Cleavable ester-linked magnetic nanoparticles for labeling of solvent-exposed primary amine groups of peptides/proteins

Patil, Ujwal S.; Osorno, Laura; Ellender, Angela; Grimm, Casey C.; Tarr, Matthew A.

doi:10.1016/j.ab.2015.05.006

Cited by 7 publications

(2 citation statements)

References 13 publications

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“…Superparamagnetic IONPs have also been used in various biosensing applications to target a broad range of bacteria, viruses, nucleotides, proteins and cancer cells [ 12 ]. Moreover, IONPs have been widely utilized in enrichment and sample preparation steps in proteomics [ 13 , 14 ] and genomics [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro/In Vivo Toxicity Evaluation and Quantification of Iron Oxide Nanoparticles

Patil

Adireddy

Jaiswal

et al. 2015

IJMS

171

117

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Increasing biomedical applications of iron oxide nanoparticles (IONPs) in academic and commercial settings have alarmed the scientific community about the safety and assessment of toxicity profiles of IONPs. The great amount of diversity found in the cytotoxic measurements of IONPs points toward the necessity of careful characterization and quantification of IONPs. The present document discusses the major developments related to in vitro and in vivo toxicity assessment of IONPs and its relationship with the physicochemical parameters of IONPs. Major discussion is included on the current spectrophotometric and imaging based techniques used for quantifying, and studying the clearance and biodistribution of IONPs. Several invasive and non-invasive quantification techniques along with the pitfalls are discussed in detail. Finally, critical guidelines are provided to optimize the design of IONPs to minimize the toxicity.

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Section: Introductionmentioning

confidence: 99%

In Vitro/In Vivo Toxicity Evaluation and Quantification of Iron Oxide Nanoparticles

Patil

Adireddy

Jaiswal

et al. 2015

IJMS

171

117

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“…It is applied in different fields such as chemistry, energy, textile, cosmetics, space research, information technology, biomedicine and agricultural. Recently various types of nanomaterials are widely used in biomedicine for drug discovery (Estelrich et al, 2015) and delivery (Ambwani et al, 2018), bio-imaging (Lobatto et al, 2012), tissue engineering and regenerative medicine (Jayakumar et al, 2011), proteomics (Patil et al, 2015), genomics (Basu et al, 2013), in vitro disease diagnosis (Mamaeva et al, 2018) along with treatment (Kamaly et al, 2016) as well as in stem cell research (Deb et al, 2012). In stem cell research, nanotechnology is applied in stem cell culture (Goto et al, 2007), expansion, differentiation and transplantation (Guo et al, 2017), nano delivery of DNA, RNAi and proteins in stem cells differentiation (Park et al, 2011) as well as in vivo delivery (Nguyen, 2013) and imaging of stem cells (Michalet et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

In vitro CYTOTOXIC EFFECTS OF HYBRID GRAPHENE OXIDE NANOCOMPOSITES (hGONCs) ON CRYOPRESERVED CAPRINE WHARTON’S JELLY DERIVED MESENCHYMAL STEM CELLS (WJ-MSCs)

Dhenge¹,

Gade²,

Op³

et al. 2019

JEBAS

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In present study, caprine Wharton's jelly derived mesenchymal stem cells (WJ-MSCs) were isolated and characterized by osteogenic and adipogenic differentiation. Total 12 hybrid graphene oxide nanocomposites (hGO NCs) such as N2 doped GO-HA NCs, P doped GO-HA NCs, S doped GO-HA NCs, N2 doped GO-SiO2 NCs, P doped GO-SiO2 NCs, S doped GO-SiO2 NCs, N2 doped GO-TiO2 NCs, P doped GO-TiO2 NCs, S doped GO-TiO2 NCs, N2 doped GO-Au NCs, P doped GO-Au NCs and S doped GO-Au NCs were incorporated at doses 100, 50, 25 , 10 and 0 µg/ml in vitrification solution. Caprine WJ-MSCs were cryopreserved by using open pulled straw (OPS) vitrification method and analysed hGO NCs effect on their post thaw viability and culture characteristics. Caprine WJ-MSCs were exhibited normal fibroblastoid morphology and differentiated in to osteogenic and adipogenic lineages. The significant (P<0.01) highest and lowest caprine WJ-MSCs post thaw viability (cumulatively) was observed in P-GO-TiO2 NC and P-GO-HA NC groups, respectively along with all doses (cumulatively) significantly (P<0.01) decreased post thaw cell viability as compared with control. All hGO NCs were significantly (P<0.01) decreased caprine WJ-MSCs post thaw viability at doses 100 and 50 µg/ml as compared to 25, 10 and 0 µg/ml doses. Post thaw caprine WJ-MSCs were grew in normal pattern with similar fibroblast like morphology on days 14. In conclusion, all hGO NCs at doses 50 and 100 µl/ml are cytotoxic and P-GO-TiO2 NC is least decrease caprine WJ-MSCs post thaw viability.

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Biomedical Applications of Nanomaterials

Norman

Albert

Perumal

et al. 2022

Handbook of Green and Sustainable Nanotechnology

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Cleavable ester-linked magnetic nanoparticles for labeling of solvent-exposed primary amine groups of peptides/proteins

Cited by 7 publications

References 13 publications

In Vitro/In Vivo Toxicity Evaluation and Quantification of Iron Oxide Nanoparticles

In Vitro/In Vivo Toxicity Evaluation and Quantification of Iron Oxide Nanoparticles

In vitro CYTOTOXIC EFFECTS OF HYBRID GRAPHENE OXIDE NANOCOMPOSITES (hGONCs) ON CRYOPRESERVED CAPRINE WHARTON’S JELLY DERIVED MESENCHYMAL STEM CELLS (WJ-MSCs)

Biomedical Applications of Nanomaterials

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