Classification of haematopoietic cells and haemocytes in Chinese prawn Fenneropenaeus chinensis

Zhang, Zhi Feng; Shao, Mingyu; Kang, Kyoung Ho

doi:10.1016/j.fsi.2005.11.003

Cited by 49 publications

(22 citation statements)

References 20 publications

(27 reference statements)

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“…However, in different species, these cells display variation in number and size of granules. For instance, numerous small granules were reported in the hyaline cells of H. americanus while only a few large granules were spotted in the hyaline cells of Penaeus paulensis (Gargioni and Barracco, 1998) and F. chinensis (Zhang et al, 2006). In L. grandis, the hyaline cells are rich in granules that they are easily confused with granulocytes while in P. interruptus, the granules in hyaline and large granule haemocytes are approximately of the same size.…”

Section: Discussionmentioning

confidence: 99%

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Padmanabhan¹,

Raghavan²,

Kenchappa³

2017

Turk. J. Fish. Aquat. Sci.

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The light and ultrastructural observations on haemocyte profile of the freshwater crab Travancoriana schirnerae demonstrated three cell types: agranulocyte, granulocyte I and granulocyte II. Agranulocytes are the smallest of the haemocyte types, comprised 38% of the haemocytes, devoid of granules and showed high nucleoprotoplasmic ratio. Their cytoplasm contained numerous free ribosomes, vesicles of smooth endoplasmic reticulum, few mitochondria with parallel cristae and vacuoles. Granulocytes I (37%) are the largest of the haemocyte types, characterized by the presence of numerous granules (26 to 61) of varying size, shape and densities and organelles like mitochondria, free ribosomes and peripheral vacuoles and displayed low nucleoprotoplasmic ratio. Granulocytes II (25%) contained large electron dense granules (10 to 23) and organelles like free ribosomes and vacuoles and showed intermediate nucleoprotoplasmic ratio. Unlike granulocytes, agranulocytes exhibited prominent pseudopodial projections suggesting a phagocytory function. This study revealed the unusual occurrence of a binucleate agranulocyte and a rare phenomenon called clasmatosis of granulocyte II, wherein part of the cell fragments off along with cytoplasmic contents devoid of nucleus.

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Section: Discussionmentioning

confidence: 99%

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Padmanabhan¹,

Raghavan²,

Kenchappa³

2017

Turk. J. Fish. Aquat. Sci.

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“…The circulating and tissue-associated hemocytes correspond mostly to granular hemocytes, including large (lengths ranging between 0.8 μm to 1.1 μm) and small (lengths ranging between 0.3 μm to 0.4 μm) granular variants, which have been characterized by transmission electron microscopy. However, additional histological studies are necessary for a complete characterization, in view of the possibility that these cells correspond to intermediates during cellular differentiation ( Van de Braak et al 2002;Zhang et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Concholepas hemocyanin biosynthesis takes place in the hepatopancreas, with hemocytes being involved in its metabolism

et al. 2010

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Hemocyanins are copper-containing glycoproteins in some molluscs and arthropods, and their best-known function is O(2) transport. We studied the site of their biosynthesis in the gastropod Concholepas concholepas by using immunological and molecular genetic approaches. We performed immunohistochemical staining of various organs, including the mantle, branchia, and hepatopancreas, and detected C. concholepas hemocyanin (CCH) molecules in circulating and tissue-associated hemocytes by electron microscopy. To characterize the hemocytes, we purified them from hemolymph. We identified three types of granular cells. The most abundant type was a phagocyte-like cell with small cytoplasmic granules. The second type contained large electron-dense granules. The third type had vacuoles containing hemocyanin molecules suggesting that synthesis or catabolism occurred inside these cells. Our failure to detect cch-mRNA in hemocytes by reverse transcription with the polymerase chain reaction (RT-PCR) led us to propose that hemocytes instead played a role in CCH metabolism. This hypothesis was supported by colloidal gold staining showing hemocyanin molecules in electron-dense granules inside hemocytes. RT-PCR analysis, complemented by in situ hybridization analyses with single-stranded antisense RNAs as specific probes, demonstrated the presence of cch-mRNA in the hepatopancreas; this was consistent with the specific hybridization signal and confirmed the hepatopancreas as the site of CCH synthesis. Finally, we investigated the possibility that CCH catabolism in hemocytes was involved in the host immune response and in the generation of secondary metabolites such as antimicrobial peptides and phenoloxidase.

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“…It is commonly believed that crustacean hemocytes originate from specialized hematopoietic tissue (HPT) (Zhang et al, 2006). According to the characteristic, the cells of HPT can be classified into five types (Noonin et al, 2012;Lin and Söderhäll, 2012).…”

Section: Differentiation Of Crustacean Hemocytesmentioning

confidence: 99%

“…Crustacean hemocytes are generally divided into three main types based on the number of cytoplasmic granules in the haemocytes and immunochemical criteria, i.e. the hyaline cells (HC), semigranular cells (SGC), and granular cells (GC) (Johansson and Söderhäll 1988;Van De Braak et al, 2002;Zhang et al, 2006;Lin and Söderhäll, 2012).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural and immunocytochemical analysis of circulating hemocytes from Cherax quadricarinatus (von Martens, 1868)

Luo

Zeng

Zhao

et al. 2016

IJAR

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Transmission electron microscopy (TEM) and immunocytochemistry were used to assay the circulating hemocytes characteristics in Cherax quadricarinatus (von Martens, 1868). Three haemocyte types could be identified in circulating hemocytes by TEM, i.e. hyaline cells (HC), semigranular cells (SGC), or granular cells (GC). The circulating hemocytes from C. quadricarinatus could be separated into layers by density gradient centrifugation. The CD34+ cells which are believed to be stem cell-like cells has been discovered in the first layer via immunocytochemical assay, which has not been reported in previous studies. These CD34+ cells may be immature cells derived from a single undifferentiated precursor cell line in specialized hematopoietic tissue (HPT).

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Classification of haematopoietic cells and haemocytes in Chinese prawn Fenneropenaeus chinensis

Cited by 49 publications

References 20 publications

Untitled

Untitled

Concholepas hemocyanin biosynthesis takes place in the hepatopancreas, with hemocytes being involved in its metabolism

Ultrastructural and immunocytochemical analysis of circulating hemocytes from Cherax quadricarinatus (von Martens, 1868)

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