Classification of Nonomuraea sp. ATCC 39727, an actinomycete that produces the glycopeptide antibiotic A40926, as Nonomuraea gerenzanensis sp. nov.

A valuable peroxidase activity from the novel species Nonomuraea gerenzanensis growing on alkali lignin

“…Although more active at acidic than at basic pHs, N. gerenzanensis peroxidase activity is more stable at higher pHs than the fungal counterparts. This finding is coherent with the ecological niche from which this actinomycete was isolated: it was demonstrated that it grows easily at pHs of 10.0 and 11.0 [31], whereas fungi usually prefer acidic environments. From a kinetic point of view, N. gerenzanensis peroxidase activity shows a K m for H 2 O 2 similar to that of P. chrysosporium and Bjerkandera MnPs (≈ 30–55 μM) and a lower affinity for Mn 2+ ( K m  ≈ 50–80 μM) [46], [49].…”

“…The genome of S. coelicolor A3(2) contains a gene for a two-domain laccase, called SLAC [25], [38], [39], whose role in degrading lignocellulosic biomass was recently demonstrated [16]. N. gerenzanensis is a recently classified species [31] and its genome is not yet available; to our knowledge, this is the first report on its ability to use soluble lignin derivatives for growing.…”

“…Nonomuraea gerenzanensis [31] and Streptomyces coelicolor A3(2) working cell banks (WCBs) were prepared as previously described [29]. Preinoculum cultures were set up by inoculating 0.75 ml of the WCB into 15 ml VM liquid medium (in g/l: 24 soluble starch, 1 glucose, 3 meat extract, 5 yeast extract, and 5 tryptose) in 100 ml Erlenmeyer flasks, incubated at 28 °C and 200 revolutions per minute (rpm) for 72 h. An aliquot of 1.8 ml of these cultures was transferred into 300-ml baffled Erlenmeyer flasks containing 50 ml of two different basal media, VM and MM-L (liquid version, without agar), to which the following components could be added: 0.8 or 1.5 g/l alkali lignin, 6 or 12 g/l yeast extract, 2 mM MnCl 2 , 2 mM CuSO 4 , 0.2 mM FeSO 4 ·7H 2 O, 5 mM tryptophan, 0.5 g/l mannose, 0.5 g/l glucose, 6 g/l meat extract, 1 g/l hydrolyzed casein, and 3.5 or 5.0% v/v ethanol.…”

“…Following this approach, we discovered and investigated the biochemical properties of a novel and efficient peroxidase activity produced by a Nonomuraea strain ( Streptosporangiaceae family) recently classified as Nonomuraea gerenzanensis [31] that might be involved in the natural metabolism of lignin.…”

The complete 12 Mb genome and transcriptome of Nonomuraea gerenzanensis with new insights into its duplicated “magic” RNA polymerase

“…Here we present the complete genome sequence of Nonomuraea gerenzanensis ATCC 39727, an industrially important microorganism that was isolated from Indian soil 4 . This microorganism is the producer of the teicoplanin-like glycopeptide A40926 with anti -Neisseria activity 5 , which is the precursor of dalbavancin, a novel second-generation lipoglycopeptide antimicrobial with unique pharmacokinetics and excellent activity against resistant gram-positive pathogens, including methicillin-resistant Staphylococcus aureus 6 .…”