Classification of staphylococci based on chemical and biochemical properties

Schleifer, Karl Heinz; Kocur, M.

doi:10.1007/bf00666081

Cited by 67 publications

(33 citation statements)

References 45 publications

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“…Peptococcus saccharolyticus falls into the Staphylococcus cluster Table 3. It should be noted that Staphylococcus epidermidis ATCC 155 does not fit the description for S. epidermidis sensu stricto (Schleifer & Kocur, 1973;Schleifer & Kloos, 1975): it differs in several biochemical characters from a typical S. epidermidis and more closely resembles S. warneri. However, DNA-DNA reassociation studies established that S. epidermidis .and S. warneri, together with S. capitis, form a closely related species group (Schleifer et al, 1979a).…”

Section: R E S U L T Smentioning

confidence: 93%

A Phylogenetic Analysis of Staphylococci, Peptococcus saccharolyticus and Micrococcus mucilaginosus

Ludwig¹,

Schleifer²,

Fox

et al. 1981

Microbiology

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The intra-and intergeneric relationships of the genus Staphylococcus, and the phylogenetic position of Peptococcus saccharolyticus and Micrococcus mucilaginosus (Staphylococcus salivarius), were investigated by comparative oligonucleotide cataloguing of 16s rRNA. All the staphylococci investigated form a phylogenetically coherent group at the genus level that, in addition, contains the anaerobic species Peptococcus saccharolyticus. The genus Staphylococcus belongs to the broad Bacillus-Lactobacillus-Streptococcus cluster that is defined by Gram-positive bacteria with a low DNA G + C content.Micrococcus mucilaginosus is not a genuine member of the genus Micrococcus. The binary matching coefficients between the 16s rRNA of Micrococcus mucilaginosus and those of representatives of the A rthrobacterlMicrococcus group and related genera indicate that Micrococcus mucilaginosus should be regarded as a member of a new genus.

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Section: R E S U L T Smentioning

confidence: 93%

A Phylogenetic Analysis of Staphylococci, Peptococcus saccharolyticus and Micrococcus mucilaginosus

Ludwig¹,

Schleifer²,

Fox

et al. 1981

Microbiology

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“…Thirteen reference strains were used: Staphylococcus caprae (DSM 20608) (Kawamura et al, 1998); Staphylococcus cohnii (DSM 20260), Staphylococcus haemolyticus (DSM 20263), Staphylococcus saprophyticus (DSM 20229) and Staphylococcus xylosus (DSM 20267) ; Staphylococcus hominis (DSM 20328), Staphylococcus simulans (DSM 20322) and Staphylococcus warneri (DSM 20316) ; Staphylococcus epidermidis (DSM 1798) (Winslow, 1908); Staphylococcus hyicus (DSM 20459) (Schleifer & Kocur, 1973); Staphylococcus intermedius (DSM 20373) (Hajek, 1976); and Staphylococcus lugdunensis (DSM 4804) and Staphylococcus schleiferi (DSM 4807) (Freney et al, 1988).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of species-specific score cut-off values for various Staphylococcus species using a MALDI Biotyper-based identification

Richter

Hollstein

Woloszyn

et al. 2012

Journal of Medical Microbiology

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Although previous studies investigating the MALDI Biotyper database (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry-based identification) have proven its high accuracy for bacterial identification, the studies differed in sample preparation, number of replicates, quantity of shots and target types used. In particular, the score cut-off values of special importance for reliable species identification varied. The aim of the present study was to identify species-specific differences in the mean score values for staphylococci. Cut-off values recommended by the manufacturer were adapted using the 20th percentile to rule out unknown score-modifying factors, even though the specificity was high and the lowest cut-off values would also yield an accurate result. Whilst correct species diagnosis was obtained in 97.32 % of samples (1382/1420), only 220 of all duplicates (15.49 %) revealed a score of ¢2.3, whilst 968 (68.17 %) had a score between 2.0 and 2.299, and 194 (13.66 %) had a score of ,2.0. Ten of 21 species had a calculated 20th percentile of ,2.0 and one species of ,1.7. In conclusion, the use of species-specific cut-off values improves the relative sensitivity of species identification in staphylococci.

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“…The compositions of cell wall teichoic acids were determined as previously described (27). The procedure used for determining the configurations of the lactic acids produced has also been described previously (28,29). The guanine-plus-cytosine contents of deoxyribonucleic acids (DNAs) were determined by the thermal denaturation method of Marmur and Doty (23).…”

Section: Methodsmentioning

confidence: 99%

Staphylococcus gallinarum and Staphylococcus caprae, Two New Species from Animals

Devriese

Poutrel

Kilpper-Bälz

et al. 1983

International Journal of Systematic Bacteriology

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103

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We describe a new species, Staphylococcus gallinarum, based on a study of characteristics of strains isolated from chickens and a pheasant. Strain CCM 3572 (= VIII1) is the type strain of this species. The chemical composition of the cell walls of the strains of this new species is similar to that of Staphylococcus epidermidis, but these organisms are resistant to novobiocin and produce acid from a wide range of carbohydrates. Staphylococcal strains isolated from goat milk belong to another new species, Staphylococcus caprae. The type strain is CCM 3573 (= 143.22). These strains are more closely related to S. epidermidis and Staphylococcus capitis than to other staphylococci.

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Classification of staphylococci based on chemical and biochemical properties

Cited by 67 publications

References 45 publications

A Phylogenetic Analysis of Staphylococci, Peptococcus saccharolyticus and Micrococcus mucilaginosus

A Phylogenetic Analysis of Staphylococci, Peptococcus saccharolyticus and Micrococcus mucilaginosus

Evaluation of species-specific score cut-off values for various Staphylococcus species using a MALDI Biotyper-based identification

Staphylococcus gallinarum and Staphylococcus caprae, Two New Species from Animals

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