Classification of Fluorescent Soft Rot Pseudomonas Bacteria, Including P. marginalis Strains, Using Whole Cell Fatty Acid Analysis

Janse, J.; Derks, J. H. J.; Spit, B. E.; Tuin, W.R. Van Der

doi:10.1016/s0723-2020(11)80114-1

Cited by 42 publications

(28 citation statements)

References 35 publications

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“…marginalis is regarded as one of many oxidase positive, soft rot fluorescent pseudomonads (Lelliott et al 1966;Janse et al 1992). Recently, based on 16S rRNA analysis, P. marginalis has been placed in the P. fluorescens group (Anzai et al 2000).…”

Section: Discussionmentioning

confidence: 99%

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Pseudomonas corrugata and Pseudomonas marginalis associated with the collapse of tomato plants in rockwool slab hydroponic culture

Kůdela¹,

Krejzar²,

Pánková³

2010

Plant Prot. Sci.

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Kůdela V., Krejzar V., Pánková I. (2010): Pseudomonas corrugata and Pseudomonas marginalis associated with the collapse of tomato plants in rockwool slab hydroponic culture. Plant Protect. Sci., 46: 1-11.Plant pathogenic species Pseudomonas corrugata and P. marginalis were detected and determined in collapsed tomato plants in rockwool slab hydroponic culture in southern Moravia, Czech Republic. Surprisingly, P. marginalis was also determined before planting in apparently healthy grafted tomato transplants grown in hydroponic culture. Moreover, non-pathogenic P. fluorescens, P. putida, P. synxantha, and Stenotrophomonas malthophilia were identified. The Biolog Identification GN2 MicroPlate TM System (Biolog, Inc., Hayward, USA)was used for identification of bacterial isolates. Cultures of P. corrugata and P. marginalis were used in a greenhouse pathogenicity experiment. Seven weeks old tomato plants of cv. Moneymaker grown in sterilised perlite were inoculated into the stem with a hypodermic needle at one point above the cotyledon node. In inoculated tomato plants, disease symptoms were observed that included external and internal dark brown lesions around the inoculation site, watering and collapse of pith and sometimes also vascular browning and wilting of leaves. In comparison with P. marginalis, P. corrugata appeared to be a much stronger pathogen. Both tested Pseudomonas species were recovered from inoculated tomato plants. P. corrugata was found to move both upwards to the apex of the stem and downwards from the site of the inoculated stem into roots. When inoculated into potato tuber slices, some tomato strains of P. marginalis, P. fluorescens, P. synxantha, and Pseudomonas sp. produced soft rot. However, other strains of the same species were not able to macerate the potato tissue. It is concluded that P. corrugata and P. marginalis can be associated with the collapse of tomato crop in soilless culture grown in a greenhouse. This is the first report on P. corrugata in tomato plants in the Czech Republic. The role of plant pathogenic bacteria, fungal root rot and vascular pathogens and Pepino mosaic virus in the collapse of tomato plants is discussed.

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Section: Discussionmentioning

confidence: 99%

“…As regards P. marginalis (sensu stricto), this fluorescent pseudomonad serves as a typical species indicating the positive pectolytic activity. Potato-macerating isolates among the fluorescent pseudomonads represented a continuum of phenotypes from P. fluorescent to Pseudomonas putida (Sands & Hankin 1975;Janse et al 1992).…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas corrugata and Pseudomonas marginalis associated with the collapse of tomato plants in rockwool slab hydroponic culture

Kůdela¹,

Krejzar²,

Pánková³

2010

Plant Prot. Sci.

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“…tolaasii has routinely been distinguished from other pseudomonads by its ability to cause dark brown discolorations on mushrooms and by a positive WLA with P. reactans (18,36,46,61,65). However, an earlier study (12) identified a mushroom farm isolate, Pseudomonas NZI7, as being WLAϩ (with P. reactans) and causing discoloration of A. bisporus tissue comparable to that caused by P. tolaasii.…”

Section: Discussionmentioning

confidence: 99%

Characterization by 16S rRNA Sequence Analysis of Pseudomonads Causing Blotch Disease of Cultivated Agaricus bisporus

