Claudin-1, -3 and -4 proteins and mRNA expression in benign and malignant breast lesions: a research study

Tőkés, Anna-Mária; Kulka, Janina; Paku, Sándor; Simonyi, Ágnes; Páska, Csilla; Novák, Pál Kaposi; Szilák, László; Kiss, András; Krisztina, Bögi; Schaff, Zsuzsa

doi:10.1186/bcr983

Cited by 211 publications

(120 citation statements)

References 32 publications

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“…Compared to human mammary tissue, the same CLDNs are present in canine mammary epithelial cells (Tőkés et al, 2005). This study provided further evidence that CLDN proteins are ancient, conservative molecules that are preserved throughout different species.…”

Section: Acta Veterinaria Hungarica 56 2008supporting

confidence: 51%

Expression and localisation of claudin-1,-2,-3,-4,-5,-7 and-10 proteins in the normal canine mammary gland

Jakab

Halász

Szász

et al. 2008

Acta Veterinaria Hungarica

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The recently identified claudins are dominant components of tight junctions, responsible for cell adhesion, polarity and paracellular permeability. Certain claudins have been shown to have relevance in tumour development. The aim of the present study was to analyse the expression of claudin-1, -2, -3, -4, -5, -7 and -10 in normal canine mammary glands. Samples from the inguinal mammary regions of 20 non-castrated, 1-13 years old female dogs were studied. Immunohistochemical analysis was performed on conventional specimens and tissue microarrays. The results of the immunohistochemical reactions detecting claudins in tissue sections were photodocumented. The immunoreactivity of claudins was quantitatively analysed on digital images using Leica QWin morphometry software. Intense membranous immunolabelling was found for claudin-1, -3 and -7, intense membranous with non-granular cytoplasmic immunolabelling for claudin-2, moderate membranous immunolabelling for claudin-4 and -5, and weak membranous immunolabelling for claudin-10. The occurrence of tight junctions was confirmed by ultrathin section electron microscopy. The available data suggested that claudins might be proteins preserved throughout the evolution of mammals. The results of our study support the concept that they are indeed preserved, since the same type of claudins, in identical distribution, could be detected in our canine mammary tissue samples as could be found in human mammary tissue.

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Section: Acta Veterinaria Hungarica 56 2008supporting

confidence: 51%

Expression and localisation of claudin-1,-2,-3,-4,-5,-7 and-10 proteins in the normal canine mammary gland

Jakab

Halász

Szász

et al. 2008

Acta Veterinaria Hungarica

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“…CLAUDIN-1 expression is altered in several human malignancies (Resnick et al 2005, Tokes et al 2005, Chao et al 2009) and subcellular expression of CLAUDIN-1 has been linked with tumour invasiveness and an advanced tumour stage (Dhawan et al 2005, Kinugasa et al 2012, Eftang et al 2013. We asked whether CLAUDIN-1 is relevant for the biological behaviour of human follicular thyroid cancer.…”

Section: Discussionmentioning

confidence: 99%

“…In tumor progression, tight junction assembly is often disrupted resulting in the loss of cell membrane localized tight junction proteins (Resnick et al 2005, Tokes et al 2005. For CLAUDIN-1, an altered protein expression as well as subcellular localization has been observed in common human malignancies including colon, lung and breast cancer and melanoma (Tokes et al 2005, Chao et al 2009, Nemeth et al 2010. Furthermore, subcellular CLAUDIN-1 expression has been associated with a more aggressive tumour behaviour (Miwa et al 2001).…”

Section: Introductionmentioning

confidence: 99%

“…From the currently known 27 CLAU-DIN members, CLAUDIN-1 is assumed to be the most important protein responsible for the paracellular barrier integrity of epithelial cells (Gonzalez-Mariscal et al 2003). In tumor progression, tight junction assembly is often disrupted resulting in the loss of cell membrane localized tight junction proteins (Resnick et al 2005, Tokes et al 2005. For CLAUDIN-1, an altered protein expression as well as subcellular localization has been observed in common human malignancies including colon, lung and breast cancer and melanoma (Tokes et al 2005, Chao et al 2009, Nemeth et al 2010.…”

Section: Introductionmentioning

confidence: 99%

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The impact of CLAUDIN-1 on follicular thyroid carcinoma aggressiveness

