Cleavage and Gastrulation in the Mouse Embryo

Abstract

After fertilisation of the oocyte, the mouse zygote divides three times before undergoing compaction, which allows polarisation of the blastomeres, and the first lineage specification between trophectoderm (destined to become placenta) and inner cell mass (ICM). The second lineage specification occurs at the blastocyst stage, when ICM cells become either epiblast (which will give rise to the embryo proper) or primitive endoderm (an extraembryonic lineage). After implantation, the proximal (site of attachment with the uterus)‐distal axis is established, quickly followed by the determination of the anterior–posterior axis. Formation of the three main germ layers, ectoderm, mesoderm and endoderm, then occurs on the posterior side at the primitive streak, site of gastrulation. However, recent advances in mouse embryo live imaging have shown that lineage specification was more flexible than previously thought. It has also become possible to visualise the early stages of human embryo development. Key Concepts Early mouse embryo development proceeds through a sequence of lineages specification events. Similar signalling pathways are reused at different steps of early development. Positional and mechanical cues play essential roles for cell lineage specification and morphogenesis. The initial steps, up to and including gastrulation, are quite conserved between all amniotes. The main differences at later stages reside in the extraembryonic tissues, as mammals have adapted to internal gestation through the development of a placenta.

Keratin filaments mediate the expansion of extra‐embryonic membranes in the post‐gastrulation mouse embryo

“…The fetal portion of the placenta comprises trophoblastic, mesenchymal, and vascular cells. Trophoblasts originate from trophectoderm, the outer layer of the blastocyst, that solely contributes to ExE structures including ectoplacental cone and ExE ectoderm (Arnold & Robertson, 2009; Nahaboo & Migeotte, 2018). Mesenchymal and vascular cells originate from the epiblast, a derivative of the blastocyst inner cell mass that is the precursor for most embryonic tissues.…”

Keratin dynamics govern the establishment of the maternal-fetal interface

“…The fetal portion of the placenta comprises trophoblast, mesenchymal and vascular cells, derived from distinct germ layers. Trophoblast (from the Greek words for "feed" and "embryo") originates from trophectoderm, the external layer of the blastocyst stage embryo, that solely contributes to extra-embryonic structures including ectoplacental cone and ExE ectoderm 6,7 . Mesenchymal and vascular cells originate from the epiblast, a germ layer derived from the blastocyst inner cell mass.…”

Exploring and illustrating the mouse embryo: virtual objects to think and create with

“…e.g. Arnold & Robertson (2009); Hashimoto & Nakatsuji (1989); Nahaboo & Migeotte (2018); Saykali et al (2019); Viotti et al (2014)). The embryo as a whole undergoes dramatic shape changes (McDole et al, 2018; Rivera-Pérez & Hadjantonakis, 2014; Snow, 1977).…”