Classification, processing, and applications of bioink and 3D bioprinting: A detailed review

Raees, Sania; Javed, Fatima; Akil, Hazizan Md; Jadoon, Muhammad Zafar Iqbal; Safdar, Muhammad; Din, Israf Ud; Alotaibi, Mshari A.; Alharthi, Abdulrahman I.; Bakht, Md. Afroz; Ahmad, Akil; Nassar, Amal A.

doi:10.1016/j.ijbiomac.2023.123476

Cited by 47 publications

(58 citation statements)

References 231 publications

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“…Applying an electric charge to a polymer solution can produce high‐porosity and 3D structure nanofibers (Lee et al ., 2023; Peranidze et al ., 2023). 3D bioprinting allows for creating complex and physiologically relevant 3D structures (Raees et al ., 2023). Bioink refers to biomaterials that contain cells and are used for the 3D printing scaffolds and tissues (Choi et al ., 2016).…”

Section: Methods Of Fabricationmentioning

confidence: 99%

Review in edible materials for sustainable cultured meat: scaffolds and microcarriers production

Moslemy,

Sharifi,

Asadi‐Eydivand

et al. 2023

Int J of Food Sci Tech

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SummaryCultured meat, also known as lab‐grown or cell‐based meat, is a promising concept in the food industry that involves culturing animal cells in vitro to produce meat products, bypassing the need for traditional animal rearing and slaughter. This technology offers solutions to key challenges associated with conventional meat production, including environmental sustainability, animal welfare, and food security. However, the cultured meat industry must overcome significant hurdles to achieve commercial viability. Scaling up production to meet the increasing demand for meat is a considerable challenge, requiring a scalable edible scaffold, eliminating the need for separate scaffold removal, and ensuring product safety. Various edible materials, including polysaccharides and proteins, along with fabrication techniques like electrospinning and 3D printing, are explored in this review article.

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Section: Methods Of Fabricationmentioning

confidence: 99%

Review in edible materials for sustainable cultured meat: scaffolds and microcarriers production

Moslemy,

Sharifi,

Asadi‐Eydivand

et al. 2023

Int J of Food Sci Tech

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“…To generate a microgel-based bioresin, we first focused on the technology to produce light-sensitive particles. Although microgel slurries produced from virtually any light-responsive hydrogel could be used for EmVP, gelMA was selected as proof-ofconcept material for this study, due to its wide-spread use in bioprinting, [32] due to its biocompatibility and tunability for a large range of applications in both extrusion-based and lightbased bioprinting. [4,33,34] In this work, microgels were produced by mechanically breaking a thermally gelated, non-photoexposed, gelMA bulk hydrogel into microscale particles using a simple rotational blender, allowing to turn bulk hydrogels into microparticles within seconds or few minutes.…”

Section: μResins For Volumetric Bioprinting With Tunable Optical and ...mentioning

confidence: 99%

Shaping Synthetic Multicellular and Complex Multimaterial Tissues via Embedded Extrusion‐Volumetric Printing of Microgels

et al. 2023

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In living tissues, cells express their functions following complex signals from their surrounding microenvironment. Capturing both hierarchical architectures at the micro‐ and macroscale, and anisotropic cell patterning remains a major challenge in bioprinting, and a bottleneck toward creating physiologically‐relevant models. Addressing this limitation, a novel technique is introduced, termed Embedded Extrusion‐Volumetric Printing (EmVP), converging extrusion‐bioprinting and layer‐less, ultra‐fast volumetric bioprinting, allowing spatially pattern multiple inks/cell types. Light‐responsive microgels are developed for the first time as bioresins (µResins) for light‐based volumetric bioprinting, providing a microporous environment permissive for cell homing and self‐organization. Tuning the mechanical and optical properties of gelatin‐based microparticles enables their use as support bath for suspended extrusion printing, in which features containing high cell densities can be easily introduced. µResins can be sculpted within seconds with tomographic light projections into centimeter‐scale, granular hydrogel‐based, convoluted constructs. Interstitial microvoids enhanced differentiation of multiple stem/progenitor cells (vascular, mesenchymal, neural), otherwise not possible with conventional bulk hydrogels. As proof‐of‐concept, EmVP is applied to create complex synthetic biology‐inspired intercellular communication models, where adipocyte differentiation is regulated by optogenetic‐engineered pancreatic cells. Overall, EmVP offers new avenues for producing regenerative grafts with biological functionality, and for developing engineered living systems and (metabolic) disease models.

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“…To address these issues, three-dimensional (3D) bioprinting has attracted great interest for its ability to fabricate personalized scaffold structures based on predesigned models . This approach holds great promise in improving the survival, migration, and proliferation of the cell within the scaffolds by enhancing oxygen penetration, nutrient diffusion, and metabolomic waste exchange . In previous research, several natural or synthetic materials such as gelatin, collagen (Col), hyaluronic acid (HA), alginate (Alg), silk fibroin (SF), decellularized extracellular matrix (dECM), poly(ethylene glycol) (PEG), and Pluronic F127 (PF 127) have been introduced in 3D bioprinting …”

Section: Introductionmentioning

confidence: 99%

Customization of an Ultrafast Thiol–Norbornene Photo-Cross-Linkable Hyaluronic Acid–Gelatin Bioink for Extrusion-Based 3D Bioprinting

Xiao,

Yang,

Lai

et al. 2023

Biomacromolecules

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Light-based three-dimensional (3D) bioprinting has been widely studied in tissue engineering. Despite the fact that freeradical chain polymerization-based bioinks like hyaluronic acid methacrylate (HAMA) and gelatin methacryloyl (GelMA) have been extensively explored in 3D bioprinting, the thiol−ene hydrogel system has attracted increasing attention for its ability in building hydrogel scaffolds in an oxygen-tolerant and cell-friendly way. Herein, we report a superfast curing thiol−ene bioink composed of norbornene-modified hyaluronic acid (NorHA) and thiolated gelatin (GelSH) for 3D bioprinting. A new facile approach was first introduced in the synthesis of NorHA, which circumvented the cumbersome steps involved in previous works. Additionally, after mixing NorHA with macro-cross-linker GelSH, the customized NorHA/GelSH bioinks exhibited fascinating superiorities over the gold standard GelMA bioinks, such as an ultrafast curing rate (1−5 s), much lowered photoinitiator concentration (0.03% w/v), and flexible physical performances. Moreover, the NorHA/ GelSH hydrogel greatly avoided excess ROS generation, which is important for the survival of the encapsulated cells. Last, compared with the GelMA scaffold, the 3D-printed NorHA/GelSH scaffold not only exhibited excellent cell viability but also guaranteed cell proliferation, revealing its superior bioactivity. In conclusion, the NorHA/GelSH system is a promising candidate for 3D bioprinting and tissue engineering applications.

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Classification, processing, and applications of bioink and 3D bioprinting: A detailed review

Cited by 47 publications

References 231 publications

Review in edible materials for sustainable cultured meat: scaffolds and microcarriers production

Review in edible materials for sustainable cultured meat: scaffolds and microcarriers production

Shaping Synthetic Multicellular and Complex Multimaterial Tissues via Embedded Extrusion‐Volumetric Printing of Microgels

Customization of an Ultrafast Thiol–Norbornene Photo-Cross-Linkable Hyaluronic Acid–Gelatin Bioink for Extrusion-Based 3D Bioprinting

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