Classification of Grass Fructans by 13C NMR Spectroscopy.

Hammer, Hans; Morgenlie, Svein; Hammershøi, Anders; Hammershøi, B. S.; Larsen, S.; Wilgocki, M.; Wold, Svante

doi:10.3891/acta.chem.scand.44-0158

Cited by 22 publications

(15 citation statements)

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“…1c). 13 C chemical shift assignments of both native levan and fructose-oligosaccharides were highly homologous to those obtained for linear L-(2,6)-linked levans of rough meadowgrass [15] and were clearly distinguishable from spectra of branched grass fructans (data not shown). In vitro digestion experiments demonstrated that the L-(2,6)-FOS are highly resistant towards enzymes of the upper gastrointestinal tract.…”

Section: Preparation and Analyses Of L-(26)-fosmentioning

confidence: 56%

“…The production of su¤cient substrate for all experiments was achieved by mild acid hydrolysis and puri-¢cation by ion-exchange chromatography. The product contained oligosaccharides with a DP in the range of 22 0 and the L-(2,6) linkage type was con¢rmed by comparison of 13 C-NMR data with those published for linear grass levan [15]. As already shown for inulin-type oligomers [18] and wheat fructans [17], the long-chain L-(2,6)-FOS were more resistant against acid or enzymatic hydrolysis than the short-chain L-(2,6)-FOS (Fig.…”

Section: Discussionmentioning

confidence: 78%

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Metabolization of β-(2,6)-linked fructose-oligosaccharides by different bifidobacteria

Marx¹

2000

FEMS Microbiology Letters

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Low‐molecular‐mass β‐(2,6)‐linked fructose‐oligosaccharides (β‐(2,6)‐FOS) were examined as a new carbohydrate source for growth of bifidobacteria. β‐(2,6)‐FOS were prepared from microbial high‐molecular‐mass levan by acid hydrolysis and refined by cation‐exchange chromatography. 13C‐NMR spectroscopy confirmed the presence of predominantly β‐(2,6)‐fructosyl linkages in the oligosaccharides. More than 80%β‐(2,6)‐FOS was recovered after in vitro incubation with amylolytic and proteolytic enzymes, implying resistance to degradation in the upper intestinal tract. Bifidobacterium adolescentis, B. longum, B. breve, and B. pseudocatenulatum were studied in vitro for their ability to metabolize β‐(2,6)‐FOS. Growth, decrease in pH, formation of short‐ chain fatty acids (lactate, acetate, formate) and degradation of β‐(2,6)‐FOS were markedly different among species. B. adolescentis showed the best growth, produced the highest amounts of organic acids and metabolized both short‐ and long‐chain β‐(2,6)‐FOS.

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Section: Preparation and Analyses Of L-(26)-fosmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 78%

Metabolization of β-(2,6)-linked fructose-oligosaccharides by different bifidobacteria

Marx¹

2000

FEMS Microbiology Letters

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“…There are many reports dealing with the characterization of 13 C-signals for fructan Bancal et al, 1991;Hammer and Morgenile, 1990;Heyer et al, 1998). According to former assignments, C-1 signal of glucose ranges between 93 and 94 ppm, around which we found two Fig.…”

Section: Residuesmentioning

confidence: 61%

Structure of polysaccharide from Polygonatum cyrtonema Hua and the antiherpetic activity of its hydrolyzed fragments

Liu

Meng

et al. 2004

Antiviral Research

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“…Poa trivialis has linear long-chain Iriictans. while the fructans in the roots from Agropyron repens have an extensively branched structure (Hammer and Morgenlie 1990). The distribution of olieosaccharides and fructans in grasses.…”

Section: Resultsmentioning

confidence: 99%

Effects of photoperiod and temperature on sugars and fructans in leaf blades, leaf sheaths and stems, and roots in relation to growth of Poa pratensis

Solhaug¹

1991

Physiologia Plantarum

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Solhaug, K. A. 1991. Effects of photoperiod and temperature on sugars and fructans in leaf blades, leaf sheaths and stems, and roots in relation to growth of Poa pratensis. Vegetative piants of Poa pratensis L. cv. Holt were cultivated in short days (SD; 8 h summer daylight) and in iong days (LD; 8 h summer daylight + iow intensity extension of 5 (imol m • s ') at 12, 18 and 24°C in one experiment and at 9,12,15 and 18°C in another. Relative growth rate (RGR) as the mean of both experiments and all temperatures was 32% higher in LD than in SD between start of daylength treatment and first harvest, and 18% higher in LD than in SD between first and second harvest. Early in the daylength treatment period, more assimilates were allocated to storage in SD than in LD, so that at first harvest leaf sheaths and stems had 175% higher concentration of fructans in SD. Later this aliocatlon pattern changed, and for the larger plants at the second harvest the differences in fructan concentrations were much smaller between the two daylengths. Both sugar and fructan concentrations were highest at low temperatures. The distribution of sugars and fructans varied from mostly sugars in the leaves to mostly fructans in leaf sheaths and stems and roots. The fructans were mainly high degree polymerization fruclans. At least two series of fructans were present, and the dominant one was probably leased upon kestose. It is concluded that allocation of assimilates to growth in leaf area instead of to storage may be important for Ihe observed LD stimulation of dry matter production.

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Classification of Grass Fructans by 13C NMR Spectroscopy.

Cited by 22 publications

References 0 publications

Metabolization of β-(2,6)-linked fructose-oligosaccharides by different bifidobacteria

Metabolization of β-(2,6)-linked fructose-oligosaccharides by different bifidobacteria

Structure of polysaccharide from Polygonatum cyrtonema Hua and the antiherpetic activity of its hydrolyzed fragments

Effects of photoperiod and temperature on sugars and fructans in leaf blades, leaf sheaths and stems, and roots in relation to growth of Poa pratensis

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