A modular experiment for upper-level
undergraduate students is
presented, featuring the product cycle and process evaluating the
quality and quantity of biodiesel obtained from a microalgae feedstock
grown under carbon-supplemented and nutrient-deprived conditions.
Batch reactors are used to cultivate and harvest microalgae, which
undergo acid-catalyzed in situ transesterification
to produce biodiesel. Methods are presented to quantify yield and
analyze products via gas chromatography–mass spectroscopy.
With an awareness of the limited time available in a teaching laboratory,
this experiment was designed and successfully implemented in an undergraduate
biochemistry course over four sessions. Reflux reactions were performed
in hermetically sealed microwave vials, eliminating water usage and
reducing glass breakage. Learning objectives were assessed by postcontent
quizzes, and written reports using a rubric based on Bloom’s
taxonomy, results of which indicate a demonstration of the higher-order
cognitive abilities of Evaluation and Analysis. The ability to biochemically
engineer cultivation conditions to influence lipid production gives
this experiment the potential to be offered as a course-embedded undergraduate
research experience (CURE) project. This easily adoptable experiment
offers an engaging opportunity for students to experience the technical
and societal aspects of emerging biofuel technologies.