Clearance and Distribution of Acid-Stable Trypsin Inhibitor
(ASTI)

Sugiki, Masahiko; Sumi, Hiroyuki; Maruyama, Masugi; Yoshida, Etsuo; Mihara, Hisashi

doi:10.1159/000469004

Cited by 29 publications

(19 citation statements)

References 6 publications

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“…plasma is linked with the heavy chains (ITI and P␣I) (8), and the released bikunin in circulation is rapidly excreted into urine (9), supports this notion. The influx of plasma into preovulatory follicles and the local formation of the SHAP-HA complex recalls similar situations in inflammatory sites, where cytokines stimulate local HA synthesis and induce capillary hyperpermeability to allow the efflux of plasma components.…”

Section: Physiological Functions Of the Shap-ha Complex And Iti Familmentioning

confidence: 78%

“…4C). In contrast, administration of human UTI resulted in no improvement of the naked oocytes (5 mice), although the rapid clearance of UTI from blood should not be overlooked (9). The recovery could be explained by the formation of the SHAP-HA complex between the exogenous ITI and the endogenous cumulus HA in accordance with the test tube assay (Fig.…”

Section: Iti Administration Fully Rescued the Defect Of Cumulus Expanmentioning

confidence: 91%

“…The members are composed of a common light chain, bikunin (Bik), and one or two of the three genetically different heavy chains (HC1, HC2, and HC3) (8). Free bikunin in circulation is excreted rapidly into the urine (9) where it is present as the urinary trypsin inhibitor (UTI) (10). The serine residue at position 10 of bikunin contains an Oglycosidically linked chondroitin-4-sulfate (CS) chain (11), to which the C terminal aspartate of a heavy chain is covalently bound via a unique ester bond (12).…”

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confidence: 99%

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Defect in SHAP-Hyaluronan Complex Causes Severe Female Infertility

Zhuo

Yoneda

Zhao

et al. 2001

Journal of Biological Chemistry

256

251

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Hyaluronan (HA) associates with proteins and proteoglycans to form the extracellular HA-rich matrices that significantly affect cellular behaviors. So far, only the heavy chains of the plasma inter-␣-trypsin inhibitor (ITI) family, designated as SHAPs (serum-derived hyaluronan-associated proteins), have been shown to bind covalently to HA. The physiological significance of such a unique covalent complex has been unknown but is of great interest, because HA and the ITI family are abundant in tissues and in plasma, respectively, and the SHAP-HA complex is formed wherever HA meets plasma. We abolished the formation of the SHAP-HA complex in mice by targeting the gene of bikunin, the light chain of the ITI family members, which is essential for their biosynthesis. As a consequence, the cumulus oophorus, an investing structure unique to the oocyte of higher mammals, had a defect in forming the extracellular HA-rich matrix during expansion. The ovulated oocytes were completely devoid of matrix and were unfertilized, leading to severe female infertility. Intraperitoneal administration of ITI, accompanied by the formation of the SHAP-HA complex, fully rescued the defects. We conclude that the SHAP-HA complex is a major component of the HA-rich matrix of the cumulus oophorus and is essential for fertilization in vivo.

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Section: Physiological Functions Of the Shap-ha Complex And Iti Familmentioning

confidence: 78%

Section: Iti Administration Fully Rescued the Defect Of Cumulus Expanmentioning

confidence: 91%

mentioning

confidence: 99%

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Defect in SHAP-Hyaluronan Complex Causes Severe Female Infertility

Zhuo

Yoneda

Zhao

et al. 2001

Journal of Biological Chemistry

256

251

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“…9,10 It is also one of the acute phase reactants. 11,12 The amount of this inhibitor excreted in the urine is considered proportional to the invasiveness of an insult to the host, typically surgery.…”

Section: Discussionmentioning

confidence: 99%

Urinary trypsin inhibitor concentration can predict the immunological insult of chemotherapy and complications after bone marrow transplantation

Yamada

Takatsuka

Takemoto

et al. 2001

Bone Marrow Transplant

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Summary:Urinary trypsin inhibitor has attracted attention as an index of the systemic inflammatory response syndrome. In this study, the urine concentration of trypsin inhibitor was measured to compare the immunological insult of conventional chemotherapy and conditioning chemotherapy for bone marrow transplantation. We also investigated whether urinary trypsin inhibitor was a useful index of the complications and outcome of bone marrow transplantation. Urinary trypsin inhibitor concentration was determined before chemotherapy, on the day after finishing chemotherapy (day 0 of transplantation), and during recovery of the white cell count, in 17 patients ( seven receiving conventional chemotherapy and 10 receiving conditioning for bone marrow transplantation). Urinary trypsin inhibitor concentrations were significantly higher after conditioning for bone marrow transplantation than after conventional chemotherapy (P Ͻ 0.001), indicating that conditioning was more invasive. After bone marrow transplantation, the incidence of severe complications and the mortality rate were higher in patients whose urinary trypsin inhibitor concentrations rose during recovery of the white cell count. Comparison of urinary trypsin inhibitor concentrations suggested that conditioning for bone marrow transplantation was more invasive than conventional chemotherapy. This study also suggested that the urine concentration of trypsin inhibitor could be useful for predicting the risk of complications and outcome of bone marrow transplantation. Bone Marrow Transplantation (2001) 27, 195-199. Keywords: allogeneic bone marrow transplantation; systemic inflammatory response syndrome; urinary trypsin inhibitor; interleukin-6; C-reactive protein Bone marrow transplantation (BMT) is not only an established therapy for hematological malignancies, but also for Recently, we investigated the relationship between cytokines and vascular endothelial cell dysfunction after BMT and advocated the concept that complications of BMT are collectively caused by the systemic inflammatory response syndrome (SIRS).1-5 The urine concentration of trypsin inhibitor (TI) is known to be very closely associated with the extent of surgical invasion and also reflects the severity of SIRS. 6 In the present study, the usefulness of urinary TI as a predictor of complications and the outcome of BMT was assessed by measuring the concentrations of this marker before and after transplantation. Materials and methods SubjectsThe subjects were 10 consecutive patients who underwent allogeneic BMT from March 1999 to December 1999 and seven consecutive patients who were treated by conventional chemotherapy from September 1999 to December 1999, at our institution. Tables 1 and 2 show the age, gender, underlying disease, and disease stage. These also show the donor, conditioning regimen, and GVHD prophylaxis for BMT patients, as well as the chemotherapy regimen for those given conventional chemotherapy. The basic conditioning regimen was busulfan (BU), cyclophosphamide (CY), and total bo...

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“…However, more than 90% of circulating bikunin carries HCs, whereas urinary bikunin is predominantly free of HCs (46). Furthermore, free bikunin has a much shorter half-life in circulation (4 min in mice) than that of HC-bikunin complexes (several hours in mice) (47,48). This paradox is explained by recent studies of I␣I-HA interactions.…”

Section: Protease Inhibitor Activity Of I␣i Family Moleculesmentioning

confidence: 99%