Classification of food spoilage bacterial species and their sodium chloride, sodium acetate and glycine tolerance using chemometrics analysis and Raman spectroscopy

Yamamoto, Takashi; Taylor, J. Nicholas; Koseki, Shige; Koyama, Kento

doi:10.1016/j.mimet.2021.106326

Cited by 13 publications

(8 citation statements)

References 27 publications

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“…Sodium acetate (C 2 H 3 NaO 2 ) is a cheap, broadly available and non-toxic salt of acetic acid, which can be used in biology as the carbon source for culturing bacteria and for increasing yields of DNA isolation by ethanol precipitation. Sodium acetate can be added to food as a seasoning in the form of sodium diacetate, a one-to-one complex of sodium acetate and acetic acid [29]. It is also used to confer a salt and vinegar flavor to potato chips or even as a vinegar substitute since it doesn't increase the moisture content of the product [30].…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of Sodium Acetate Treatment on the Mechanical Properties and Morphology of Natural Fiber-Reinforced Composites

et al. 2021

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This paper aims to investigate the ability of an eco-friendly and cheap treatment based on sodium acetate solutions to improve the mechanical properties of flax fiber-reinforced composites. Flax fibers were treated for 5 days (i.e., 120 h) at 25 °C with mildly alkaline solutions at 5%, 10% and 20% weight content of the sodium salt. Quasi-static tensile and flexural tests, Charpy impact tests and dynamical mechanical thermal (DMTA) tests were carried out to evaluate the mechanical properties of the resulting composites. Fourier transform infrared analysis (FTIR) was used to evaluate the chemical modification on the fibers surface due to the proposed treatment, whereas scanning electron microscope (SEM) and helium pycnometry were used to get useful information about the morphology of composites. It was found that the treatment with 5% solution of sodium acetate leads to the best mechanical performance and morphology of flax fiber-reinforced composites. SEM analysis confirmed these findings highlighting that composites reinforced with flax fibers treated in 5% sodium acetate solution show an improved morphology compared to the untreated ones. On the contrary, detrimental effects on the morphology as well as on the mechanical performance of composites were achieved by increasing the salt concentration of the treating solution.

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Section: Introductionmentioning

confidence: 99%

Effectiveness of Sodium Acetate Treatment on the Mechanical Properties and Morphology of Natural Fiber-Reinforced Composites

et al. 2021

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“…The database can then be used for the identification of unknown samples [45]. Compared to genotypic methods, such as NGS, Raman spectroscopy is a relatively rapid and inexpensive method for the classification of bacterial species [47].…”

Section: Raman Spectroscopymentioning

confidence: 99%

Oxygen Sensor-Based Respirometry and the Landscape of Microbial Testing Methods as Applicable to Food and Beverage Matrices

Papkovsky

Kerry

2023

Sensors

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The current status of microbiological testing methods for the determination of viable bacteria in complex sample matrices, such as food samples, is the focus of this review. Established methods for the enumeration of microorganisms, particularly, the ‘gold standard’ agar plating method for the determination of total aerobic viable counts (TVC), bioluminescent detection of total ATP, selective molecular methods (immunoassays, DNA/RNA amplification, sequencing) and instrumental methods (flow cytometry, Raman spectroscopy, mass spectrometry, calorimetry), are analyzed and compared with emerging oxygen sensor-based respirometry techniques. The basic principles of optical O2 sensing and respirometry and the primary materials, detection modes and assay formats employed are described. The existing platforms for bacterial cell respirometry are then described, and examples of particular assays are provided, including the use of rapid TVC tests of food samples and swabs, the toxicological screening and profiling of cells and antimicrobial sterility testing. Overall, O2 sensor-based respirometry and TVC assays have high application potential in the food industry and related areas. They detect viable bacteria via their growth and respiration; the assay is fast (time to result is 2–8 h and dependent on TVC load), operates with complex samples (crude homogenates of food samples) in a simple mix-and-measure format, has low set-up and instrumentation costs and is inexpensive and portable.

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“…In general, spikes rarely appear at the same shift in the Raman spectrum of the same sample [96]. In this regard, we can judge whether there is a spike by visually inspecting and comparing the difference in abnormal intensity between different spectral curves [97]. In addition, setting the signal intensity threshold and deriving the spectral data can also achieve the purpose of removing spikes [98].…”

Section: Raman Spectroscopy Preprocessingmentioning

confidence: 99%

“…It is used to deal with the situation of large signal strength caused by uneven sample distribution, laser power difference, experimental environment interference and other factors [104]. Vector normalization is one of the most commonly used normalization methods in Raman spectral analysis [97,106], It is used to control the difference in Raman signal intensity levels by mapping the data to a range of 0 to 1 for processing [107]. It is worth noting that the order of preprocessing is not fixed and each step does not necessarily need to be performed.…”

Section: Raman Spectroscopy Preprocessingmentioning

confidence: 99%

Recent Progress in the Diagnosis of Staphylococcus in Clinical Settings

Zhang¹,

Gu²,

Usman³

et al. 2023

Infectious Diseases

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Staphylococci are mainly found on the skin or in the nose. These bacteria are typically friendly, causing no harm to healthy individuals or resulting in only minor issues that can go away on their own. However, under certain circumstances, staphylococcal bacteria could invade the bloodstream, affect the entire body, and lead to life-threatening problems like septic shock. In addition, antibiotic-resistant Staphylococcus is another issue because of its difficulty in the treatment of infections, such as the notorious methicillin-resistant Staphylococcus aureus (MRSA) which is resistant to most of the currently known antibiotics. Therefore, rapid and accurate diagnosis of Staphylococcus and characterization of the antibiotic resistance profiles are essential in clinical settings for efficient prevention, control, and treatment of the bacteria. This chapter highlights recent advances in the diagnosis of Staphylococci in clinical settings with a focus on the advanced technique of surface-enhanced Raman spectroscopy (SERS), which will provide a framework for the real-world applications of novel diagnostic techniques in medical laboratories via bench-top instruments and at the bedside through point-of-care devices.

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Classification of food spoilage bacterial species and their sodium chloride, sodium acetate and glycine tolerance using chemometrics analysis and Raman spectroscopy

Cited by 13 publications

References 27 publications

Effectiveness of Sodium Acetate Treatment on the Mechanical Properties and Morphology of Natural Fiber-Reinforced Composites

Effectiveness of Sodium Acetate Treatment on the Mechanical Properties and Morphology of Natural Fiber-Reinforced Composites

Oxygen Sensor-Based Respirometry and the Landscape of Microbial Testing Methods as Applicable to Food and Beverage Matrices

Recent Progress in the Diagnosis of Staphylococcus in Clinical Settings

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