Zwanziger¹,

Badziong²,

Ting³

et al. 2015

Endocrine-Related Cancer

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CLAUDIN-1 belongs to the family of transmembrane tight junction proteins tightening the paracellular cleft of epithelial cells. In human malignancies, CLAUDIN-1 is often dysregulated and located in subcellular compartments, particularly in the nucleus where it may influence cellular behaviour. Here, we studied CLAUDIN-1 in relation to the biological characteristics of follicular thyroid carcinoma (FTC). CLAUDIN-1 immuno-staining showed loss of membrane expression and increased nuclear CLAUDIN-1 localization in FTC metastases. CLAUDIN-1 function was further investigated in two different follicular thyroid carcinoma cell lines: FTC-133 isolated from a regional lymph node metastasis and FTC-238 derived from a lung metastasis. In both cell lines CLAUDIN-1 expression was demonstrated in the cell nuclei with a significantly higher protein expression in FTC-238 compared to FTC-133 cells. Interestingly, in vitro scratch assay revealed enriched nuclear CLAUDIN-1 expression near the scratch. Furthermore, the increase of the pathogenic character of FTC-133 cells by RASV12 transfection was associated with elevated CLAUDIN-1 expression and enhanced cell migration, invasion and proliferation. Likewise over-expression of nuclear CLAUDIN-1 in FTC-133 cells resulted in increased cell migration and invasion. Conversely, CLAUDIN-1 downregulation in FTC-238 cells by siRNA resulted in decreased cell migration and invasion and was accompanied by reduced phosphoPKC expression. Moreover, activation and inhibition of PKC resulted in CLAUDIN-1 up-and downregulation in FTC cells respectively. These data suggest an impact of CLAUDIN-1 on follicular thyroid carcinoma aggressiveness, which could potentially be influenced by PKC activity.

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“…Previous studies have identified the expression of claudins in several cancer types, including breast, pancreatic, liver and esophageal cancer (10,(29)(30)(31)(32)(33). Claudin-1 expression was reported be attenuated in breast cancer as well as colon cancer (31,34,35). In addition, the expression levels of claudin-1, claudin-3, claudin-4 and claudin-5 were all significantly decreased in diffuse adenocarcinoma and were essential for determining the phenotype and loose cohesion of cells in diffuse gastric carcinoma (15).…”

Section: Claudin-1 Claudin-4 ----------------------------------------mentioning

confidence: 95%

Claudin-1, but not claudin-4, exhibits differential expression patterns between well- to moderately-differentiated and poorly-differentiated gastric adenocarcinoma

et al. 2015

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Abstract.Claudins are members of a large family of transmembrane proteins, which are essential in the formation of tight junctions and have previously been associated with the process of tumor progression. Studies have reported the aberrant expression of claudin-1 and claudin-4 in numerous types of cancer. The present study aimed to investigate the expression of claudin-1 and claudin-4 in gastric adenocarcinoma tissue. Surgically resected gastric adenocarcinoma tissue specimens were obtained from 94 patients. Protein expression levels of claudin-1 and claudin-4 were determined using immunohistochemical staining; the association between claudin-1 or claudin-4 expression and various clinicopathological parameters were then analyzed. In gastric adenocarcinoma specimens, the expression rates of claudin-1 and claudin-4 were 43.6 and 87.2%, respectively. Claudin-1 expression demonstrated a significant correlation with histological type (P<0.01) and was significantly higher in well-to moderately-differentiated gastric adenocarcinomas compared with poorly-differentiated tumors. However, no correlation was observed between claudin-4 expression in adenocarcinoma and clinicopathological parameters. In conclusion, downregulation of claudin-1 expression in poorly-differentiated gastric adenocarcinoma may be involved in the biological transformation of tumors. The present findings suggested that claudin-1 may be an important protein associated with histological type and therefore may have potential for use as a prognostic marker for gastric adenocarcinoma. Further studies are required to elucidate the precise mechanism of claudin expression and its involvement in tumor progression.

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Claudin-1, -3 and -4 proteins and mRNA expression in benign and malignant breast lesions: a research study

Abstract: Introduction We compared levels of protein and mRNA expression of three members of the claudin (CLDN) family in malignant breast tumours and benign lesions.

Cited by 211 publications

References 32 publications

Expression and localisation of claudin-1,-2,-3,-4,-5,-7 and-10 proteins in the normal canine mammary gland

Expression and localisation of claudin-1,-2,-3,-4,-5,-7 and-10 proteins in the normal canine mammary gland

The impact of CLAUDIN-1 on follicular thyroid carcinoma aggressiveness

Claudin-1, but not claudin-4, exhibits differential expression patterns between well- to moderately-differentiated and poorly-differentiated gastric adenocarcinoma

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