Let (M, ω M ) be a six dimensional closed monotone symplectic manifold admitting an effective semifree Hamiltonian S 1 -action. We show that (M, ω M ) is S 1 -equivariant symplectomorphic to some Kähler Fano manifold (X, ω X , J) with a certain holomorphic C * -action. We also give a complete list of all such Fano manifolds and describe all semifree C * -actions on them specifically.