Godfrey

Harrow

Marshall

et al. 2001

Appl Environ Microbiol

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Bacterial blotch of Agaricus bisporus has typically been identified as being caused by either Pseudomonas tolaasii (brown blotch) or Pseudomonas gingeri (ginger blotch). To address the relatedness of pseudomonads able to induce blotch, a pilot study was initiated in which pseudomonads were selectively isolated from mushroom farms throughout New Zealand. Thirty-three pseudomonad isolates were identified as being capable of causing different degrees of discoloration (separable into nine categories) of A. bisporus tissue in a bioassay. These isolates were also identified as unique using repetitive extragenic palindromic PCR and biochemical analysis. Relationships between these 33 blotch-causing organisms (BCO) and a further 22 selected pseudomonad species were inferred by phylogenetic analyses of near-full-length 16S rRNA gene nucleotide sequences. The 33 BCO isolates were observed to be distributed throughout the Pseudomonas fluorescens intrageneric cluster. These results show that in addition to known BCO (P. tolaasii, P. gingeri, and Pseudomonas reactans), a number of diverse pseudomonad species also have the ability to cause blotch diseases with various discolorations. Furthermore, observation of ginger blotch discoloration of A. bisporus being independently caused by many different pseudomonad species impacts on the homogeneity and classification of the previously described P. gingeri.The genus Pseudomonas (sensu stricto) comprises a taxon of metabolically versatile organisms that are ubiquitous in soil and water and play an important role as plant, animal, and human pathogens (37). Microbial diversity in mushroom farms has previously been reported, with pseudomonads accounting for 10% of bacteria in compost and sometimes more than 50% of bacteria in casing soils (48).Discoloration of Agaricus bisporus caused by pathogenic pseudomonads, the so-called blotch diseases, are well documented. Pseudomonas tolaasii contamination results in sunken, dark brown lesions (35, 55); Pseudomonas reactans causes mild dark purple to light brown discoloration and a slight surface depression that becomes deeper and darker with age (59); while the pale yellowish red discoloration that develops into a reddish ginger-colored discoloration (ginger blotch disease) is characteristic of Pseudomonas gingeri (60).Of the blotch-causing pseudomonads, the best characterized is P. tolaasii. P. tolaasii enters the mushroom farm in peat and limestone used in the casing process (63), and, once present, P. tolaasii is able to attach to mycelial surfaces of developing A. bisporus (40, 42). Temperature and relative humidity have been suggested as important environmental conditions that influence the pathogenicity of P. tolaasii within the mushroom farm (50). A minimal application of 2.7 ϫ 10 6 to 10 8 CFU ⅐ ml Ϫ1 of P. tolaasii was reported as the threshold for inducing disease (34), although other thresholds have been proposed (31,44,62). Pathogenic P. tolaasii isolates synthesize a lowmolecular-weight extracellular toxin, tolaasin, that is the p...

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“…For example a new isolate that falls somewhere within the "fluorescens supercluster" would be expected to share a limited set of features exhibited by all the members of that group, even though it may not be possible to tell within which species or biovar of that group the new isolate belongs. As an illustration from a risk assessment perspective, a new isolate that is identified as a biovar of P. fluorescens might merit further inquiry and/or testing, since the subgroup, P. fluorescens biovar II, has embedded within it some members previously known as P. marginalis, which is a known plant pathogen (Janse et al, 1992). Since not all P. marginalis-like isolates fit within the boundaries of P. fluorescens biovar II, one cannot automatically assume that only biovar II isolates are likely to be pathogenic, nor can one assume that biovar II isolates must be pathogenic, but the direction of inquiry can be focused by knowing that the isolate falls within the "supercluster" and can be enhanced if own can narrow down that information to one of the categorized biovars of P. fluorescens.…”

Section: Use Of Taxonomy As a Basis For Further Inquiriesmentioning

confidence: 99%

Safety Assessment of Transgenic Organisms, Volume 4

2010

Harmonisation of Regulatory Oversight in Biotechnology

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io s a fe t y nv ir on me nt ag ri cu lt ur e bi os af et y g r ic u lt u r e e n vi r o n m e n t gr ic ul tu re bi os af et y en vi ro nm en t n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en v io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm e g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f gr ic ul tu re bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t a n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f gr ic ul tu re bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t a n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y e g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et...

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Classification of Fluorescent Soft Rot Pseudomonas Bacteria, Including P. marginalis Strains, Using Whole Cell Fatty Acid Analysis

Cited by 42 publications

References 35 publications

Pseudomonas corrugata and Pseudomonas marginalis associated with the collapse of tomato plants in rockwool slab hydroponic culture

Pseudomonas corrugata and Pseudomonas marginalis associated with the collapse of tomato plants in rockwool slab hydroponic culture

Characterization by 16S rRNA Sequence Analysis of Pseudomonads Causing Blotch Disease of Cultivated Agaricus bisporus

Safety Assessment of Transgenic Organisms, Volume 4

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