Cleaning of titanium substrates after application in a bioreactor

Fingerle, Mathias; Köhler, Oliver; Rösch, Christina; Kratz, Fabian; Scheibe, Christian; Davoudi, Neda; Müller‐Renno, Christine; Ziegler, Christiane; Huster, Manuel; Schlegel, Christin; Ulber, Roland; Bohley, Martin; Aurich, Jan C.

doi:10.1116/1.4907754

Cited by 8 publications

(12 citation statements)

References 18 publications

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“…11 The peak at around 400.5 eV is typical for amide and amine and can serve as a marker. 4 The core level spectra clearly show that the detected nitrogen is not bound in the form of nitrate (from HNO 3 ) (407.3 eV). 12 However, this nitrogen peak at 400.5 eV can also be found on the standard cleaned, plasma cleaned, and base acid cleaned FIG.…”

Section: Biofilm and Bacteria Removalmentioning

confidence: 99%

“…4 XPS and contact angle measurements demonstrate that oxygen plasma generates a strongly oxidized surface, which changes the surface properties and thus the surface chemistry in an uncontrollable way due to the complex alloy composition. A constructive way of cleaning is a combination of base and acid at elevated temperatures that does not change the surface chemistry.…”

Section: C404-8 Huttenlochner Et Almentioning

confidence: 99%

“…Here, the N1s photoelectron line of nitrogen bound in an amine at 400.5 eV was used as a marker for the presence of a biofilm. 4 For these nitrogen core level spectra, measured on unpolished steel samples using different cleaning procedures, a polished steel sample with a 7 day grown biofilm and the cleaned biofilm sample are compared in Fig. 6.…”

Section: Biofilm and Bacteria Removalmentioning

confidence: 99%

“…In a previous paper, we have already shown that cleaning of unstructured and microstructured titanium substrates with oxygen plasma presents an effective cleaning procedure without changing the surface properties. 4 Bioreactors are usually made of stainless steel, e.g., of the stainless austenitic steel 1.4571. Thus, plasma cleaning was also the first choice to remove biofilms of the sea water bacterium Paracoccus seriniphilus from such stainless steel surfaces.…”

Section: Introductionmentioning

confidence: 99%

“…Additional contributions can be described by two overlapping Gaussian peaks at 288.1 eV (O-C-O and C¼O bonds) and 289.2 eV (carboxylic or ester bonds and contributions of adsorbed water). 4 …”

mentioning

confidence: 99%

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Removing biofilms from stainless steel without changing surface properties relevant for bacterial attachment

et al. 2017

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The influence of oxygen (and argon) plasma cleaning and a base-acid cleaning procedure on stainless steel surfaces was studied. The main aim was to clean stainless steel samples from Paracoccus seriniphilus biofilms without changing the surface properties which are relevant for bacterial attachment to allow reuse in a biofilm reactor. It is shown that oxygen plasma cleaning, which very successfully removes the same kind of biofilm from titanium surfaces, is not suitable for stainless steel. It largely influences the surface chemistry by producing thick metal oxide layers of varying compositions and changing phenomenological surface properties such as wettability. A promising method without changing surface properties while cleaning satisfactorily is a combination of base and acid reagents at elevated temperature.

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Section: Biofilm and Bacteria Removalmentioning

confidence: 99%

Section: C404-8 Huttenlochner Et Almentioning

confidence: 99%

Section: Biofilm and Bacteria Removalmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Removing biofilms from stainless steel without changing surface properties relevant for bacterial attachment

et al. 2017

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Analyzing the influence of microstructured surfaces on the lactic acid production of Lactobacillus delbrueckii lactis in a flow‐through cell system

Schlegel

Chodorski

Huster

et al. 2017

Engineering in Life Sciences

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Microorganisms growing in biofilms might be possible biocatalysts for future biotechnological production processes. Attached to a surface and embedded in an extracellular polymeric matrix, they create their preferred environment and form robust cultures for continuous systems. With the objective of implementing highly efficient processes, productive biofilms need to be understood comprehensively. In this study, the influence of microstructured metallic surfaces on biofilm productivity was researched. To conduct this study, titanium and stainless steel sheets were polished, micromilled, as well as coated with particles. Subsequently, the metal sheets were exposed to the lactic acid producing Lactobacillus delbrueckii subsp. lactis under laminar and homogeneous flow conditions in a custom-built flow cell. A proof-ofconcept showed that biofilm formation in the system only occurred on the designated substratum. Following a 24-h batch cultivation for primary biofilm development, the culture was continuously provided with glucose containing medium. As different experimental series have indicated, the process resulted to be stable for up to eleven days. Primary metabolite productivity averaged around 6-7 g/(L h). Interestingly, the productivity was shown to be affected neither by the type of metal, nor by the applied microstructures. Nevertheless, a higher dry biomass weight determined on micro-milled substratum indicates a complementary differentiation of biofilm components in future experiments.

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Monitoring of biofilms grown on differentially structured metallic surfaces using confocal laser scanning microscopy

Kleine

Chodorski

Mitra

et al. 2019

Engineering in Life Sciences

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Imaging of biofilms on opaque surfaces is a challenge presented to researchers especially considering pathogenic bacteria, as those typically grow on living tissue, such as mucosa and bone. However, they can also grow on surfaces used in industrial applications such as food production, acting as a hindrance to the process. Thus, it is important to understand bacteria better in the environment they actually have relevance in. Stainless steel and titanium substrata were line structured and dotted surface topographies for titanium substrata were prepared to analyze their effects on biofilm formation of a constitutively green fluorescent protein (GFP)‐expressing Escherichia coli strain. The strain was batch cultivated in a custom built flow cell initially for 18 h, followed by continuous cultivation for 6 h. Confocal laser scanning microscopy (CLSM) was used to determine the biofilm topography. Biofilm growth of E. coli GFPmut2 was not affected by the type of metal substrate used; rather, attachment and growth were influenced by variable shapes of the microstructured titanium surfaces. In this work, biofilm cultivation in flow cells was coupled with the most widely used biofilm analytical technique (CLSM) to study the time course of growth of a GFP‐expressing biofilm on metallic surfaces without intermittent sampling or disturbing the natural development of the biofilm.

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Cleaning of titanium substrates after application in a bioreactor

Cited by 8 publications

References 18 publications

Removing biofilms from stainless steel without changing surface properties relevant for bacterial attachment

Removing biofilms from stainless steel without changing surface properties relevant for bacterial attachment

Analyzing the influence of microstructured surfaces on the lactic acid production of Lactobacillus delbrueckii lactis in a flow‐through cell system

Monitoring of biofilms grown on differentially structured metallic surfaces using confocal laser scanning microscopy

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