Clathrin meets caveolae: fuse or not?

Chen, Chunying

doi:10.1007/s11434-015-0909-6

Cited by 6 publications

(3 citation statements)

References 9 publications

(18 reference statements)

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“…Being an important basic physiological process, endocytosis is a major mechanism by which cells uptake nutrients/signaling molecules and regulates the fate of receptors . The endocytic system is comprised of a highly dynamic network of vesicles and endosomes; thus, real-time monitoring of the communications and interaction between endosomal vesicles is important for a better understanding of the function of endocytosis and intracellular trafficking . The endocytic process is a spatiotemporal succession of events, including transporting internalized cargos from the membrane to the trafficking vesicles, targeting vesicles to endosomes, and then to lysosomes .…”

Section: Resultsmentioning

confidence: 99%

“…2 The endocytic system is comprised of a highly dynamic network of vesicles and endosomes; thus, real-time monitoring of the communications and interaction between endosomal vesicles is important for a better understanding of the function of endocytosis and intracellular trafficking. 46 The endocytic process is a spatiotemporal succession of events, including transporting internalized cargos from the membrane to the trafficking vesicles, targeting vesicles to endosomes, and then to lysosomes. 47 There are two major endocytic pathways, clathrin-mediated and caveolae-mediated pathways.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Fluorescent Polymer Dot-Based Multicolor Stimulated Emission Depletion Nanoscopy with a Single Laser Beam Pair for Cellular Tracking

Ruan

Dong

et al. 2020

Anal. Chem.

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Stimulated emission depletion (STED) nanoscopy provides subdiffraction resolution while preserving the benefits of fluorescence confocal microscopy in live-cell imaging. However, there are several challenges for multicolor STED nanoscopy, including sophisticated microscopy architectures, fast photobleaching, and cross talk of fluorescent probes. Here, we introduce two types of nanoscale fluorescent semiconducting polymer dots (Pdots) with different emission wavelengths: CNPPV (poly[2-methoxy-5-(2-ethylhexyloxy)-1,4-(1-cyanovinylene-1,4-phenylene)]) Pdots and PDFDP (poly[{9,9-dihexyl-2,7-bis(1-cyanovinylene)fluorene}-alt-co-{2,5-bis (N,N′-diphenylamino)-1,4-phenylene}]) Pdots, for dual-color STED bioimaging and cellular tracking. Besides bright fluorescence, strong photostability, and easy bioconjugation, these Pdots have large Stokes shifts, which make it possible to share both excitation and depletion beams, thus requiring only a single pair of laser beams for the dual-color STED imaging. Long-term tracking of cellular organelles by the Pdots has been achieved in living cells, and the dynamic interaction of endosomes derived from clathrin-mediated and caveolae-mediated endocytic pathways has been monitored for the first time to propose their interaction models. These results demonstrate the promise of Pdots as excellent probes for live-cell multicolor STED nanoscopy.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Fluorescent Polymer Dot-Based Multicolor Stimulated Emission Depletion Nanoscopy with a Single Laser Beam Pair for Cellular Tracking

Ruan

Dong

et al. 2020

Anal. Chem.

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“…Transforming growth factor b (TGF-b) plays an important role in regulating a broad range of biological processes (e.g., growth, migration, proliferation, and differentiation), and is associated with a number of human diseases, including cancer, tissue fibrosis and cardiovascular diseases [1][2][3][4]. Since the signaling of TGF-b is initiated through the binding with its specific membrane receptor TGF-b type II (TbRII) [2,5], much effort has been made to understand the diffusion dynamics and activation process of TbRII using single-molecule techniques [6][7][8][9][10], which can provide detailed information on the spatial and temporal biomolecular heterogeneity that is hidden in the conventional ensemble measurements [11,12].…”

Section: Introductionmentioning

confidence: 99%