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Pandita, Sangeeta; Passey, Sarita

doi:10.1007/s12045-010-0124-3

Cited by 4 publications

(2 citation statements)

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“…Moreover, our results are presented with increased clarity and accessibility, enhancing the usability of microscale functional group analysis. In this study, the method has been applied to detect several organic functional groups, − based on a microscale analysis. A 32 cell well plate was used and drops of an organic sample solution with the same or different functional groups were added to the reagent to observe any changes (Table and Figures S1–S12).…”

Section: Methodsmentioning

confidence: 99%

Green Approaches for Efficient Functional Group Analysis of Organic Compounds

Othman,

Omer,

Mohammad

et al. 2024

J. Chem. Educ.

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Functional group analysis is an essential part of the curriculum for identifying unknown organic compounds. However, traditional methods of analyzing functional groups use excess reagents and generate a large amount of waste material that is difficult to dispose of. This work is focused on alternative methods for detecting functional groups such as carboxylic acid, ester, alkene, alcohol, aldehyde, ketone, phenol, anhydride, amine, carbohydrate, and amino acid. The experiments use the most common 44 organic compounds and apply 17 chemical tests with little modification and considerably less volume (few drops) of conventional reagents on a grooved tile. In addition, this work provides some tests that can give different positive results for different functional groups (i.e., the sodium nitroprusside test can be used for the detection of ketones and amines with different results, and the ceric ammonium nitrate test can be used for the detection of alcohols, phenols, and carbohydrates). The procedures adopted are in accordance with the principles of green chemistry: consumption of fewer solvents and minimizing waste and time. Therefore, these experiments provide an efficient, safe and economical approach for functional group investigation. The success of the tests is valid for general organic compounds.

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Section: Methodsmentioning

confidence: 99%

Green Approaches for Efficient Functional Group Analysis of Organic Compounds

Othman,

Omer,

Mohammad

et al. 2024

J. Chem. Educ.

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“…Supernatant was collected which contained intracellular enzyme. Pretreated wheat sample was used as substrate for cellulolytic enzymes as method describedbyElliston and Collins [9].Ceric Ammonium nitrate Assay was carried out by the method described byPanditaand Passey [10].…”

Section: Methodsmentioning

confidence: 99%

Feedback inhibition of cellulolytic enzymes during saccharification of cellulosic biomass in order to enhance the productivity

Razzaq¹

2018

PAB

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Feedback inhibition of cellulolytic enzymes during saccharification of cellulosic biomass in order to enhance the productivity. AbstractCurrent study revealed inhibitory effect of released end products on the activity of cellulolytic enzymes, both natural and synthetic substrates were compared comparatively.Genetically modified Escherichia coli containing the genes of Thermotogapetrophila for the production of endoglucanse, exoglucanse and ß-glucosidase were provided for this research work production of bioenergy from plant biomas. These clones were named as N1, X3 and G1.These cultures were maintained by streaking these on ampicillin containing LB agar plates and were stored by placing them in cold cabinet at 4°C. Sub culturing. It was demonstrated that accumulation of the end products upto a certain concentration with respect to the specific enzyme loading (U/mL), led to the inactivation of the enzymes in saccharification mixture. Overall percentage saccharification of natural and synthetic substrate due to feedback inhibition of sequentially added cellulolytic enzymes was found 23.6% and 29.4% respectively. Variable concentrations of cellulolytic enzymes were added sequentially in order to overcome the inhibitory effect of the released end products during saccharification. This resulted in a significant increase in percentage saccharification of both natural and synthetic substrate upto 33.6% and 34.5% respectively.

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Safe and Green A Convenient Laboratory Preparation of Acetanilide*

Passey¹,

Arora²

2022

Reson

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Classroom

Cited by 4 publications

References 1 publication

Green Approaches for Efficient Functional Group Analysis of Organic Compounds

Green Approaches for Efficient Functional Group Analysis of Organic Compounds

Feedback inhibition of cellulolytic enzymes during saccharification of cellulosic biomass in order to enhance the productivity

Safe and Green A Convenient Laboratory Preparation of Acetanilide*

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