Classification of Mycobacterium farcinogenes and Mycobacterium senegalense by Immunodiffusion and Thin-layer Chromatography of Long-chain Components

Ridell, Malin; Goodfellow, Michael; Minnikin, David E.; Minnikin, S. M.; Hutchinson, I

doi:10.1099/00221287-128-6-1299

Cited by 18 publications

(24 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…farciizogenes and M . senegalense confirms earlier work which used lipid and immunological analyses (Ridell et al, 1982). In addition, the mycobactin produced by N .…”

Section: R E S U L T S a N D Discussionsupporting

confidence: 76%

See 1 more Smart Citation

Equivalence of Mycobactins from Mycobacterium senegalense, Mycobacterium farcinogenes and Mycobacterium fortuitum

Hall

Ratledge

1985

Microbiology

View full text Add to dashboard Cite

1691Mycobactins were isolated from five strains designated Mycobacterium jarcinogenes and a similar number designated Mycobacterium senegalense following growth under conditions of iron-limitation. These lipid-soluble iron-chelating compounds were characterized by a combination of thin-layer and high-performance liquid chromatography. The mycobactins from both the slow-growing M . jbrcinogenes and the rapidly-growing M . senegalense strains proved impossible to differentiate both from each other and from those produced by strains of MJicobacterium jurtuitum, indicating a close relationship between all three species. However, Nocardia jbrcinica, previously implicated with the bovine farcy strains, produced a different mycobactin which was easily distinguished by thin-layer chromatography alone.

show abstract

“…farciizogenes and M . senegalense confirms earlier work which used lipid and immunological analyses (Ridell et al, 1982). In addition, the mycobactin produced by N .…”

Section: R E S U L T S a N D Discussionsupporting

confidence: 76%

“…On the basis of lipid analysis (Ridell et al, 1982), M.ji2rcinogenes and M . senegalense could not be differentiated ; each contained a-mycolates and characteristic polar mycolates, derived from epoxymycolic acids, a pattern also found in M .…”

Section: R E S U L T S a N D Discussionmentioning

confidence: 99%

Equivalence of Mycobactins from Mycobacterium senegalense, Mycobacterium farcinogenes and Mycobacterium fortuitum

Hall

Ratledge

1985

Microbiology

View full text Add to dashboard Cite

show abstract

“…Species containing relatively long-chain mycolic acids (48 to 66 carbon atoms) and MK-9 (H2) as the predominant menaquinone type, were found to be homogeneous with respect to their 16S ribosomal RNA sequences. Beside chemotaxonomic methods, numerical taxonomic studies have been found to be very important in the taxonomy of these genera (6,7,11,20,(28)(29)(30)(31)42,45).…”

mentioning

confidence: 99%

Numerical classification and identification of some nocardioform bacteria.

Kämpfer

Dott

Kroppenstedt

1990

J. Gen. Appl. Microbiol.

View full text Add to dashboard Cite

A total of 254 strains of the genera Nocardia, Rhodococcus, Amycolata, Amycolatopsis, Gordona and Pseudoamycolata were compared using numerical taxonomic techniques based on 273 physiological characters with the aid of miniaturized tests. Clustering was achieved using the unweighted pair group method with arithmetic averages (UPGMA) and the simple-matching coefficient (SSM) as the measure for similarity. Test error and overlap between the phena were within acceptable limits. Cluster groups were defined at the 87.4 to 91.3 % levels (SSM). A total of 25 clusters were obtained in the SSM/UPGMA analysis beside 16 single member clusters, which in most cases were marker strains of different species. For the 25 clusters, containing two or more strains, a matrix comprising frequencies for positive results of 35 tests was constructed. The minimum number of diagnostic characters was selected by computer programs (CHARSEP, DIACHAR, MOSTTYP). The final matrix consisting of 35 tests versus 25 phena was theoretically evaluated using a computer program (MATIDEN) and out of 238 strains, a total of 157 strains (65.96%) were correctly identified with a Willcox probability >0.999, further 18 strains (7.56%) achieved Willcox probabilities above 0.900 and 2 strains (0.84%) were identified correctly with a Willcox probability above 0.800. Correct identification results were obtained for a total of 56 strains (23.52%), but with low Willcox probabilities ranging between 0.500 and 0.800. A total of 5 strains (2.10%) could not be assigned to the correct phenon. In a subsequent practical evaluation of the matrix 32 of 40 tested strains (80.0%) were correctly identified with Willcox probabilities above 0.900.The genera Rhodococcus and Nocardia belong to the actinomycetes which are now largely defined on the basis of cell envelope lipids and peptidoglycan composition (8,40). The cell walls of representative nocardiae and rhodococci * Address reprint requests to: Dr . P. Kampfer, Fachgebiet Hygiene,

show abstract

“…Mycolic acids, which are 2-alkyl, 3-hydroxy longchain fatty acids, have particular importance in the classification of actinomycetes with a wall chemotype IV based on meso-diaminopimelic acid, arabinose and galactose (Lechevalier et al, 1971;Ridell et al, 1982;Minnikin et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

“…There is evidence, however, that strains of these mycobacterial species have frequently been mis-identified as N . farcinica (Ridell & Goodfellow, 1983;Ridell et al, 1982Ridell et al, , 1985 and reliable methods are needed to distinguish M . farcinogenes from N .…”

Section: Introductionmentioning

confidence: 99%