Clays as Biomaterials in Controlled Drug Release: A Scientific and Technological Short Review

Silva, Fabrícia de Castro; Lima, Luciano Clécio Brandão; Honorio, Luzia M. C.; Trigueiro, Pollyana; Osajima, Josy Anteveli; Lobo, Anderson Oliveira; Filho, Edson Cavalcanti da Silva

doi:10.26717/bjstr.2019.15.002677

Cited by 5 publications

(2 citation statements)

References 57 publications

(87 reference statements)

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“…Sepiolite is a more robust transformation material with more reports in the literature than arginine-glucose functionalized hydroxyapatite nanoparticles and has a 5.9 times higher transformation efficiency than Mg aminoclays using the same host and plasmid (Mendes et al, 2015). However, Mg aminoclays have a higher potential than sepiolite for acquiring enhanced properties that can become a complement to transformation of microorganisms as it has been demonstrated in their use as biosensors (Mann, 2009;Mousty, 2010;Song et al, 2018) or drug delivery nanocarriers (Patil et al, 2005;Patil and Mann, 2008;Chaturbedy et al, 2010;Silva Filho, 2019), making aminoclay-based materials more promising for future implementations of DNA delivery agents since these additional properties can potentially be exploited synergistically with transformation during interaction with cells.…”

Section: Transformation Of E Coli Jm109 With Puc19 Plasmid Using Mg Aminoclaysmentioning

confidence: 99%

Magnesium aminoclays as plasmid delivery agents for non-competent Escherichia coli JM109 transformation

Mendes

Kluskens

Lanceros‐Méndez

et al. 2021

Applied Clay Science

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Magnesium aminoclays were synthesized and used to transform non-competent Escherichia coli JM109 using the exogenous plasmid pUC19. The structure determined for the Mg aminoclays is analogous to 2:1 trioctahedral smectites such as talc, with an approximate composition R 8 Si 8 Mg 6 O 16 (OH) 4 , where R = CH 2 CH 2 NH 2 , morphologically arranged in layered sheets. Mg aminoclays were employed as a cationic vehicle that enabled the passage of plasmids across the cell envelope and led to genetic modification of the host. A stock solution of 10 mg/mL of Mg aminoclays was prepared, mixed with E. coli JM109 and pUC19 plasmid, and spread over Petri dishes containing lysogeny broth (LB), isopropyl β-D-1-thiogalactopyranoside (IPTG), 5-bromo-4-chloro-3indolyl-β-D-galactopyranoside (X-gal), ampicillin and various concentrations of agar (1-4%). The transformation efficiency obtained was higher for 1% and 2% agar even though transformation also occurred at agar concentrations of 3% and 4%. The optical density of E. coli JM109 and spreading time were also adjusted, favoring transformation when cells were used in their exponential growth phase (OD 600 = 1.0) and spread for 90 s. Transformation was confirmed by the growth of blue colonies in LB/IPTG/X-gal/agar Petri dishes containing ampicillin, by regrowth of biomass in liquid media containing ampicillin and by agarose gel electrophoresis of the linearized pUC19 plasmid that followed plasmidic DNA extraction from 4 blue colonies. The maximum transformation efficiency achieved was 7.0 × 10 3 CFU/μg pUC19. This transformation approach proved to be suitable for a convenient, cost-effective, room-temperature, risk-free and rapid transformation of non-competent E. coli JM109.

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Section: Transformation Of E Coli Jm109 With Puc19 Plasmid Using Mg Aminoclaysmentioning

confidence: 99%

Magnesium aminoclays as plasmid delivery agents for non-competent Escherichia coli JM109 transformation

Mendes

Kluskens

Lanceros‐Méndez

et al. 2021

Applied Clay Science

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“…Different materials for controlled release are reported in the literature, which have in common the use of phyllosilicates, which is justified because these materials are abundant in nature, are low cost, non-toxic, and have the ability to adsorb ions and/or molecules in their interlamellar space [10,11]. In addition, there is the potential diversity of their use, which is due to the ease with which these materials are modified [12,13,14,15].…”

Section: Introductionmentioning

confidence: 99%

Hybrid Systems Based on Talc and Chitosan for Controlled Drug Release

Lima

Coêlho

Silva³

et al. 2019

Materials

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Inorganic matrices and biopolymers have been widely used in pharmaceutical fields. They show properties such as biocompatibility, incorporation capacity, and controlled drug release, which can become more attractive if they are combined to form hybrid materials. This work proposes the synthesis of new drug delivery systems (DDS) based on magnesium phyllosilicate (Talc) obtained by the sol–gel route method, the biopolymer chitosan (Ch), and the inorganic-organic hybrid formed between this matrix (Talc + Ch), obtained using glutaraldehyde as a crosslink agent, and to study their incorporation/release capacity of amiloride as a model drug. The systems were characterized by X-ray diffraction (XRD), Therma analysis TG/DTG, and Fourier-transform infrared spectroscopy (FTIR) that supported the DDS’s formation. The hybrid showed a better drug incorporation capacity compared to the precursors, with a loading of 55.74, 49.53, and 4.71 mg g−1 for Talc + Ch, Talc, and Ch, respectively. The release assays were performed on a Hanson Research SR-8 Plus dissolver using apparatus I (basket), set to guarantee the sink conditions. The in vitro release tests showed a prolongation of the release rates of this drug for at least 4 h. This result proposes that the systems implies the slow and gradual release of the active substance, favoring the maintenance of the plasma concentration within a therapeutic window.

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Tunable particle size synthesis of nanoclay from Sidoarjo geothermal mud via ultrasonic method

Silvia

Jenie²,

Petrus

2022

The International Conference on Advanced Material and Technology (Icamt) 2021

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Clays as Biomaterials in Controlled Drug Release: A Scientific and Technological Short Review

Cited by 5 publications

References 57 publications

Magnesium aminoclays as plasmid delivery agents for non-competent Escherichia coli JM109 transformation

Magnesium aminoclays as plasmid delivery agents for non-competent Escherichia coli JM109 transformation

Hybrid Systems Based on Talc and Chitosan for Controlled Drug Release

Tunable particle size synthesis of nanoclay from Sidoarjo geothermal mud via ultrasonic method

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