In conventional superconductors, the superconducting gap in the electronic excitation spectrum prevents scattering of low-energy electrons. In high-temperature superconductors (HTSs), an extra gap, the pseudogap 1 , develops well above the superconducting transition temperature T C . Here, we present a new avenue of investigating the pseudogap state, using scanning tunnelling microscopy (STM) of resonances generated by single-atom scatterers. Previous studies on the superconducting state of HTSs 2 have led to a fairly consistent picture in which potential scatterers, such as Zn, strongly suppress superconductivity in an atomic-scale region, while generating low-energy excitations with a spatial distribution-as imaged by STM 3,4 -indicative of the d-wave nature of the superconducting gap. Surprisingly, we find that similar native impurity resonances coexist spatially with the superconducting gap at low temperatures and survive virtually unchanged on warming through T C . These findings demonstrate that properties of impurity resonances in HTSs are not determined by the nature of the superconducting state, as previously suggested, but instead provide new insights into the pseudogap state.In d-wave superconductors, such as the high-temperature superconductors (HTSs), impurities act as pair breakers, giving rise to virtual bound states, or resonances, within the gap. For strong scatterers, these resonances lie close to the Fermi energy, and significantly modify bulk superconducting properties 5,6 . The local (atomic scale) effects of these resonances have been studied by several probes, such as nuclear magnetic resonance 7-11 (NMR) and muon spin relaxation (µSR) 12 . A variety of scanning tunnelling microscopy (STM) studies of impurity resonances in HTSs have been reported, including studies of native (unidentified) impurities 13,14 , intentionally doped Zn and Ni impurities 3,4 and intentionally placed surface impurities 15 . All of these STM studies demonstrated that impurity resonances are associated with an enhanced local density of states inside the gap, close to the Fermi energy. All of these studies were also carried out on Bi 2 Sr 2 CaCu 2 O 8+x (Bi-2212) near 4 K, significantly below T C .Here, we report on temperature-dependent STM studies of native impurities in overdoped (T C = 15 K) Bi 2−y Pb y Sr 2 CuO 6+x (Bi-2201). In addition to enabling comparison to previous studies in Bi-2212, Bi-2201 has the benefit of having a relatively low T C , thus enabling us to study impurity resonances below and above T C without the resonance being obscured by thermal broadening.To carry out the temperature-dependent measurements discussed here we have constructed an ultrahigh-vacuum STM with the ability to track atomically resolved regions-here surrounding individual impurities-over a wide range of temperatures. We begin our study at low temperatures, using an experimental methodology similar to that used in previous STM impurity studies 3,4 . We search for impurity resonances by recording a spectral survey, in which d...