Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization and vascular tissue formation in Arabidopsis

Mazur, Ewa; Gallei, Michelle; Adamowski, Maciek; Han, Huibin; Robert, Hélène S.; Friml, Jiřı́

doi:10.1016/j.plantsci.2020.110414

Cited by 25 publications

(25 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5h ), vascular tissue regeneration and formation induced by wounding or external auxin sources 59 . These data strengthen the previously established connection 59 between subcellular trafficking and their dynamic auxin regulations at the level of individual cells and long-term auxin-induced canalization processes across tissues.…”

Section: Resultsmentioning

confidence: 99%

Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization

et al. 2020

Self Cite

View full text Add to dashboard Cite

Directional transport of the phytohormone auxin is a versatile, plant-specific mechanism regulating many aspects of plant development. The recently identified plant hormones, strigolactones (SLs), are implicated in many plant traits; among others, they modify the phenotypic output of PIN-FORMED (PIN) auxin transporters for fine-tuning of growth and developmental responses. Here, we show in pea and Arabidopsis that SLs target processes dependent on the canalization of auxin flow, which involves auxin feedback on PIN subcellular distribution. D14 receptor-and MAX2 F-box-mediated SL signaling inhibits the formation of auxin-conducting channels after wounding or from artificial auxin sources, during vasculature de novo formation and regeneration. At the cellular level, SLs interfere with auxin effects on PIN polar targeting, constitutive PIN trafficking as well as clathrin-mediated endocytosis. Our results identify a non-transcriptional mechanism of SL action, uncoupling auxin feedback on PIN polarity and trafficking, thereby regulating vascular tissue formation and regeneration.

show abstract

Section: Resultsmentioning

confidence: 99%

Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…This, in turn, is an essential pre-requisite of auxin canalization, a process allowing for the self-organized formation of polarized auxin channels during many developmental processes (Wabnik et al, 2010(Wabnik et al, , 2011. Indeed, genetic and pharmacological manipulations revealed the crucial importance of auxin-regulated trafficking for auxin effect on PIN polarity and for formation of auxin transporting channels (Mazur et al, 2020a;Hajný et al, 2020).…”

Section: Auxin-mediated Myosin XI Phosphorylation In Pin1 Repolarization In Rootsmentioning

confidence: 99%

“…The classical auxin canalization hypothesis proposes auxin feedback regulation of directional auxin transport as the key part underlying self-organizing aspects of plant development, such as vasculature formation during spontaneously arising leaf venation patterns (Scarpella et al, 2006) and flexible regeneration of vasculature around wounding sites (Sauer et al, 2006;Mazur et al, 2016Mazur et al, , 2020a.…”

Section: Auxin-mediated Myosin XI Phosphorylation In Auxin Canalization-mediated Vasculature Formation and Regenerationmentioning

confidence: 99%

“…This positive feedback between auxin signaling and transport directionality is the key pre-requisite of the so-called auxin canalization (Sachs, 1981). The auxin canalization hypothesis proposes a mechanism for unique, self-organizing aspects of plant development such as the establishment of the apical/basal body axis during embryogenesis (Robert et al, 2013(Robert et al, , 2018, flexible formation of vascular strands during organ formation (Benková et al, 2003;Heisler et al, 2005;Bhatia et al, 2016), shoot branching (Bennett et al, 2014;, leaf venation (Scarpella et al, 2006;Govindaraju et al, 2020), vasculature regeneration after wounding (Sauer et al, 2006;Mazur et al, 2016Mazur et al, , 2020aHajný et al, 2020) and shoot gravitropic bending termination (Rakusová et al, 2016(Rakusová et al, , 2019Han et al, 2020). During canalization, auxin triggers coordinated PIN polarization to gradually establish directional auxin transport channels between auxin source and sink (Wabnik et al, 2010(Wabnik et al, , 2011Prát et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…During canalization, auxin triggers coordinated PIN polarization to gradually establish directional auxin transport channels between auxin source and sink (Wabnik et al, 2010(Wabnik et al, , 2011Prát et al, 2018). Genetic and cell biological studies suggest that the auxin effect on PIN endocytic trafficking is central for PIN polarization and canalization (Sauer et al, 2006;Mazur et al, 2020a;. Nonetheless, auxin signaling mechanisms and downstream cellular processes, by which auxin regulates both PIN polarity and subcellular trafficking crucial for the self-organizing canalization processes, still remain elusive.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Rapid auxin-mediated phosphorylation of Myosin regulates trafficking and polarity in Arabidopsis

Han

Verstraeten

Roosjen

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

The signaling molecule auxin controls plant development through a well-known transcriptional mechanism that regulates many genes. However, auxin also triggers cellular responses within seconds or minutes, and mechanisms mediating such fast responses have remained elusive. Here, we identified an ultrafast auxin-mediated protein phosphorylation response in Arabidopsis roots that is largely independent of the canonical TIR1/AFB receptors. Among targets of this novel response are Myosin XI and its adaptor protein MadB2. We show that their auxin-mediated phosphorylation regulates trafficking and polar, subcellular distribution of PIN auxin transporters. This phosphorylation-based auxin signaling module is indispensable during developmental processes that rely on auxin-mediated PIN repolarization, such as termination of shoot gravitropic bending or vasculature formation and regeneration. Hence, we identified a fast, non-canonical auxin response targeting multiple cellular processes and revealed auxin-triggered phosphorylation of a myosin complex as the mechanism for feedback regulation of directional auxin transport, a central component of auxin canalization, which underlies self-organizing plant development.

show abstract

AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells

et al. 2021

View full text Add to dashboard Cite

Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization and vascular tissue formation in Arabidopsis

Cited by 25 publications

References 36 publications

Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization

Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization

Rapid auxin-mediated phosphorylation of Myosin regulates trafficking and polarity in Arabidopsis

AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells

Contact Info

Product

Resources

About