Classification of stevia sweeteners in soft drinks using liquid chromatography and time-of-flight mass spectrometry

Abstract

The aim of this study was to develop a comprehensive analytical method for the characterisation of stevia sweeteners in soft drinks. By using LC and time-of-flight MS, we detected 30 steviol glycosides from nine stevia sweeteners. The mass spectral data of these compounds were applied to the analysis to determine steviol glycosides in nine soft drinks. On the basis of chromatographic data and principal-component analysis, these soft drinks were classified into three groups, and the soft drinks of each group, respectively, contained high-rebaudioside A extract, normal stevia extract or alfa-glucosyltransferase-treated stevia extract.

An ultrasensitive performance enhanced novel cytochrome c biosensor for the detection of rebaudioside A

“…A literature survey of the analytical instrumentation used in the food industry revealed that high performance liquid chromatography (Woelwer-Rieck et al, 2010;Zimmermann et al, 2011;Puri et al, 2012), high performance thin layer chromatography (Jaitak et al, 2008;Londhe and Nanaware, 2013), hydrophilic interaction liquid chromatography (Well et al, 2013), liquid chromatographymass spectrometry (Shah et al, 2012;Kakigi et al, 2013), liquid chromatography coupled to electro spray ionization mass spectrometry (Rajasekaran et al, 2008;Montoro et al, 2013) and capillary electrophoretic methods (Bathinapatla et al, 2015a) have been employed for the determination of steviol glycosides, mainly Reb A in different food samples. On the other hand electrochemical methods, in contrast have significant advantages such as faster response time, lower cost, higher sensitivity and selectivity.…”

An in-silico layer-by-layer adsorption study of the interaction between Rebaudioside A and the T1R2 human sweet taste receptor: modelling and biosensing perspectives

“…Literature studies revealed that diverse analytical methods have been employed in the food industry to quantify steviol glycosides, such as Reb-A. Some of these instruments include liquid chromatography-mass spectrometry 19 , 20 , high performance liquid chromatography (HPLC) 21 , 22 , high performance thin layer chromatography 23 , and capillary electrophoretic methods 24 , 25 , among others 26 . In contrast, noteworthy advantages such as low cost, higher sensitivity and selectivity with quick response times, are attributed to electrochemical methods.…”

Chemistry and analytical techniques for ent-kaurene-glycosides of Stevia rebaudiana Bertoni - A review

“…Stevioside and rebaudioside A−C have been detected by 1H NMR spectroscopy (Inamake et al, 2010). Furthermore, reference compounds are not used for identification of desired compounds in NMR spectroscopy as compared to HPLC and LC-UV method (Kakigi et al, 2013).…”

“…Shah et al (2012) developed a LC-MS/MS method for the characterization of steviol glycosides in foods. Kakigi et al (2013) developed an analytical method for characterizing stevia sweeteners in soft drinks by using LC and MS methods. Kubica et al (2015) reported a method (high-performance liquid chromatography and tandem mass spectroscopy with electrospray ionisation (HPLC-ESI-MS/MS) for the determination of synthetic sweeteners (acesulfame-K, aspartame, alitame, cyclamate, saccharine, sucralose, neohesperidin dihydrochalcone, neotame) and steviol glycosides (steviol, steviolbioside, rebaudioside A, rebaudioside C and stevioside) in beverages.…”