Classifying amygdala kindling stages using quantitative assessments of extracellular recording of EEG in rats

Jalilifar, Mostafa; Yadollahpour, Ali; Moazedi, Ahmad Ali; Ghotbeddin, Zohreh

doi:10.1016/j.brainresbull.2016.09.012

Cited by 14 publications

(11 citation statements)

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“…In the recently published papers, we identified the main quantitative features of different kindling stages during epileptogenesis ( Jalilifar et al, 2016 ) and then for the main phases of seizure acquisition initial, localized and generalized seizure stages in the amygdale rapid kindling model ( Jalilifar et al, 2017b ) (detailed data not provided here). The main features of kindling were as follows: The kindling acquisition process was accompanied by increase in delta (1–4 Hz) and theta (4–8 Hz) waves in the stages of 3, 4, and 5, compared with the control group.…”

Section: Resultsmentioning

confidence: 99%

“…Since the results confirmed no significant differences in LFB, MFB, and HFB powers of the baseline EEG signals between the experimental groups, it is claimed that the group differences can only account for the seizure activity but not the background EEG. We previously reported increase of delta and theta oscillations with generalization of the kindling process ( Jalilifar et al, 2016 ). In this regard, power spectrum results confirmed that the LFB (delta and theta sub-bands) is dominant with the progression of the kindling acquisition.…”

Section: Discussionmentioning

confidence: 99%

“…Developing quantitative and objective assessments of LFS effects on epileptogenesis using EEG signals can reduce the objective and subjective errors present in the behavioral assessments. In our previous studies we determined the main spectral features of EEG signals in different stages of kindling and also assessed the variations of different EEG based measures during progression of kindling ( Jalilifar et al, 2016 , 2017b ). Our findings along with the findings of previous studies showed significant correlations between variations of specific sub-bands of EEG with different phases of epileptogenesis: We classified EEG signal into different sub-bands whose powers are considered as a synchronization of neural discharge index.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative Analysis of the Antiepileptogenic Effects of Low Frequency Stimulation Applied Prior or After Kindling Stimulation in Rats

et al. 2018

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Background and Objective: Developing quantitative measures based on spectral analysis of electroencephalograph (EEG) recordings of neural activities plays an important role in developing efficient treatments for epilepsy. Such biomarkers can be used for developing open or closed loop approaches for seizure prediction or prevention. This study aims to quantitatively evaluate antiepileptogenic effects of low frequency stimulation (LFS) applied immediately before or after kindling stimulations using spectral power analysis of extracellular EEG in rat.Methods: Nineteen adult rats were used: seven for kindle, six for LFS+Kindle (LFSK) and six for Kindle+LFS (KLFS). Four packages of LFS (1Hz) were applied immediately before or after rapid kindling stimulations. The power spectral densities of afterdischarge (AD) sections of EEG corresponding to different stages of kindling for delta (0–4 Hz), theta (4–8 Hz), alpha (8–12 Hz), beta (12–28 Hz), gamma (28–40 Hz) sub-bands, and theta/alpha ratio were comparatively investigated. Moreover, correlation between AD duration (ADD) and its different frequency components was calculated.Results: Both LFSK and KLFS significantly increased delta and reduced beta and gamma oscillations, compared with kindle group. However, just the reduction in LFSK group was significant. Both protocols increased theta/alpha ratio, but just LFSK showed significant increase (p < 0.05). Although LFSK enhanced theta/alpha ratio more than KLFS, the difference was not statistically significant. Furthermore, strong correlation between each frequency sub band and ADD was not observed in kindle and LFS treated groups (both LFSK and KLFS).Conclusion: Although behavioral assessments showed relatively the same level of antiepileptogenic effects for KLFS and LFSK, quantitative assessments showed more significant differences in the quantitative measures between the two protocols. Developing more quantitative EEG based measures correlated with LFS-induced effects can facilitate developing open or closed loop seizure prevention modalities.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quantitative Analysis of the Antiepileptogenic Effects of Low Frequency Stimulation Applied Prior or After Kindling Stimulation in Rats

et al. 2018

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“…The power in the gamma band was reduced in parallel to increased power in the delta band. In a model of amygdala kindling, 34 increase in delta power and decrease in gamma and beta bands are coupled with epileptogenesis, seizures with behavioral components, and consciousness level. γ-aminobutyric acid A (GABA A ) receptors, ubiquitous in the central nervous system (CNS), might have a critical function in gamma frequency regulation.…”

