Clean-in-place disinfection of dual-species biofilm (Listeria and Pseudomonas) by a green antibacterial product made from citrus extract

Medina-Rodríguez, Andrea C.; Ávila-Sierra, Alejandro; Ariza, Juan José; Guillamón, Enrique; Baños, Alberto; Vicaria, José M.; Jurado, E.

doi:10.1016/j.foodcont.2020.107422

Cited by 19 publications

(7 citation statements)

References 35 publications

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“…More than 90% of bacteria grow to form biofilms naturally, 1 which is a microbial community that irreversibly adheres to the surface of material or tissue by self-secreted extracellular polymeric substances (EPS). 2 EPS include exopolysaccharides, proteins, and extracellular DNA (eDNA), 3–5 which serves as a natural barrier against the penetration of antibiotics and cellular attack by host innate immune cells. 6 , 7 Biofilm formation involves a series of complex processes, including surface attachment of planktonic bacteria, proliferation of adherent cells and production of EPS, formation of small colonies and mature biofilm, and diffusion of mature biofilms.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Antibacterial and Anti-Biofilm Activities of Antimicrobial Peptides Modified Silver Nanoparticles

Xu¹,

Li²,

Wang³

et al. 2021

IJN

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Background:The biofilms could protect bacteria from antibiotics and promote the production of drug-resistant strains, making the bacteria more difficult to be eradicated. Thus, we developed an AMP@PDA@AgNPs nanocomposite, which is formed by modifying silver nanoparticles (AgNPs) with antimicrobial peptides (AMP) modified nanocomposite to destroy biofilm in this study. Methods: The AMP@PDA@AgNPs nanocomposite was prepared with polymerization method and characterized by using ultraviolet-visible (UV-vis) spectroscopy, dynamic light scattering (DLS), Fourier transform-infrared spectroscopy (FT-IR), and transmission electron microscope (TEM). The antibacterial effects of the nanocomposite were investigated by using agar diffusion method and minimum inhibitory concentration (MIC) test. The quantitative analysis of the biofilm formation by the nanocomposite was conducted using crystal violet staining and confocal laser scanning microscope (CLSM). Results: The DLS and TEM analysis showed it was a spherical nanocomposite with 200 nm size and well dispersed . The results of UV-vis and FT-IR confirmed the presence of AMP and AgNPs. The nanocomposite had an excellent biocompatibility at 100 μg/mL. And the AMP@PDA@AgNPs nanocomposite showed superior antimicrobial activity against both Gram-negative (E. coli, P. aeruginosa) and Gram-positive (S. aureus) bacteria than AgNPs or AMP. Importantly, the mRNA expression of biofilm-related genes were decreased under the action of the nanocomposites. Conclusion: An AMP@PDA@AgNPs nanocomposite with good biocompatibility was successfully prepared. The nanocomposite could destruct bacterial biofilms by inhibiting the expression of biofilm-related genes. The synergistic strategy of AMPs and AgNPs could provide a new perspective for the treatment of bacterial infection.

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Section: Introductionmentioning

confidence: 99%

Enhanced Antibacterial and Anti-Biofilm Activities of Antimicrobial Peptides Modified Silver Nanoparticles

Xu¹,

Li²,

Wang³

et al. 2021

IJN

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“…The COD value was analysed according to Avila-Sierra et al(2020). 4 Solution samples of 1.5 mL were extracted periodically, and added to 1.5 mL of distilled water and mixed with the reagent solution. The reagent solution was formed by mixing 0.08 g Hg 2 SO 4 (Panreac), 4 mL of sulfuric acid reagent (10 g L -1 Ag 2 SO 4 dissolved in H 2 SO 4 ) and 1 mL of 0.5 N K 2 Cr 2 O 7 aqueous solution.…”

Section: Evaluation Of Chemical Oxygen Demand (Cod)mentioning

confidence: 99%

Kinetic modeling of advanced starch oxidation with ozone in basic solutions

Ávila-Sierra

Vicaria

Martínez-Gallegos

et al. 2022

Preprint

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Ozonation of cornstarch at pH 13 was performed using a semi-batch reactor to analyse the influence of a continuous ozone-oxygen gas flow (40 NL/h) at different ozone concentrations (0-42.30 g/Nm3) and temperatures (20-60ºC). A pseudofirst-order kinetic model was proposed to elucidate the reaction mechanisms associated with ozone at high pH conditions. The radical concentration remains constant and proportional to the ozone continuous dose, being a non-limiting factor independent of temperature (activation energy 14.2-14.5 KJ/mol). The oxidation kinetic of starch was studied monitoring the Chemical Oxygen Demand, finding a reduction in ozone concentration when both temperature and time increased (activation energy 8.1 KJ/mol). Therefore, the model proposed for the starch ozonation at high alkaline solutions provides a better understanding of the oxidation processes of common product additives such as starch, also making it possible to determine the environmental impact of such solutions by Chemical Oxygen Demand measurement.

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“…Additionally, the bitter orange extract, in addition to citric acid, contains naringenin as well -a type of flavonoid with antibacterial properties (Medina-Rodriguez, et al, 2020).…”

Section: Effectiveness Of Cleaning Agentsmentioning

confidence: 99%