Classification of hepatitis B virus genotypes by the PCR-Invader method with genotype-specific probes

Tadokoro, Kenichi; Kobayashi, Mariko; Yamaguchi, Toshikazu; Suzuki, Fumitaka; Miyauchi, Saeko; Egashira, Toru; Kumada, Hiromitsu

doi:10.1016/j.jviromet.2006.07.014

Cited by 43 publications

(48 citation statements)

References 20 publications

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“…When the FRET is cleaved, a fluorophore is released from a quencher on the FRET and generates a fluorescence signal. 16,17 Ten mutations of exon18 (G719A, G719C and G719S), exon19 {E746-A750del (nt 2235-2249del), E746-A750del (nt 2236-2250del) and L747-P753del ins S}, exon20 (S768I and T790M) and exon21 (L858R and L861Q) were determined. The PCR invader method was performed by BML (Tokyo, Japan).…”

Section: Pcr Invader Methodsmentioning

confidence: 99%

“…Previous authors have reported that the PCR invader method was useful for detecting polymorphisms and identifying genotypes. 16,17 These three methods are known to be highly sensitive: their sensitivities are approximately the same. 18 These PCR methods are in common clinical use; however, they have not been directly and prospectively compared with respect to clinical sensitivity and specificity.…”

Section: Introductionmentioning

confidence: 99%

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Direct Comparison of 3 PCR Methods in Detecting EGFR Mutations in Patients with Advanced Non–Small-Cell Lung Cancer

Ikeda

Nakamura

Yamaguchi

et al. 2012

Clinical Lung Cancer

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Micro abstractWe compared three PCR methods (mutant-enriched PCR, PNA-LNA PCR and PCR clamp) to detect EGFR mutations in 50 patients with advanced NSCLC. Seventeen were harboring EGFR mutations, five of whom showed discrepancies between the results of different PCR methods. All five responded to gefitinib, which we consider to suggest that the discrepancies were false negatives. Clinical Practice Points• Several methods have been used to detect EGFR mutations in non-small-cell lung cancer (NSCLC): however, it is not clear which is the most suitable for use in the clinic.• We compared three PCR methods (mutant-enriched PCR, PNA-LNA PCR and PCR clamp) in 50 patients with advanced NSCLC. Seventeen of the patients were harboring EGFR mutations, five of whom showed discrepancies between the results of different PCR methods. All five patients responded to gefitinib.• We considered that all of the discrepancies might be false negatives because the patients responded to gefitinib. To clarify the reasons for the false negatives of each PCR method, and establish the clinical sensitivity and specificity of each method, a large prospective clinical trial is warranted. AbstractBackground: Epidermal growth factor receptor (EGFR) mutations are predictive of

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Section: Pcr Invader Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Direct Comparison of 3 PCR Methods in Detecting EGFR Mutations in Patients with Advanced Non–Small-Cell Lung Cancer

Ikeda

Nakamura

Yamaguchi

et al. 2012

Clinical Lung Cancer

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“…Hepatitis B e antigens (HBeAg) and antiHBe were assayed with CLIA, and the HBV-DNA levels were assayed using a real-time polymerase chain reaction (PCR) method. The HBV genotypes were determined using a serial invasive signal amplification reaction assay (Invader assay; BML Inc, Saitama, Japan) (13). When genotype could not be identified with this method, it was also assayed using an enzyme immunoassay (EIA) method (Immunis , HBV genotype EIA; Institute of Immunology Co., Ltd, Tokyo, Japan) (14).…”

Section: Methodsmentioning

confidence: 99%

Clinical Features and Hepatitis B Virus (HBV) Genotypes in Pregnant Women Chronically Infected with HBV

et al. 2012

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Objective The purpose of this study was to clarify the clinical features and hepatitis B virus (HBV) genotypes in pregnant women chronically infected with HBV. Methods Among 1,489 pregnant women who visited our hospital in 2010, 26 were positive for hepatitis B surface antigens (HBsAg). Of these subjects, 21 from whom informed consent was obtained were included in this study. The clinical features and HBV markers, including genotypes, were investigated. Results No adverse events were observed in the subjects or the neonates during pregnancy or the perinatal period. The HBV genotypes were C in 14 cases, D in six cases, and undetermined in one case. Hepatitis B e antigens and a high viral load (>7.0 log copies/mL) were found in four and six subjects with genotype C, respectively, and in none of subjects with genotype D. The alanine aminotransferase (ALT) levels and platelet counts were within the normal ranges during pregnancy in all subjects except two and three subjects with genotype C, respectively. Three subjects with genotype C showed transient elevations of ALT after delivery. Conclusion The majority of subjects were anti-HBe-positive with normal ALT levels; however, some subjects with genotype C showed a high viral load, elevated ALT levels and/or low platelet counts. The pregnancies and deliveries were safe; however, transient elevations of ALT after delivery were observed in some subjects with genotype C.

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“…The titer of HBV DNA was high at 7.5 log copies/ml. The HBV genotype was Bj in Sugauchi et al's classification [13], which was determined by the PCR invader method [14]. The precore mutant HBV was 60%, whereas the wild-type HBV was 40% on the basis of the PCR enzyme-linked minisequence assay.…”

Section: Case Reportmentioning

confidence: 99%

A case of lamivudine-sensitive de novo acute hepatitis B induced by rituximab with the CHOP regimen for diffuse large B cell lymphoma

et al. 2008

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A case of de novo acute hepatitis B that showed symptoms of general malaise and anorexia during rituximab therapy with the CHOP regimen for diffuse large B cell lymphoma is reported. Lamivudine was strikingly effective, showing a rapid recovery from liver damage with jaundice. Hepatitis B virus (HBV) DNA in serum became and stayed undetectable even after the withdrawal of lamivudine, although HBsAg remained positive over 42 months from the onset. Liver biopsy showed a picture suggestive of acute viral hepatitis with multinucleated giant hepatocytes and CD38-positive plasma cell infiltration into liver parenchyma. Immunohistochemically, CD3-positive T-cells were predominant cells that infiltrated in liver parenchyma, whereas CD20-positive B cells were essentially null. Hence, it is suggested from these findings that B lymphocytes might be crucial for the continuous latency in HBV infection and may give rise to de novo acute hepatitis B if totally deleted. Moreover, the CHOP regimen might have some additive effects with the repeated on-off use of corticosteroids to the onset of the disease. In addition, significance of plasma cell infiltration in this setting is discussed.

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Classification of hepatitis B virus genotypes by the PCR-Invader method with genotype-specific probes

Cited by 43 publications

References 20 publications

Direct Comparison of 3 PCR Methods in Detecting EGFR Mutations in Patients with Advanced Non–Small-Cell Lung Cancer

Direct Comparison of 3 PCR Methods in Detecting EGFR Mutations in Patients with Advanced Non–Small-Cell Lung Cancer

Clinical Features and Hepatitis B Virus (HBV) Genotypes in Pregnant Women Chronically Infected with HBV

A case of lamivudine-sensitive de novo acute hepatitis B induced by rituximab with the CHOP regimen for diffuse large B cell lymphoma

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