Section: Discussionmentioning

confidence: 99%

Soman‐induced status epilepticus, epileptogenesis, and neuropathology in carboxylesterase knockout mice treated with midazolam

Marrero‐Rosado¹,

Furtado²,

Schultz³

et al. 2018

Epilepsia

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SummaryObjectiveExposure to chemical warfare nerve agents (CWNAs), such as soman (GD), can induce status epilepticus (SE) that becomes refractory to benzodiazepines when treatment is delayed, leading to increased risk of epileptogenesis, severe neuropathology, and long‐term behavioral and cognitive deficits. Rodent models, widely used to evaluate novel medical countermeasures (MCMs) against CWNA exposure, normally express plasma carboxylesterase, an enzyme involved in the metabolism of certain organophosphorus compounds. To better predict the efficacy of novel MCMs against CWNA exposure in human casualties, it is crucial to use appropriate animal models that mirror the human condition. We present a comprehensive characterization of the seizurogenic, epileptogenic, and neuropathologic effects of GD exposure with delayed anticonvulsant treatment in the plasma carboxylesterase knockout (ES1−/−) mouse.MethodsElectroencephalography (EEG) electrode‐implanted ES1−/− and wild‐type (C57BL/6) mice were exposed to various seizure‐inducing doses of GD, treated with atropine sulfate and the oxime HI‐6 at 1 minute after exposure, and administered midazolam at 15‐30 minutes following the onset of seizure activity. The latency of acute seizure onset and spontaneous recurrent seizures (SRS) was assessed, as were changes in EEG power spectra. At 2 weeks after GD exposure, neurodegeneration and neuroinflammation were assessed.Results GD‐exposed ES1−/− mice displayed a dose‐dependent response in seizure severity. Only ES1−/− mice exposed to the highest tested dose of GD developed SE, subchronic alterations in EEG power spectra, and SRS. Degree of neuronal cell loss and neuroinflammation were dose‐dependent; no significant neuropathology was observed in C57BL/6 mice or ES1−/− mice exposed to lower GD doses.SignificanceThe US Food and Drug Administration (FDA) animal rule requires the use of relevant animal models for the advancement of MCMs against CWNAs. We present evidence that argues for the use of the ES1−/− mouse model to screen anticonvulsant, antiepileptic, and/or neuroprotective drugs against GD‐induced toxicity, as well as to identify mechanisms of GD‐induced epileptogenesis.

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“…An increase in the power of the theta/delta bands or in the theta/alpha ratio during seizures has been reported in experimental animals or patients ( Ali et al. 2013 ; Faught et al., 1992 ; Goddard et al., 1969 ; Pinault et al., 2001 ; Monto et al., 2007 ; Motaghi et al., 2012 ; Jalilifar et al., 2016 ). On the other hand, Musto et al.…”

Section: Introductionmentioning

confidence: 90%

Delta-Frequency Augmentation and Synchronization in Seizure Discharges and Telencephalic Transmission

et al. 2020

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Summary Epileptic seizures constitute a common neurological disease primarily diagnosed by characteristic rhythms or waves in the local field potentials (LFPs) of cerebral cortices or electroencephalograms. With a basolateral amygdala (BLA) kindling model, we found that the dominant frequency of BLA oscillations is in the delta range (1–5 Hz) in both normal and seizure conditions. Multi-unit discharges are increased with higher seizure staging but remain phase-locked to the delta waves in LFPs. Also, the change in synchrony precedes and outlasts the changes in discharging units as well as behavioral seizures. One short train of stimuli readily drives the pyramidal-inhibitory neuronal networks in BLA slices into prolonged reverberating activities, where the burst and interburst intervals may concurrently set a “natural wavelength” for delta frequencies. Seizures thus could be viewed as erroneous temporospatial continuums to normal oscillations in a system with a built-in synchronizing and resonating nature for information relay.

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Classifying amygdala kindling stages using quantitative assessments of extracellular recording of EEG in rats

Cited by 14 publications

References 40 publications

Quantitative Analysis of the Antiepileptogenic Effects of Low Frequency Stimulation Applied Prior or After Kindling Stimulation in Rats

Quantitative Analysis of the Antiepileptogenic Effects of Low Frequency Stimulation Applied Prior or After Kindling Stimulation in Rats

Soman‐induced status epilepticus, epileptogenesis, and neuropathology in carboxylesterase knockout mice treated with midazolam

Delta-Frequency Augmentation and Synchronization in Seizure Discharges and Telencephalic Transmission

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