB)-like substance likely containing a small pronucleus being extruded and resulting in single-pronucleus (1PN) formation, while our molecular biological investigations suggested the possibility that some 1PN embryos could be diploid, carrying both maternal and paternal genomes. Furthermore, in some embryos the extruded third PB-like substance was eventually re-absorbed into the ooplasm resulting in the formation of an uneven-sized, two-PN zygote. In addition, other hR-TLC observations showed that cytokinetic failure was correlated with equal-sized, multi-nucleated blastomeres that were also observed in the embryo showing early initiation of compaction. Assessment combining our hR-TLC with molecular biological techniques enables a better understanding of embryonic development and potential improvements in ART outcomes.

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Cleavage anomalies in early human embryos and survival after prolonged culture in-vitro

Cited by 267 publications

References 52 publications

An oocyte score for use in assisted reproduction

An oocyte score for use in assisted reproduction

Insights on blastomere nuclearity

Observation of human embryonic behavior in vitro by high‐resolution time‐lapse cinematography

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