Claudin-11 expression and localisation is regulated by androgens in rat Sertoli cells in vitro

Kaitu’u-Lino, Tu’uhevaha J; Sluka, Pavel; Foo, Caroline F H; Stanton, Peter G.

doi:10.1530/rep-06-0385

Cited by 125 publications

(127 citation statements)

References 73 publications

(98 reference statements)

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“…The dissociation of ZO-1 from transmembrane TJ has also been shown to be associated with barrier regulation in the gut (Pappenheimer 1987, Kawkitinarong et al 2004, and a similar mechanism of barrier regulation may be occurring in the testis. Whereas androgens have been shown to regulate TJ proteins in mouse and rat models (Florin et al 2005, Meng et al 2005, Kaitu'u-Lino et al 2007, our data reported here demonstrate that FSH is also able to regulate TJ protein localisation and function in the hamster, and thus it appears that there is a differential response to gonadotrophins at testicular TJs across species.…”

Section: Discussioncontrasting

confidence: 51%

“…Evidence indicates that androgens up-regulate claudin-3 mRNA and protein (Meng et al 2005), and claudin-11 mRNA (Florin et al 2005) and protein localisation in the rodent (Kaitu'u-Lino et al 2007). Thus, we hypothesised that the natural suppression of gonadotrophins in the SD Djungarian hamster would result in decreased tight junction mRNA levels, protein organisation and BTB functionality.…”

Section: Discussionmentioning

confidence: 99%

“…Both follicle-stimulating hormone (FSH) and testosterone increase the function of rat Sertoli cell TJs in vitro (Janecki et al 1991, Kaitu'u-Lino et al 2007 in part by up-regulating claudin-11 mRNA and protein (Kaitu'u-Lino et al 2007), although FSH has also been found to down-regulate claudin-11 mRNA expression in cultured mouse Sertoli cells (Hellani et al 2000). In vivo, selective knockout of the Sertoli cell androgen receptor in mice results in a limited reduction in claudin-11 protein (Tan et al 2005(Tan et al 2005, but a substantial tenfold decrease in claudin-3 gene transcription (Meng et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Regulation of testicular tight junctions by gonadotrophins in the adult Djungarian hamster in vivo

Tarulli¹,

Meachem²,

Schlatt³

et al. 2008

Reproduction

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This study aimed to assess the effect of gonadotrophin suppression and FSH replacement on testicular tight junction dynamics and blood-testis barrier (BTB) organisation in vivo, utilising the seasonal breeding Djungarian hamster. Confocal immunohistology was used to assess the cellular organisation of tight junction proteins and real-time PCR to quantify tight junction mRNA. The effect of tight junction protein organisation on the BTB permeability was also investigated using a biotin-linked tracer. Tight junction protein (claudin-3, junctional adhesion molecule (JAM)-A and occludin) localisation was present but disorganised after gonadotrophin suppression, while mRNA levels (claudin-11, claudin-3 and occludin) were significantly (two-to threefold) increased. By contrast, both protein localisation and mRNA levels for the adaptor protein zona occludens-1 decreased after gonadotrophin suppression. FSH replacement induced a rapid reorganisation of tight junction protein localisation. The functionality of the BTB (as inferred by biotin tracer permeation) was found to be strongly associated with the organisation and localisation of claudin-11. Surprisingly, JAM-A was also recognised on spermatogonia, suggesting an additional novel role for this protein in trans-epithelial migration of germ cells across the BTB. It is concluded that gonadotrophin regulation of tight junction proteins forming the BTB occurs primarily at the level of protein organisation and not gene transcription in this species, and that immunolocalisation of the organised tight junction protein claudin-11 correlates with BTB functionality.

