Classification of strains of Xanthomonas oryzae on the basis of their susceptibility against bacteriophages

Wakimoto, Satoshi

doi:10.3186/jjphytopath.25.193

Cited by 25 publications

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“…Wakimoto (1960) classified Xoo into five "lysotypes" according to the infectivity of four phages. However, in recent years, "phagovar" has been used to group the bacteria based on infection type of phages (Yoshida 2001).…”

Section: Methodsmentioning

confidence: 99%

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Bacteriophage OP1, lytic for Xanthomonas oryzae pv. oryzae, changes its host range by duplication and deletion of the small domain in the deduced tail fiber gene

Inoue

Matsuura

Ohara³

et al. 2006

J Gen Plant Pathol

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Section: Methodsmentioning

confidence: 99%

“…In a study of host specificity between h-mutants of OP 1 and bacterial strains, Xoo strains from Japan were classified into four groups on the basis of their susceptibility (Wakimoto 1960). These data are useful for the analysis of the host specificity of phages.…”

Section: Introductionmentioning

confidence: 99%

Bacteriophage OP1, lytic for Xanthomonas oryzae pv. oryzae, changes its host range by duplication and deletion of the small domain in the deduced tail fiber gene

Inoue

Matsuura

Ohara³

et al. 2006

J Gen Plant Pathol

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“…OP 2 is a phage of Xanthomonas oryzae pv. oryzae that differs from OP 1 -related phages (Wakimoto 1960). Xoo1687::KAN1(pHM1) and Xoo1687::KAN1(pHMIroNB) are the transformants of Xoo1687::KAN1 with pHM1 and pHMIroNB, respectively phosphotungstic acid (w/v) for 10 min.…”

Section: Electron Microscopic Analysismentioning

confidence: 99%

“…Generation and isolation of OP 1h2 -tolerant mutants from Xoo strain T7174R by transposon mutagenesis Xoo phage OP 1 , OP 1h and OP 1h2 are closely related to each other in their genetic, serological and morphological features, although their host specificity differs (Wakimoto 1960;Inoue et al 2006a). Xoo phage OP 2 , however, differs genetically, serologically and morphologically from OP 1 (Yoshimura et al 1960;Inoue et al 2006b).…”

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Adsorption of OP1-related bacteriophages requires the gene encoding a TonB-dependent receptor-like protein in Xanthomonas oryzae pv. oryzae

et al. 2007

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Xanthomonas oryzae pv. oryzae strain T7174R is lysed by bacteriophage OP 1h and OP 1h2 . Three mutants tolerant to both OP 1h and OP 1h2 were isolated by transposon mutagenesis. The mutants had an insertion of the transposon in XOO1687, which is predicted to encode a TonB-dependent receptor gene. Plasmid pHMIroNB that contained XOO1687 of T7174R was constructed, and the mutant was transformed with the plasmid. The transformant recovered sensitivity to OP 1h and OP 1h2 . Electron microscopic analysis demonstrated that OP 1h and OP 1h2 can adsorb to the wild type and the transformant, but they could not adsorb to the phage-tolerant mutant. These results suggest that the TonB-dependent receptor gene relates to adsorption and infection of T7174R by OP 1h2 and OP 1h .

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Detection of Bacterial and Phytoplasmal Pathogens

Narayanasamy¹

2010

Microbial Plant Pathogens-Detection and Disease Diagnosis:

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Bacterial pathogens classified as prokaryotes are much simpler in structure and smaller in size and they have less complex structural features compared to fungal plant pathogens. As the morphological characteristics of bacterial cells are less variable, biological, biochemical, physiological, immunological and genomic characteristics have to be determined for reliable identification and meaningful classification of bacterial pathogens. Detection and identification of bacterial plant pathogens present in whole plants and in propagative plant materials have been possible by employing isolation on cultural media and metabolic fingerprinting methods. But they are labor-intensive and require long time. Results often are inconclusive. Isozyme analysis, direct colony thin layer chromatography and gel electrophoresis techniques have been successfully applied for the detection of some bacterial pathogens. Immunoassays and nucleic acid-based assays have become widely accepted techniques, providing more sensitive and specific detection and quantification of bacterial pathogens affecting a wide range of host plant species. However, the major limitation of the molecular techniques is their inability to discriminate living cells from the dead ones. Attempts have been made to overcome this limiting factor by using DNA binding dyes and estimating pathogen RNAs as an indicator of cell viability. The diagnostic methods have both merits and demerits and hence, appropriate method has to be selected based on cost-effectiveness and possibility of obtaining reliable results rapidly to suit the requirements of the investigation concerned.Phytoplasmas are cell wall-less, nonculturable bacteria belonging to Mollicutes. Lack of cultural characteristics has made obligatory to study the morphological characteristics of phytoplasmas present in the phloem cells of infected plant hosts by observing under electron microscopes. As most of the phytoplasmas look alike in the ultrathin sections, the morphological characters have no diagnostic value. Histochemical methods using DNA binding dyes have been employed to localize the phytoplasmas in the host cells. Application of immunoassays and nucleic acidbased techniques has been effective in providing information for the identification and differentiation of phytoplasams. Polyclonal and monoclonal antibodies have been generated for detection of phytoplasmas infecting several plant species. Universal and species-specific primers and probes have been designed based on

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Classification of strains of Xanthomonas oryzae on the basis of their susceptibility against bacteriophages

Cited by 25 publications

References 0 publications

Bacteriophage OP1, lytic for Xanthomonas oryzae pv. oryzae, changes its host range by duplication and deletion of the small domain in the deduced tail fiber gene

Bacteriophage OP1, lytic for Xanthomonas oryzae pv. oryzae, changes its host range by duplication and deletion of the small domain in the deduced tail fiber gene

Adsorption of OP1-related bacteriophages requires the gene encoding a TonB-dependent receptor-like protein in Xanthomonas oryzae pv. oryzae

Detection of Bacterial and Phytoplasmal Pathogens

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