Clauraila E from the roots of Clausena harmandiana and antifungal activity against Pythium insidiosum

Abstract

A new carbazole alkaloid named clauraila E (1) together with 8 known compounds were isolated from the methanol extract of the roots of Clausena harmandiana. All compounds were evaluated for antifungal activity against Pythium insidiosum using disc diffusion assay. Pythium insidiosum is a fungus-like microorganism, for which antifungals available now are not effective. It was found that compounds 3, 6, 7 and 9 could inhibit the mycelia growth of P. insidiosum. The results show convincingly that they may be lead to compounds for the development of probiotic or novel antifungal drugs.

In vitro activity of carvacrol and thymol combined with antifungals or antibacterials against Pythium insidiosum

“…Prior studies demonstrated the susceptibility of P. insidiosum to antifungal agents [4,9,10,30], antibiotics [20,21,23] 1 and compounds derived from plants and fungi [16,[34][35][36] in vitro. However, tests of drug combinations were performed mainly with antifungal agents, which showed synergistic interactions of 11.8% to 46.7%, particularly when TRB was combined with other antifungal drugs [3,4,9,10].…”

New lignan esters from Alyxia schlechteri and antifungal activity against Pythium insidiosum

“…In continuation of our research on bioactive compounds from medicinal plants [5], we have intensively screened the natural compounds against Pythium insidiosum [6]. P. insidiosum is not a true fungus because its cell walls do not contain ergosterol which is the target for antifungal agents and in addition, it is closely related to diatoms and golden-brown algae [7].…”

Pythium insidiosum: inhibitory effects of propolis and geopropolis on hyphal growth

“… 24 In Thailand, the disease is endemic for humans and the effects of traditional medicinal plants ( Alyxia schlechteri and Clausena harmandiana roots) were investigated, showing that some compounds were able to inhibit the mycelia growth of the pathogen. 25 , 26 …”