Clearance function of scavenger endothelial cells

Smedsrød, B

doi:10.1186/1476-5926-2-s1-s22

Cited by 108 publications

(30 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[38][39][40] In this experiment, DNA-MP-1.0 was treated to cells and incubated with the cells for 8 h. In previous studies, microparticles with a size of 1-2 mm were seen to preferentially internalize into cells not by endocytosis, but by phagocytosis. 38,41 Some DNA-MPs were co-localized with EEA1 probably due to localization in the early phagosomes. Others were shown by red-uorescence in the cells, which might be due to their localization in late phagosomes (bottom panels of Fig.…”

Section: Intracellular Uptake Of Cpg Based Dna-mpsmentioning

confidence: 99%

CpG incorporated DNA microparticles for elevated immune stimulation for antigen presenting cells

et al. 2018

View full text Add to dashboard Cite

show abstract

Section: Intracellular Uptake Of Cpg Based Dna-mpsmentioning

confidence: 99%

CpG incorporated DNA microparticles for elevated immune stimulation for antigen presenting cells

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The nearly neutral charge is precious for in vivo use, as large positive or negative charges may be rapidly cleared from the blood. Positively charged materials cause non-specific cell sticking, while those negatively charged are efficiently taken up by scavenger endothelial cells or ''professional pinocytes'' found in liver [37].…”

Section: Size and Surface Charge In Different Environmentsmentioning

confidence: 99%

Supramolecular assembled nanogel made of mannan

Ferreira

Pereira

Sampaio

et al. 2011

Journal of Colloid and Interface Science

View full text Add to dashboard Cite

The supramolecular assembly of amphiphilic mannan, synthesized by the Michael addition of hydrophobic 1-hexadecanethiol to vinyl methacrylated mannan, originates in aqueous medium the formation of a nanogel, stabilized by hydrophobic interactions among alkyl chains. The critical aggregation concentration, calculated by fluorescence spectroscopy ranged between 0.002 and 0.01 mg/mL, depending on the polymer degree of substitution. The cryo-field emission scanning electron microscopy showed spherical macromolecular micelles with diameters between 100 and 500 nm. The dynamic light scattering analysis revealed a polydisperse colloidal system, with mean hydrodynamic diameter between 50 and 140 nm, depending on the polymer degree of substitution. The nanogel is negatively charged, stable over a 6 months storage period, and stable at pH 3-8, salt or urea solutions. Bovine serum albumin and curcumin were spontaneously incorporated in the nanogel, being stabilized by the hydrophobic domains, opening the possibility for future applications as potential delivery systems for therapeutic molecules. In vitro assays were carried out to characterize the biocompatibility of the nanogel. A toxic effect of mannan-C(16) was observed, specific to mouse macrophage-like cell line J774, not affecting mouse embryo fibroblast cell line 3T3 viability.

show abstract

“…Being highly specialized in metabolic function to generate proteins, lipids and carbohydrates together with elimination of toxic compounds via bile, hepatocytes require complementary help by other liver cell populations to perform non-metabolic functions. Liver sinusoidal endothelial cells (LSECs) are the most efficient endocytic cell population of the body; they scavenge molecules from the bloodstream 5 , 6 , 7 and then transport them in a trans-cytotic manner from the sinusoidal lumen to the hepatocyte surface. 8 By doing so, LSECs assist the metabolic function of hepatocytes by allowing much more efficient extraction of molecules from the blood stream than what is limited by simple diffusion across endothelial fenestrae, which allows for direct access of blood-borne molecules to the space of Dissé and hepatocytes.…”

Section: Hepatic Microanatomy Is Linked To Physiological Liver Functimentioning

confidence: 99%

“…LSECs have the highest endocytic uptake in the body, 6 , 17 and also outpace professional antigen-processing immune cell populations such as dendritic cells or myeloid cells, in terms of uptake of circulating antigens. 18 Being equipped with different classes of scavenger receptors, as well as the mannose receptor, LSECs can extract a broad variety of molecules from blood percolating through the liver.…”

Section: Scavenger and Sentinel Function Of Lsecsmentioning

confidence: 99%

Immunological functions of liver sinusoidal endothelial cells

2016

View full text Add to dashboard Cite

Liver sinusoidal endothelial cells (LSECs) line the liver sinusoids and separate passenger leukocytes in the sinusoidal lumen from hepatocytes. LSECs further act as a platform for adhesion of various liver-resident immune cell populations such as Kupffer cells, innate lymphoid cells or liver dendritic cells. In addition to having an extraordinary scavenger function, LSECs possess potent immune functions, serving as sentinel cells to detect microbial infection through pattern recognition receptor activation and as antigen (cross)-presenting cells. LSECs cross-prime naive CD8 T cells, causing their rapid differentiation into memory T cells that relocate to secondary lymphoid tissues and provide protection when they re-encounter the antigen during microbial infection. Cross-presentation of viral antigens by LSECs derived from infected hepatocytes triggers local activation of effector CD8 T cells and thereby assures hepatic immune surveillance. The immune function of LSECs complements conventional immune-activating mechanisms to accommodate optimal immune surveillance against infectious microorganisms while preserving the integrity of the liver as a metabolic organ.

show abstract

Clearance function of scavenger endothelial cells

Cited by 108 publications

References 66 publications

CpG incorporated DNA microparticles for elevated immune stimulation for antigen presenting cells

CpG incorporated DNA microparticles for elevated immune stimulation for antigen presenting cells

Supramolecular assembled nanogel made of mannan

Immunological functions of liver sinusoidal endothelial cells

Contact Info

Product

Resources

About