Clavibacter michiganensis, a group of gram-positive phytopathogenic bacteria

Eichenlaub, Rudolf; Gartemann, Karl-Heinz; Burger, A.

doi:10.1007/978-1-4020-4538-7_12

Cited by 54 publications

(43 citation statements)

References 99 publications

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“…The plant-pathogenic bacterium Clavibacter michiganensis, the only species in the genus Clavibacter, includes five subspecies which are defined with respect to their host plants: subsp. michiganensis (Solanum lycopersicum), nebraskensis (Zea mays), insidiosus (Medicago sativa), tessellarius (Tritium aestivum) and sepedonicus (Solanum tuberosum) (Davis et al, 1984;Lee et al, 1997;Eichenlaub et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

The endolysins of bacteriophages CMP1 and CN77 are specific for the lysis of Clavibacter michiganensis strains

2010

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Putative endolysin genes of bacteriophages CMP1 and CN77, which infect Clavibacter michiganensis subsp. michiganensis and C. michiganensis subsp. nebraskensis, respectively, were cloned and expressed in Escherichia coli. The His-tagged endolysin of CMP1 consists of 306 amino acids and has a calculated molecular mass of 34.8 kDa, while the His-tagged endolysin of CN77 has 290 amino acids with a molecular mass of 31.9 kDa. The proteins were purified and their bacteriolytic activity was demonstrated. The bacteriolytic activity of both enzymes showed a host range which was limited to the respective C. michiganensis subspecies and did not affect other bacteria, even those closely related to Clavibacter. Due to the high specificity of the CMP1 and CN77 endolysins they may be useful tools for biocontrol of plantpathogenic C. michiganensis without affecting other bacteria in the soil.

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Section: Introductionmentioning

confidence: 99%

The endolysins of bacteriophages CMP1 and CN77 are specific for the lysis of Clavibacter michiganensis strains

2010

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“…This genus currently includes only one species divided into five subspecies, all of which are plant pathogens of specific hosts. All subspecies cause systemic infections of the xylem which may be latent without visible symptoms, seem to be able to invade seeds, and show only poor survival capabilities in soil (14,60). They may have an epiphytic lifestyle (25).…”

mentioning

confidence: 99%

The Genome Sequence of the Tomato-Pathogenic ActinomyceteClavibacter michiganensissubsp.michiganensisNCPPB382 Reveals a Large Island Involved in Pathogenicity

Gartemann¹,

Abt²,

Bekel³

et al. 2008

J Bacteriol

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149

171

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Clavibacter michiganensis subsp. michiganensis is a plant-pathogenic actinomycete that causes bacterial wilt and canker of tomato. The nucleotide sequence of the genome of strain NCPPB382 was determined. The chromosome is circular, consists of 3.298 Mb, and has a high G؉C content (72.6%). Annotation revealed 3,080 putative protein-encoding sequences; only 26 pseudogenes were detected. Two rrn operons, 45 tRNAs, and three small stable RNA genes were found. The two circular plasmids, pCM1 (27.4 kbp) and pCM2 (70.0 kbp), which carry pathogenicity genes and thus are essential for virulence, have lower G؉C contents (66.5 and 67.6%, respectively). In contrast to the genome of the closely related organism Clavibacter michiganensis subsp. sepedonicus, the genome of C. michiganensis subsp. michiganensis lacks complete insertion elements and transposons. The 129-kb chp/tomA region with a low G؉C content near the chromosomal origin of replication was shown to be necessary for pathogenicity. This region contains numerous genes encoding proteins involved in uptake and metabolism of sugars and several serine proteases. There is evidence that single genes located in this region, especially genes encoding serine proteases, are required for efficient colonization of the host. Although C. michiganensis subsp. michiganensis grows mainly in the xylem of tomato plants, no evidence for pronounced genome reduction was found. C. michiganensis subsp. michiganensis seems to have as many transporters and regulators as typical soil-inhabiting bacteria. However, the apparent lack of a sulfate reduction pathway, which makes C. michiganensis subsp. michiganensis dependent on reduced sulfur compounds for growth, is probably the reason for the poor survival of C. michiganensis subsp. michiganensis in soil.

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“…Huang and Tu (1999) have observed neutral to slightly high pH to be better for growth of bacterial canker pathogen of tomato. Eichenlaub et al, (2006) observed that optimum pH for growth of this bacterium is between 7 and 8. Hence, the results of present investigation corroborate the findings of such earlier studies.…”

Section: Effect Of Different Ph Levels On the Growth Of Different Isomentioning

confidence: 90%

“…Amongst different temperature regimes tested, maximum growth of bacterial isolates was observed at 25 0 C. The bacterial growth on the temperatures below and above 25 0 C declined which suggested that 25 0 C is the optimum temperature for the growth of bacterial canker pathogen. Eichenlaub et al, (2006) observed that Clavibacter michiganensis subsp. michiganensis grows optimally at 25°C.…”

Section: Effect Of Different Temperature Regimes On the Growth Of Difmentioning

confidence: 99%

Studies on Bacterial Canker (Clavibacter michiganensis subsp. michiganensis) of Tomato (Solanum lycopersicum)

Bharat¹

2017

Int.J.Curr.Microbiol.App.Sci

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Clavibacter michiganensis, a group of gram-positive phytopathogenic bacteria

Cited by 54 publications

References 99 publications

The endolysins of bacteriophages CMP1 and CN77 are specific for the lysis of Clavibacter michiganensis strains

The endolysins of bacteriophages CMP1 and CN77 are specific for the lysis of Clavibacter michiganensis strains

The Genome Sequence of the Tomato-Pathogenic ActinomyceteClavibacter michiganensissubsp.michiganensisNCPPB382 Reveals a Large Island Involved in Pathogenicity

Studies on Bacterial Canker (Clavibacter michiganensis subsp. michiganensis) of Tomato (Solanum lycopersicum)

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