Clastogenic and mutagenic effects of bisphenol A: An endocrine disruptor

Tiwari, D. C.; Kamble, Jyoti; Chilgunde, Swapnil; Patil, Pramod B.; Maru, Girish B.; Kawle, Dhananjay; Bhartiya, Uma S.; Joseph, Lebana J.; Vanage, Geeta

doi:10.1016/j.mrgentox.2011.12.023

Cited by 115 publications

(78 citation statements)

References 56 publications

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“…22,23,24,26 Here, we discuss data obtained from our in vitro study conducted in order to establish the clastogenic and aneugenic potential of BP-A on human cultured lymphocytes.…”

Section: Discussionmentioning

confidence: 99%

Clastogenic effects of bisphenol A on human cultured lymphocytes

et al. 2017

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Abstract:Bisphenol-A is an endocrine disrupting compound widely used in the production of polycarbonate plastics and epoxy resins. It is ubiquitously present in the environment, mostly in aquatic environments, with consequent risks to the health of aquatic organisms and humans. In the present study we analysed the cytogenetic effects of bisphenol-A on human lymphocytes using in vitro chromosomal aberrations and micronuclei assays. Lymphocyte cultures were exposed to five different concentrations of BP-A (0.20, 0.10, 0.05, 0.02 and 0.01 µg/mL) for 24 h (for chromosomal aberrations test) and 48 h (for micronuclei test). The concentration of 0.05 µg/mL represents the reference dose established by United States Environmental Protection Agency; 0.02 µg/mL represents the higher concentration of unconjugated BP-A found in human serum and 0.01 µg/mL represents the tolerable daily intake established by European Union. Data obtained with both assays showed significant genotoxic effects of the bisphenol-A at concentrations of 0.20, 0.10 and 0.05 µg/mL, whereas at the concentration of 0.02 µg/mL we observed only a significant increase in the micronuclei frequency. Finally, at the concentration of 0.01 µg/mL no cytogenetic effects were observed, indicating this latter as a more tolerable concentration for human health with respect to 0.05 µg/mL, the reference dose established by US EPA.

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“…22,23,24,26 Here, we discuss data obtained from our in vitro study conducted in order to establish the clastogenic and aneugenic potential of BP-A on human cultured lymphocytes.…”

Section: Discussionmentioning

confidence: 99%

Clastogenic effects of bisphenol A on human cultured lymphocytes

et al. 2017

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“…The increase in DNA strand breaks was signifi cant as soon as after three hours of exposure and increased even further up to hour 24, which is in agreement with our study. When applied in vivo in rats, BPA induced micronucleus formation and structural chromosome aberrations in bone marrow as well as DNA damage in lymphocytes (46). Tiwari et al (46) also observed increased plasma levels of 8-hydroxydeoxyguanosine, an increase in lipid peroxidation, and a decrease in glutathione activity in the liver, suggesting that oxidative stress could be one of the mechanisms of BPA genotoxicity.…”

Section: Bisphenol-induced Dna Strand Breaks In Hepg2 Cellsmentioning

confidence: 97%

Mutagenicity and DNA Damage of Bisphenol a and its Structural Analogues in Hepg2 Cells

Fic

Žegura

Dolenc

et al. 2013

Archives of Industrial Hygiene and Toxicology

101

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Environmental oestrogen bisphenol A (BPA) and its analogues are widespread in our living environment. Because their production and use are increasing, exposure of humans to bisphenols is becoming a signifi cant issue. We evaluated the mutagenic and genotoxic potential of eight BPA structural analogues (BPF, BPAF, BPZ, BPS, DMBPA, DMBPS, BP-1, and BP-2) using the Ames and comet assay, respectively. None of the tested bisphenols showed a mutagenic effect in Salmonella typhimurium strains TA98 and TA100 in either the presence or absence of external S9-mediated metabolic activation (Aroclor 1254-induced male rat liver). Potential genotoxicity of bisphenols was determined in the human hepatoma cell line (HepG2) at non-cytotoxic concentrations (0.1 μmol L -1 to 10 μmol L -1 ) after 4-hour and 24-hour exposure. In the comet assay, BPA and its analogue BPS induced signifi cant DNA damage only after the 24-hour exposure, while analogues DMBPS, BP-1, and BP-2 induced a transient increase in DNA strand breaks observed only after the 4-hour exposure. BPF, BPAF, BPZ, and DMBPA did not induce DNA damage.

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“…These photoinitiators are used in printing. 1,18) Therefore, we propose that 1-HCHPK is derived from the printing ink on injection packages and has different migration behavior in different injection components.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, bisphenol A is a well-known endocrine disruptor, which represents a major toxicological and public health concern due to its widespread exposure to humans. 1) Nonylphenol is also a persistent endocrine disruptor.…”

mentioning

confidence: 99%

Quantitation and Human Monocyte Cytotoxicity of the Polymerization Agent 1-Hydroxycyclohexyl Phenyl Ketone (Irgacure 184) from Three Brands of Aqueous Injection Solution

Yamaji

Kawasaki

Yoshitome

et al. 2012

Biological & Pharmaceutical Bulletin

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In this study, levels of the photoinitiator 1-hydroxycyclohexyl phenyl ketone (1-HCHPK) in aqueous injection solutions were analyzed by GC-MS. In our previous studies, photoinitiators such as 2-methyl-4′-(methylthio)-2-morpholinopropiophenone (MTMP) were detected in intravenous (i.v.) injection bag solution, and they were found to be cytotoxic to human monocytes. Therefore, we hypothesized that 1-HCHPK might display similarly cytotoxicity. The purpose of this study was to quantitate the amount of contaminants from plastic containers such as those used for peripheral parenteral nutrition and to determine the cytotoxicity of such extracts on human monocytes. The sample extraction procedure for GC-MS analysis involved a liquid-phase extraction. The solvent was evaporated under a stream of nitrogen at 50°C to yield a residue, which was dissolved in n-hexane and injected into a GC-MS. Normal human peripheral blood mononuclear cells (PBMC), isolated from the buffy coat by centrifugation, were suspended in RPMI 1640 medium supplemented with 10% (v/v) heat-inactivated fetal calf serum. In the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability assay, cells (1 10 4 ) were treated with 1-HCHPK for 24 h or 48 h at 37°C. From the GC-MS analysis, 6.13-8.32 µg/mL of 1-HCHPK was found in 20 mL vials of water for injection solution. In the MTT assay, 1-HCHPK decreased cell viability for both the 24 h and 48 h incubation periods. In conclusion, our findings suggest that 1-HCHPK could promote adverse events in patients. Future studies will clarify the possible health risks of photoinitiator accumulation in human cells.

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Clastogenic and mutagenic effects of bisphenol A: An endocrine disruptor

Cited by 115 publications

References 56 publications

Clastogenic effects of bisphenol A on human cultured lymphocytes

Clastogenic effects of bisphenol A on human cultured lymphocytes

Mutagenicity and DNA Damage of Bisphenol a and its Structural Analogues in Hepg2 Cells

Quantitation and Human Monocyte Cytotoxicity of the Polymerization Agent 1-Hydroxycyclohexyl Phenyl Ketone (Irgacure 184) from Three Brands of Aqueous Injection Solution

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