Classification of mouse B cell types using surfaceome proteotype maps

Oostrum, Marc van; Müller, Marion; Klein, Fabian; Bruderer, Roland; Zhang, Hui; Pedrioli, Patrick G. A.; Reiter, Lukas; Tsapogas, Panagiotis; Rolink, Antonius; Wollscheid, Bernd

doi:10.1038/s41467-019-13418-5

Cited by 36 publications

(52 citation statements)

References 22 publications

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“…Recently, we reported a miniaturization and automation of the Cell Surface Capture (autoCSC) method, enabling sensitive and multiplexed interrogation of the surfaceome landscape by direct identification of extracellular N-glycopeptides 30,31 . On living cells, cell-surface carbohydrates are tagged with cell-impermeable biocytin-hydrazide.…”

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confidence: 99%

Surfaceome dynamics reveal proteostasis-independent reorganization of neuronal surface proteins during development and synaptic plasticity

et al. 2020

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Neurons are highly compartmentalized cells with tightly controlled subcellular protein organization. While brain transcriptome, connectome and global proteome maps are being generated, system-wide analysis of temporal protein dynamics at the subcellular level are currently lacking. Here, we perform a temporally-resolved surfaceome analysis of primary neuron cultures and reveal dynamic surface protein clusters that reflect the functional requirements during distinct stages of neuronal development. Direct comparison of surface and total protein pools during development and homeostatic synaptic scaling demonstrates system-wide proteostasis-independent remodeling of the neuronal surface, illustrating widespread regulation on the level of surface trafficking. Finally, quantitative analysis of the neuronal surface during chemical long-term potentiation (cLTP) reveals fast externalization of diverse classes of surface proteins beyond the AMPA receptor, providing avenues to investigate the requirement of exocytosis for LTP. Our resource (neurosurfaceome.ethz.ch) highlights the importance of subcellular resolution for systems-level understanding of cellular processes.

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confidence: 99%

Surfaceome dynamics reveal proteostasis-independent reorganization of neuronal surface proteins during development and synaptic plasticity

et al. 2020

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“…To obtain a more global picture of the alteration of protein expression on the surface of CD20-deficient Ramos B cells we took advantage of the Cell Surface Capture (CSC) technology 14,15 . CSC technology allows for the efficient labelling, identification and relative quantification of N-glycosylated proteins residing on the cell surface and provides a snapshot of the surfaceome without having to use antibodies to broadly phenotype the B cells 16 .…”

Section: Loss Of Cd20 Expression Is Associated With B Cell Activationmentioning

confidence: 99%

CD20 as a gatekeeper of the resting stage of human B cells

Kläsener

Jellusova

Andrieux

et al. 2020

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CD20 is a B cell specific membrane protein and a target of therapeutic antibodies such as rituximab (RTX). In spite of the prominent usage of anti-CD20 antibodies in the clinic little is known about the biological function of CD202. Here we show that CD20 controls the nanoscale organization of receptors on the surface of resting B lymphocytes. A CRISPR/Cas-based ablation of CD20 in Ramos B cells results in a relocalisation of the IgM B cell antigen receptor (IgM-BCR) and the co-receptor CD19. The resulting IgM-BCR/CD19 signaling synapse leads to transient B cell activation followed by plasma cell differentiation. Similarly to CD20-deficient Ramos cells, naive human B cells treated with rituximab in vitro or isolated from patients during rituximab administration display hallmarks of transient activation characterized by the formation of the IgM-BCR/CD19 signaling synapse, followed by CD19 and IgM-BCR downregulation. Moreover, increased expression of specific plasma cell genes can be observed after rituximab treatment in relapsed CLL patients. In summary we identify CD20 as a gatekeeper of the resting state on human B cells and demonstrate that a disruption of the nanoscale organization of the B cell surface via CD20 deletion or anti-CD20 treatment profoundly alters B cell fate.

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“…Dedicated strategies have been established to profile cellular surfaceomes 7 . For example, the extensive application of the Cell Surface Capture (CSC) technology 8,9 established N-glycosylated surfaceomes for numerous cell types (collectively reported in the Cell Surface Protein Atlas, CSPA https://wlab.ethz.ch/cspa/) 10 and enabled the in silico characterization of the entire human surfaceome 11 . While, fluorescence-based reporter assays [12][13][14] and platforms of genetically engineered receptors [15][16][17] established associations between receptors by high-throughput testing of binary interactions within and across surfaceomes, the Ligand-Receptor Capture (LRC) technology identified direct ligand interactions of N-glycosylated receptors 18,19 .…”

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confidence: 99%

Light-mediated discovery of surfaceome nanoscale organization and intercellular receptor interaction networks

Müller

Gräbnitz

Barandun

et al. 2020

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Delineating the molecular nanoscale organization of the surfaceome is pre-requisite for understanding cellular signaling. Technologies for mapping the spatial relationships of cell surface receptors and their extracellular signaling synapses would open up theranostic opportunities and the possibility to engineer extracellular signaling. Here, we developed an optoproteomic technology termed LUX-MS that exploits singlet oxygen generators (SOG) for the light-triggered identification of acute protein interactions on living cells. Using SOG-coupled antibodies, small molecule-drugs, biologics and intact viral particles, we show that not only ligand-receptor interactions can be decoded across organisms, but also the surfaceome receptor nanoscale organization ligands engage in with direct implications for drug action. Furthermore, investigation of functional immunosynapses revealed that intercellular signaling inbetween APCs and CD8+ T cells can be mapped now providing insights into T cell activation with spatiotemporal resolution. LUX-MS based decoding of surfaceome signaling architectures provides unprecedented molecular insights for the rational development of theranostic strategies.

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Classification of mouse B cell types using surfaceome proteotype maps

Cited by 36 publications

References 22 publications

Surfaceome dynamics reveal proteostasis-independent reorganization of neuronal surface proteins during development and synaptic plasticity

Surfaceome dynamics reveal proteostasis-independent reorganization of neuronal surface proteins during development and synaptic plasticity

CD20 as a gatekeeper of the resting stage of human B cells

Light-mediated discovery of surfaceome nanoscale organization and intercellular receptor interaction networks

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