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Section: Discussioncontrasting

confidence: 51%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Regulation of testicular tight junctions by gonadotrophins in the adult Djungarian hamster in vivo

Tarulli¹,

Meachem²,

Schlatt³

et al. 2008

Reproduction

Self Cite

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“…Until recently, Kaitu'u-Lino et al (2007) have demonstrated that testosterone regulates claudin-11 expression and promotes the localization of claudin-11 and occludin at Sertoli-Sertoli cell interfaces. Along this line, studies by Meng et al (2005) have shown that testosterone positively regulates the expression of claudin-3 in mice, which is believed to be a transient component of newly-formed tight junctions at the BTB.…”

Section: Testosteronementioning

confidence: 99%

“…However, in claudin-11 expression, rat and mouse Sertoli cells responded to FSH treatment oppositely. It was found that FSH could partially stimulate claudin-11 mRNA in cultured rat Sertoli cells, and it inhibited the expression of claudin-11 mRNA in mouse Sertoli cells (Hellani et al 2000, Kaitu'u-Lino et al 2007. Despite the fact that the importance of FSH in regulating junction restructuring has been known for a long time, the molecular mechanism explaining how FSH exerts its effect remains enigmatic.…”

Section: Fsh and Estrogenmentioning

confidence: 99%

Molecular mechanisms by which hormones and cytokines regulate cell junction dynamics in the testis

Lui

Lee²

2009

Journal of Molecular Endocrinology

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Hormones and cytokines are known to regulate cellular functions in all tissues including testis. These two groups of biomolecules exert a broad spectrum of effects on various aspects of spermatogenesis. Among them, one of the regulatory effects on spermatogenesis is to modulate cell junction restructuring between Sertoli cells and between Sertoli and germ cells in the seminiferous epithelium. The restructuring of cell junctions is crucial to enable the migration of germ cells along the seminiferous epithelium from the basement membrane towards the tubular lumen, and at the same time for their attachment to Sertoli cells for support. This review will summarize the recent findings that focus on the role of hormones (FSH and testosterone) and cytokines (transforming growth factor-bs and tumor necrosis factor-a) on cell junction restructuring in the testis and the molecular mechanisms.

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Hepatocyte growth factor modulates Sertoli–Sertoli tight junction dynamics

Catizone

Ricci

Galdieri

2008

Journal Cellular Physiology

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In mammalian testes Sertoli cells form tight junctions whose function is fundamental for the maintenance of a normal spermatogenesis. Hepatocyte growth factor (HGF) is a cytokine influencing the cellular tight junctions either in normal or in tumor cells. We have previously demonstrated that HGF is expressed in the rat testis and influences many functional activities of somatic and germ cells. We now report that HGF decreases the levels of testicular occludin and influences the position of the molecule in the tight junctions as demonstrated by confocal microscopy analysis. In fact in the presence of the factor occludin was mainly localized in the suprabasal region of the tubules whereas in its absence occludin was prevalently localized in the basal region. Occludin production is known to be regulated by different cytokines including TGFbeta. We have investigated the role of HGF in the regulation of the levels of TGFbeta and we report that HGF significantly increases the amount of the active fraction of the factor without affecting the amount of the total TGFbeta. Urokinase type plasminogen activator (uPA) is closely related with the tight junctions and is one of the molecules able to activate the inactive TGF-beta. We found that HGF significantly increases the amount of uPA present in the testis suggesting that HGF regulates the amount of active TGFbeta via uPA levels. In conclusion we report that in the testis HGF regulates Sertoli-Sertoli tight junctions inducing a reduction and redistribution of occludin possibly modulating the levels of uPA and active TGFbeta.

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Claudin-11 expression and localisation is regulated by androgens in rat Sertoli cells in vitro

Cited by 125 publications

References 73 publications

Regulation of testicular tight junctions by gonadotrophins in the adult Djungarian hamster in vivo

Regulation of testicular tight junctions by gonadotrophins in the adult Djungarian hamster in vivo

Molecular mechanisms by which hormones and cytokines regulate cell junction dynamics in the testis

Hepatocyte growth factor modulates Sertoli–Sertoli tight junction dynamics

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